REGULATION OF OSTEOCLASTIC BONE RESORPTION BY RHO

RHO 对破骨性骨吸收的调节

基本信息

批准号：
11694283
负责人：
MORIYAMA Keiji
金额：
$ 2.05万
依托单位：
THE UNIVERSITY OF TOKUSHIMA
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

项目摘要

At the time when a mechanical force is applied onto the tooth, mechanical stress is loaded on the periodontal tissues. A number of osteocytes are located in the bone matrix, communicating each other, as well as with the osteoblasts on the bone surface, via cytoplasmatic processes and gap junctions. Such a cellular network is speculated to have certain important functions for the adaptation to a mechanical loaded environment by the detection of mechanical stress.In the present study, in order to investigate the role of osteocytes in bone resorption, we examined the effects of purified chick osteocyte-derived protein (ODP) and Rho-GTP-dissociation inhibitor (Rho-GDI) on bone resorption. Recently, we established a culture system which provided with human mature osteoclasts from peripheral mononuclear blood cells (PMBC) by using M-CSF and human soluble RANKL (sRANKL) in the absence of osteoblasts and stromal cells. FITC-labelled alpha-actinin pre-microinjected into human osteoclasts was in … More corporated into podosomes which lined the periphery of the cells. Although the podosomes were still observed after the injection of buffer, microinjection of ODP and Rho-GDI caused disruption of podosomes within 30 min of injection. Furthermore, both proteins markedly inhibited pit formation, whereas the conditioned medium of osteocytes had no effect.This study has also focused on a novel cysteine proteinase, cathepsin K, which is through to be specifically expressed in osteoclasts and plays an important part in osteoclastic bone resorption. We have investigated the mechanism of alveolar bone remodelling during orthodontic tooth movement based on an examination of changes in cathepsin K mRNA expression in parallel with the histological changes in alveolar bone.Therefore, further insights into osteoclasts differentiation and function mechanisms may provide stronger bases that will help to understand with more detail the fundaments of orthodontic tooth movement and the reaction of mechanically stressed periodontal tissues. Less

当机械力施加到牙齿上时，牙周组织上会承受机械应力，许多骨细胞位于骨基质中，通过细胞质相互沟通以及与骨表面的成骨细胞沟通。推测这种细胞网络通过检测机械应力具有适应机械负载环境的某些重要功能。在本研究中，为了研究骨细胞在骨吸收中的作用，我们检查了骨细胞。的影响纯化的鸡骨细胞衍生蛋白（ODP）和Rho-GTP解离抑制剂（Rho-GDI）对骨吸收的影响最近，我们建立了一个培养系统，该系统利用M-从外周单核血细胞（PMBC）提供人成熟破骨细胞。在没有成骨细胞和基质细胞的情况下将 CSF 和人可溶性 RANKL (sRANKL) 预先显微注射到人破骨细胞中。尽管在注射缓冲液后仍可观察到足小体，但显微注射 ODP 和 Rho-GDI 在注射后 30 分钟内引起了足小体的破坏。此外，两种蛋白均受到抑制。凹坑的形成，而骨细胞的条件培养基没有影响。这项研究还关注了一种新型半胱氨酸蛋白酶，组织蛋白酶K，它通过在破骨细胞在破骨细胞骨吸收中发挥重要作用，我们通过检查组织蛋白酶 K mRNA 表达与牙槽骨组织学变化的平行变化，研究了正畸牙齿移动过程中牙槽骨重塑的机制。破骨细胞的分化和功能机制可能提供更强有力的基础，有助于更详细地了解正畸牙齿移动的基础和机械应力牙周组织的反应。