Establishment of new treatment for cleft lip and palate patients-basic research for the application of in vitro hybrid artificial bone-

唇腭裂患者新疗法的建立——体外混合型人工骨应用基础研究——

• 批准号: 12557185
• 负责人: MORIYAMA Keiji
• 金额: $ 4.93万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12557185/
• 关键词: cleft lip and palate; autograft of iliac crest cancellous bone; collagen sponge; soft X-ray; artificial bone marrow; 自家腸骨海面骨移植術; 架橋ハイドロゲル; FGF-2; BMP; soft X-ray

Currently, autograft of iliac crest cancellous bone has become the most commonly utilized method for restoration of the alveolar cleft. In recent years, we have identified the cleft / nasal cavity ratio analyzed by presurgical frontal cephalograms and the residual cortical bone ratio, which was analyzed by CT taken at 3 months after SBG, as parameters affecting the clinical outcome of the autograft of iliac crest cancellous bone. However, several clinical outcomes of autograft of iliac crest cancellous bone for cleft lip and palate patients, were not corresponded to our previously studies. Therefore, it is of imperative importance to investigate the new donor materials, such as the artificial bone which might be a new method for restoration of the alveolar cleft. The stromal cells of rabbit bone marrow were three-dimensionally cultured in collagen sponge treated with four cytokines (FGF2, BMP-2, 4, 7) in vitro. The collagen sponge treated with FGF-2 showed increased matrix mineralization as compared with BMP-2, 4, 7. Furthermore, in order to confirm the effect of FGF-2 in vivo, stromal cells of rabbit bone marrow were injected into collagen sponge treated with or without FGF-2 and these complexes were grafted in round defects (diameter; 7.0 mm) artificially created in rabbit calvarial bone. Longitudinal evaluation of grafted site showed significant ossification of collagen sponge treated with FGF2. Therefore, these results suggested that new treatment method utilized FGF2 might be useful in the treatment of cleft lip and palate patients.

目前，自体髂嵴松质骨移植已成为牙槽裂修复最常用的方法。近年来，我们将术前额头照片分析的裂隙/鼻腔比值和SBG术后3个月CT分析的残余骨皮质比值确定为影响自体髂嵴松质骨移植临床效果的参数。骨。然而，唇腭裂患者自体髂嵴松质骨移植的一些临床结果与我们之前的研究并不相符。因此，研究新的供体材料刻不容缓，例如人工骨，它可能成为修复牙槽裂的新方法。将兔骨髓基质细胞在体外用四种细胞因子（FGF2、BMP-2、4、7）处理的胶原海绵进行三维培养。与BMP-2、4、7相比，经FGF-2处理的胶原海绵显示出基质矿化增加。此外，为了证实FGF-2在体内的作用，将兔骨髓基质细胞注射到经FGF-2处理的胶原海绵中。有或没有FGF-2，这些复合物被移植到兔颅骨中人工产生的圆形缺损（直径；7.0毫米）中。移植部位的纵向评估显示经 FGF2 处理的胶原海绵显着骨化。因此，这些结果表明利用 FGF2 的新治疗方法可能有助于治疗唇腭裂患者。

项目成果

Tateishi C.: "Dentocraniofacial morphology of 12 japanese subjects with unilateral cleft lip and palate with a severe class III malocclusion"Cleft Palate Craniofac. J.. 38・6. 597-605 (2001)

Tateishi C.：“12 例日本单侧唇腭裂患者的牙颌面形态，伴有严重的 III 类咬合不正”Cleft Palate Craniofac. 38・6 (2001)。

Yasue,A.: "The role of myostatin during myogenesis"SEIMEI NO KAGAKU. 52 (4). 256-260 (2001)

Yasue,A.：“肌生成抑制素在肌生成过程中的作用”《SEIMEI NO KAGAKU》。

泰江章博: "骨格筋形成におけるマイオスタチンの役割"生命の科学. 52(4). 256-260 (2001)

Akihiro Yasue：“肌肉生长抑制素在骨骼肌形成中的作用”《生命科学》52(4)。

Takeishi,C.: "Dentocraniofacial morphology of 12 Japanese subjects with unilateral cleft lip and palate with a severe class III malocclusion"Cleft Palate Craniofac. J.. 38 (6). 597-605 (2001)

Takeishi,C.：“12 名患有单侧唇腭裂且严重 III 类咬合不正的日本受试者的牙颌面形态”Cleft Palate Craniofac。

Okamoto M.: "Mechanism for bone invasion of oral cancer cells mediated by nterleukin-6 in vitro and in vivo"Cancer. 89・1. 1966-1975 (2000)

冈本 M.：“体内和体外介导的口腔癌细胞骨侵袭机制”癌症 89·1（2000）。