Multi-Parameter Flow cytometer

多参数流式细胞仪

• 批准号: 457789665
• 金额: --
• 依托单位: Universität zu Lübeck
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2021
• 资助国家: 德国
• 起止时间: 2020-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/457789665?language=en
• 关键词: Multi; Parameter; Flow; cytometer

In recent years, increasingly complex questions concerning the phenotypic and functional characterization of different professional and non-professional immune cells have become the focus of research at the University of Lübeck (UzL) and the University Hospital Schleswig-Holstein (UKSH) Campus Lübeck. Flow cytometry, whose potential has increased enormously in recent years due to constant new developments, especially in the field of fluorochromes and flow cytometers, is one of the most important analytical methods in immunological research. In order to successfully work on complex problems, cytometers are required which allow multiparameter experiments to be carried out. The Cell Analysis Core Facility (CAnaCore), the technology platform for flow cytometry and cell sorting at UzL, currently operates an analytical flow cytometer with 4 lasers for the detection of 15 color parameters as well as FSC/SSC. In the meantime, multiparameter flow cytometers have been developed, which are able to simultaneously detect more than 30 color parameters and thus define the complex composition and, in addition, the function of immune cells in circulation and tissues. This makes it possible, especially with limited sample quantities, to obtain a detailed picture of immune reactions in inflammatory diseases of different origins and to derive new concepts for the diagnosis and therapy of such diseases. For this reason, a multiparameter flow cytometer is to be purchased for the CAnaCore at the University of Lübeck, which will enable researchers to carry out such state-of-the-art analyses in the many different basic and translational research projects. This concerns, in particular, the current DFG-funded joint research projects within the EXC 2167 "Precision Medicine in Chronic Inflammation", the CRU 303 “Pemphigoid Diseases - Molecular Pathways and their Therapeutic Potential", the IRTG 1911 "Immunoregulation of Inflammation in Allergy and Infection", the RTG 1743 "Genes, Environment and Inflammation", the projects of the BMBF-funded networks of the German Centers for Lung Research, (DZL), Infection Research (DZIF), Cardiovascular Research (DZHK) and Diabetes research (DZD) as well as in the EU-funded Marie Sklodowska Innovative Training Network “Complement Regulation and Variations in Opportunistic Infections” (CORVOS) and the ERC Synergy Grant “Well-Aging and the Tanycytic Control of Health” (WATCH).

近年来，有关不同专业和非专业免疫细胞的表型和功能特征的日益复杂的问题已成为吕贝克大学（UzL）和石勒苏益格-荷尔斯泰因大学医院（UKSH）吕贝克校区的研究焦点。近年来，由于不断的新发展，特别是在荧光染料和流式细胞仪领域，细胞计数法的潜力已大大增加，是免疫学研究中最重要的分析方法之一。细胞分析核心设施 (CANaCore) 是 UzL 的流式细胞术和细胞分选技术平台，目前运行一台带有 4 个激光器的分析流式细胞仪来进行检测。 15 个颜色参数以及 FSC/SSC 同时，还开发了多参数流式细胞仪，能够同时检测 30 多个颜色参数，从而定义复杂的成分。此外，免疫细胞在循环和组织中的功能使得能够获得不同来源的炎症性疾病中免疫反应的详细图像，并得出诊断和治疗此类疾病的新概念，尤其是在样本量有限的情况下。因此，吕贝克大学将为 CANaCore 购买一台多参数流式细胞仪，这将使研究人员能够在许多不同的基础和转化研究项目中进行此类最先进的分析。 ，当前的DFG 资助的联合研究项目包括 EXC 2167“慢性炎症的精准医学”、CRU 303“类天疱疮疾病 - 分子途径及其治疗潜力”、IRTG 1911“过敏和感染中炎症的免疫调节”、RTG 1743“ “基因、环境与炎症”项目BMBF 资助的德国肺研究中心 (DZL)、感染研究中心 (DZIF)、心血管研究中心 (DZHK) 和糖尿病研究中心 (DZD) 网络以及欧盟资助的 Marie Sklodowska 创新培训网络“补充调节和机会性感染的变异”(CORVOS) 和 ERC 协同资助“健康老龄化和长细胞控制” （手表）。