Ion Mobility Spectrometry- quadrupole Time-of-Flight (IMS-qToF) Mass Spectrometer

离子淌度光谱仪 - 四极杆飞行时间 (IMS-qToF) 质谱仪

• 批准号: 10630627
• 负责人: Mary Kay H Pflum
• 金额: $ 68.5万
• 依托单位: WAYNE STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-05-01 至 2025-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10630627
• 关键词: 14 year old; 17 year old; Address; Advisory Committees; Affect; Age; Antibodies; Area; Arts; Biomedical Research; Communities; Computer software; Dedications; Development; Funding; Individual; Institution; Laboratories; Liquid Chromatography; Mass Spectrum Analysis; Medical; Methods; Mission; Modernization; Pharmaceutical Preparations; Productivity; Protein Conformation; Proteins; Request for Applications; Research; Research Activity; Research Personnel; Research Project Grants; Research Support; Resolution; Science; Scientist; Spectrometry; Strategic Planning; Structure; Technology; Time; United States National Institutes of Health; Universities; adduct; biomarker discovery; college; innovation; instrument; ion mobility; mass spectrometer; multiple omics; prevent; programs; small molecule

Summary Biomedical research in the modern age has been revolutionized by the high resolution and high sensitivity mass analysis provided by state-of-the-art mass spectrometry (MS) instruments. The Lumigen Instrument Center (LIC) at Wayne State University has a mission to provide researchers at the university and in the regional area with reliable and diverse user-based MS analysis to elevate their research productivity. As part of the strategic plan of the Mass Spectrometry Laboratory in the LIC, the Mass Spectrometry Advisory Committee identified two current challenges for users. First, the substantial down time of a 17-year old Liquid Chromatography-Time-of-Flight (LC-TOF) instrument has prevented vital high resolution mass analysis, which has dramatically affected user research productivity. Second, the outdated hardware and software of a 14 year-old LTQ Orbitrap XL has stalled application to variety of projects. Finally, development of new methods to address the unique needs of individual users is limited due to the absence of new and powerful technologies, including Ion Mobility Spectrometry (IMS). To address these challenges, this application requests funds for an Ion Mobility Spectrometry- quadrupole Time-of-Flight (IMS-qTOF) Mass Spectrometer at Wayne State University. The instrument will significantly enhance research accomplishment in mass spectrometry (MS) on our campus. First, the IMS-qTOF will replace outdated and failing MS instruments for routine high-resolution analysis of small molecules and biomolecules. Second, the instrument will provide the only mass spectrometer in a shared facility on campus with IMS capability, which will enable NIH-funded researchers to expand and transform their research activities. The multiple capabilities of the instrument will include Top-Down and Bottom-Up Protein identification, protein adduct and conjugation characterization, protein conformational studies, biomarker discovery, drug-antibody characterization, accurate mass determination, and multi-omics workflows. The instrument will support the research programs of at least 19 users at Wayne State University and the regional area, which include chemists, biologists, environmental scientists, and medical researchers. The IMS-qTOF will be housed and maintained in the Lumigen Instrument Center (LIC), which has strong institution support from the University, the Office of the Vice President of Research, and the College of Liberal Arts and Science. The LIC has an exemplary record of instrument stewardship, with dedicated staff, facilities, and management to support the long-term use of the instrument. Importantly, the user-focused structure of the LIC will promote development of a unique array of new methods for each user’s individual research projects. The acquisition of an IMS-qToF is aligned with the long-term strategic plan of the Mass Spectrometry Laboratory at the LIC, which seeks to provide state-of-the-art and reliable MS instruments to implement innovative user-initiated projects at Wayne State University and local area community.

概括 现代生物医学研究因高分辨率和高灵敏度而发生了革命性的变化 由最先进的质谱 (MS) 仪器提供质量分析。 韦恩州立大学中心 (LIC) 的使命是为大学和研究人员提供服务 区域提供可靠且多样化的基于用户的 MS 分析，以提高他们的研究生产力。 LIC 质谱实验室战略计划的一部分，即质谱咨询 委员会确定了用户当前面临的两个挑战，首先是 17 岁用户的大量停机时间。 液相色谱-飞行时间 (LC-TOF) 仪器阻止了重要的高分辨率质量 分析，这极大地影响了用户研究的生产力。 已有 14 年历史的 LTQ Orbitrap XL 软件已停止在各种项目中的应用 最后，开发。 由于缺乏新的和新的方法，满足个人用户独特需求的新方法是有限的。 强大的技术，包括离子淌度谱 (IMS)。 为了应对这些挑战，本申请请求资金用于离子淌度光谱法-四极杆 韦恩州立大学的飞行时间 (IMS-qTOF) 质谱仪将显着提高。 提高我们校园质谱（MS）的研究成果首先，IMS-qTOF 将。 更换过时且出现故障的 MS 仪器，用于小分子的常规高分辨率分析 其次，该仪器将提供校园共享设施中唯一的质谱仪。 具有 IMS 功能，这将使 NIH 资助的研究人员能够扩展和转变他们的研究 该仪器的多种功能将包括自上而下和自下而上的蛋白质。 鉴定、蛋白质加合物和缀合表征、蛋白质构象研究、生物标志物 发现、药物抗体表征、精确质量测定和多组学工作流程。 仪器将支持韦恩州立大学和韦恩州立大学至少 19 位用户的研究项目 地区，包括化学家、生物学家、环境科学家和医学研究人员。 IMS-qTOF 将在 Lumigen 仪器中心 (LIC) 进行安置和维护，该中心拥有强大的 来自大学、研究副校长办公室和学院的机构支持 文科和理科拥有堪称典范的仪器管理记录，拥有敬业的工作人员， 支持仪器长期使用的设施和管理，重要的是以用户为中心。 LIC 的结构将促进为每个用户个人开发一系列独特的新方法 收购 IMS-qToF 符合 Mass 的长期战略计划。 LIC 的光谱测定实验室致力于提供最先进且可靠的 MS 仪器 在韦恩州立大学和当地社区实施用户发起的创新项目。