Stratedigm S100EON high-parameter flow cytometer to support multi-institutional research programs

Stratigm S100EON 高参数流式细胞仪支持多机构研究项目

• 批准号: 10431674
• 负责人: Emanual M. Maverakis
• 金额: $ 59.98万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10431674
• 关键词: Biological Assay; CLIA certified; California; Centrifugation; Cities; Clinic; Clinical Research; Comprehensive Cancer Center; Core Facility; Country; Diagnostic; Flow Cytometry; Freezing; Funding; Goals; Immunological Models; Immunology; Malignant Neoplasms; Memorial Sloan-Kettering Cancer Center; Preparation; Price; Process; Research; Research Personnel; Resource Sharing; Resources; Running; Sampling; San Francisco; Services; Specimen; Stains; System; Time; Travel; United States National Institutes of Health; Universities; Work; base; cost; instrument; prevent; programs

Title: Stratedigm S100EON high-parameter flow cytometer to support multi-institutional research programs Abstract: The Immune Modeling, Analysis, and Diagnostic Shared Resource (IMAD) is a CLIA- certified core facility that performs highly validated flow cytometry for various large multi- institutional NIH-funded research programs. It is the designated immunology core facility for the California Cancer Consortium, the UC Davis Alpha Clinic, and the UC Davis Comprehensive Cancer Center. The California Cancer Consortium is an NCI-funded consortium which includes investigators from the City of Hope, University of Southern California, University of California Davis, and Stanford. The UC Davis Alpha clinic runs local and multi-institutional CIRM and NIH-sponsored clinical studies. Its partners include the University of California San Diego, University of California San Francisco, and the Memorial Sloan Kettering Cancer Center. The goal of the current NIH S10 application is to support the purchase of the Stratedigm S100EON, which is a high-parameter flow cytometer. The extremely low operating cost of this instrument and its ability to fully automate the flow cytometry work flow (i.e. staining, centrifugation, incubation, loading, etc.) make this instrument especially suited for the IMAD and separate it from other the other flow cytometers currently available. These unique features will dramatically lower the per sample acquisition cost and increase the number of samples that can processed with IMAD's existing resources. Both lowering the per sample cost and increasing the throughput capacity are essential, if the IMAD is to meet their current demand, which is evident by their large backlog of frozen specimens. As it stands, the IMAD is possibly the only immunology core in the University of California system without a dedicated flow cytometer. Also, even if the IMAD investigators are willing to travel, there are no high-parameter flow analyzers available in Sacramento for them to use. The absence of a flow cytometer is the major bottleneck that prevents the IMAD from providing exceptional pricing and turnaround time for its users. The IMAD is focused on providing high throughput, highly validated flow-based assays to investigators across the country. Finally, IMAD highly validated sample preparation and acquisition services do not overlap with current UC Davis core facilities.

标题：Stratigm S100EON高参数流式细胞仪支持多机构 研究计划 摘要：免疫建模、分析和诊断共享资源 (IMAD) 是 CLIA- 经过认证的核心设施，可为各种大型多用途执行高度验证的流式细胞术 NIH 资助的机构研究项目。它是指定的免疫学核心设施 加州癌症联盟、加州大学戴维斯分校阿尔法诊所和加州大学戴维斯分校综合医院 癌症中心。加州癌症联盟是一个由 NCI 资助的联盟，其中包括 来自希望之城、南加州大学、加州大学的研究人员 戴维斯和斯坦福大学。加州大学戴维斯分校阿尔法诊所运营本地和多机构 CIRM， NIH 资助的临床研究。其合作伙伴包括加州大学圣地亚哥分校、 加州大学旧金山分校和纪念斯隆凯特琳癌症中心。目标 当前 NIH S10 申请的主要目的是支持购买 Strategm S100EON， 是一款高参数流式细胞仪。该仪器及其运行成本极低 能够完全自动化流式细胞术工作流程（即染色、离心、孵育、 负载等）使该仪器特别适合 IMAD 并将其与其他仪器分开 目前可用的其他流式细胞仪。这些独特的功能将大大降低每 样品获取成本并增加可使用 IMAD 处理的样品数量 现有资源。降低每个样品的成本并提高吞吐量 如果 IMAD 要满足他们当前的需求（从他们大量积压的订单中可以看出这一点），这一点至关重要 冷冻标本。就目前而言，IMAD 可能是该大学唯一的免疫学核心 加州系统没有专用的流式细胞仪。此外，即使 IMAD 调查员 愿意出差，萨克拉门托没有适合他们的高参数流量分析仪 使用。缺乏流式细胞仪是阻碍 IMAD 的主要瓶颈 为其用户提供卓越的定价和周转时间。 IMAD 的重点是 为整个研究人员提供高通量、高度验证的基于流的检测 国家。最后，IMAD 高度验证的样品制备和采集服务不 与加州大学戴维斯分校当前的核心设施重叠。