The function of periostin during periodontal tissue remodeling caused by mechanical stress.

骨膜素在机械应力引起的牙周组织重塑过程中的功能。

基本信息

批准号：
13470452
负责人：
MORIYAMA Keiji
金额：
$ 10.37万
依托单位：
The University of Tokushima
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

项目摘要

The periodontal ligament (PDL) plays an important role in the remodeling of the periodontal tissues during orthodontic tooth movement. Many factors are involved in the mechanisms of response to an orthodontic force. One of these factors might be periostin, which has a specific expression in the PDL and the periosteum. Therefore, the aim of this study was to elucidate the role of periostin in the PDL during tooth movement and its effect on osteoclasts. In this study, periostin mRNA expression was investigated using experimental tooth movement model in rats in vivo and the effect of uni-axial mechanical stress was examined on human periodontal ligament fibroblastic cells (hPDLF) in vitro. Also the effect of periostin on osteoclasts was analyzed. In situhybridization assay revealed that periostin mRNA was expressed in the PDL in controls stained with antisense probes. This expression appeared to be stronger in the compression sites compared with the tension sites after 3 hours of starting tooth movement. At 24 hours periostin mRNA showed the highest levels and returned to levels similar to controls after 168 hours. In vitro experiments showed that periostin mRNA expression detected by RT-PCR increased after uni-axial mechanical stress was applied for 24 hours in hPDLF cultures. We also found that periostin had an inhibitory effect on osteoclast differentiation and activation when conditioned medium from mechanically stressed hPDLF was added to human osteoclast culture system. These data was confirmed when similar results were observed in mouse unfractionated bone cell cultures treated with mouse recombinant periostin to a final concentration of 600 ng/ml. In this study, we demonstrated that periostin mRNA expression increased in the PDL during experimental tooth movement in vivo, especially in the compression sites, and was induced by uni-axial mechanical stress in vitro. Furthermore, periostin may inhibit osteoclast differentiation and activation.

牙周韧带（PDL）在正畸牙齿移动过程中牙周组织的重塑中发挥着重要作用。对正畸力的反应机制涉及许多因素。这些因素之一可能是骨膜素，它在 PDL 和骨膜中具有特异性表达。因此，本研究的目的是阐明骨膜素在牙齿移动过程中 PDL 中的作用及其对破骨细胞的影响。在本研究中，利用大鼠体内实验性牙齿移动模型研究了骨膜素 mRNA 表达，并在体外检查了单轴机械应力对人牙周膜成纤维细胞 (hPDLF) 的影响。还分析了骨膜素对破骨细胞的影响。原位杂交测定显示，在用反义探针染色的对照中，骨膜素 mRNA 在 PDL 中表达。牙齿开始移动 3 小时后，与张力部位相比，这种表达在受压部位似乎更强。 24 小时时，骨膜蛋白 mRNA 显示出最高水平，并在 168 小时后恢复到与对照相似的水平。体外实验表明，在 hPDLF 培养物中施加单轴机械应力 24 小时后，RT-PCR 检测到的骨膜素 mRNA 表达增加。我们还发现，当机械应激 hPDLF 的条件培养基添加到人破骨细胞培养系统中时，骨膜素对破骨细胞的分化和活化具有抑制作用。当在用小鼠重组骨膜素处理至终浓度 600 ng/ml 的小鼠未分级骨细胞培养物中观察到类似结果时，这些数据得到了证实。在这项研究中，我们证明了在体内实验性牙齿移动过程中，PDL 中的骨膜蛋白 mRNA 表达增加，特别是在压缩部位，并且是由体外单轴机械应力诱导的。此外，骨膜素可以抑制破骨细胞的分化和活化。