Multi-parameter, analytic flow cytometer

多参数分析流式细胞仪

• 批准号: 10426996
• 负责人: Troy D Randall
• 金额: $ 46.35万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-04-01 至 2023-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10426996
• 关键词: 15 year old; Antibodies; Biomedical Research; Cells; Color; Computer software; Contract Services; Department of Defense; Development; Flow Cytometry; Foundations; Funding; Grant; Hour; Human Resources; Industry; Laboratory Research; Lasers; Minor; Modification; Privatization; Research; Research Support; Schedule; Services; Specialist; Time; Training; United States National Institutes of Health; Walking; Work; base; design; experience; instrument; operation; programs

We request funds to purchase from Becton Dickinson (BD) a new 5-laser, 30-color FACSymphony A5 analytic flow cytometer. The new A5 instrument will replace our 15-year-old, 5-laser, 18-color BD LSR II instrument that BD will no longer support with a service contract after September 2022. The new A5 instrument will support the research of 14 major users and 32 minor users holding 51 NIH grants, as well as projects supported by the Department of Defense, private industry, and foundations. We will install and configure the new Symphony A5 instrument to closely match our existing Symphony A5, which is the most-used instrument in the Flow Cytometry and Single Cell services (FCSC) Core. By configuring the new A5 instrument to match the existing one, our Core personnel and users will be already trained to operate the new instrument and its onboard software. Moreover, users who already have multi-parameter antibody panels designed to work on the existing Symphony A5 can use these antibody panels on the new A5 without modification, thereby easing scheduling constraints. Based on the prior use of the existing LSR II and Symphony A5 instruments, we project that the new A5 instrument will be used 34 hours/week (85% of the Accessible User Time, AUT). The new A5 instrument will be administered by the FCSC Core and will be incorporated into the Core's existing financial plan. The FCSC Core is part of the UAB-wide Institutional Research Core Program (IRCP) and as such, the FSCS Core receives significant institutional support from the IRCP. Three highly experienced flow cytometry specialists, each trained to operate the Symphony A5, are available to train new users in instrument operation and to assist them with the development of multi-parameter antibody panels. Finally, the FCSC Core is located in the Shelby Biomedical Research Building, a building that houses the research laboratories of most of our major users and is within a short walking distance of our other users on campus. Thus, the requested new Symphony A5 instrument will fill a critical void in the FCSC Core and will allow us to continue to provide state-of-the-art instruments and services to our users.

我们请求资金从 Becton Dickinson (BD) 购买新型 5 激光、30 色 FACSymphony A5 分析仪 流式细胞仪。新的 A5 仪器将取代我们已有 15 年历史的 5 激光、18 色 BD LSR II 仪器， 2022 年 9 月后，BD 将不再支持服务合同。新的 A5 仪器将支持 对持有 51 项 NIH 拨款的 14 个主要用户和 32 个次要用户的研究，以及由 NIH 支持的项目 国防部、私营企业和基金会。我们将安装并配置新的 Symphony A5 与我们现有的 Symphony A5（流式细胞仪中最常用的仪器）非常匹配的仪器 和单小区服务 (FCSC) 核心。通过配置新的 A5 仪器以匹配现有仪器，我们的 Core 人员和用户将接受操作新仪器及其机载软件的培训。而且， 已经拥有设计用于现有 Symphony A5 的多参数抗体面板的用户可以 无需修改即可在新 A5 上使用这些抗体组，从而缓解调度限制。基于 由于之前使用了现有的 LSR II 和 Symphony A5 乐器，我们预计新的 A5 乐器将 每周使用 34 小时（可访问用户时间 (AUT) 的 85%）。新的 A5 工具将由 FCSC 核心并将纳入核心现有财务计划。 FCSC 核心是 UAB 范围内的机构研究核心计划 (IRCP)，因此 FSCS 核心获得了重要的 IRCP 的机构支持。三位经验丰富的流式细胞仪专家，每位都接受过操作培训 Symphony A5，可用于培训新用户仪器操作并帮助他们 多参数抗体组的开发。最后，FCSC 核心位于谢尔比生物医学中心 研究大楼，是我们大多数主要用户的研究实验室所在的建筑，位于 与校园内其他用户的步行距离不远。因此，所要求的新 Symphony A5 乐器将满足 FCSC 核心中的一个关键空白将使我们能够继续提供最先进的仪器和服务 给我们的用户。