Development of combination vaccine by using cytokine expressing transgenic plants.

利用表达细胞因子的转基因植物开发联合疫苗。

• 批准号: 15380198
• 负责人: MATSUMURA Takeshi
• 金额: $ 8.9万
• 依托单位: NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15380198/
• 关键词: cytokine; rotavirus; transgenic plants

Rotavirus is one of the most significant pathogens causing diarrhea in mammals. We have already produced the transgenic potato plants expressing rotavirus VP6 cDNA gene which encodes a coat protein. In addition, we have confirmed the antigenicity of the transgenic potato in mice.In this study we produced the transgenic strawberries which were introduced human interferon(hIFN) gene. Also we have produced the transgenic strawberry expressing both bovine IL-1 and rotavirus VP6 protein by using co-transformation technique. And here we examined the effect of oral administration to mice with combinations of vaccine and cytokine, using the transgenic strawberries and/or the transgenic VP6 potato mentioned above.BALB/c (8 week-old) mice were spontaneously fed with the transgenic plants once in a week, three times. After 1 week of the last(3rd) administration, those mice were challenged using rotavirus EW strain (murine group A) with dose of 10^5 TCID50.Virus antigen and anti-virus antibody in blood and feces of the challenged mice were examined. Secretory IgA was detected in feces of VP6 administrated mice. On the other hand, there was no significant difference in immune-markers among the hlFN strawberry, the IL-1 strawberry and non lransgenic strawberry administrated groups ;

轮状病毒是导致哺乳动物腹泻的最重要的病原体之一。我们已经生产了表达轮状病毒VP6 cDNA基因的转基因马铃薯植物，该基因编码外套蛋白。此外，我们已经证实了小鼠转基因马铃薯的抗原性。在这项研究中，我们生产了引入人干扰素（HIFN）基因的转基因草莓。另外，我们使用共转化技术生产了表达牛IL-1和轮状病毒VP6蛋白的转基因草莓。在这里，我们使用上述转基因草莓和/或上述转基因VP6马铃薯对口服给药的作用。BALBB/C（8周大）的小鼠一周一次与转基因植物自发喂食。最后一次（第3）施用1周后，检查了这些小鼠使用轮状病毒EW菌株（鼠A组），剂量为10^5 TCID50。病毒抗原和抗病毒抗体的血液和挑战小鼠的粪便进行了挑战。在VP6管理小鼠的粪便中检测到分泌的IgA。另一方面，HLFN草莓，IL-1草莓和非Lransgenic Strawberry管理组的免疫标记没有显着差异。

项目成果

Increase of antibody titer against Leucocytozoon caulleryi by oral administration of recombinant R7 antigen

• DOI: 10.1292/jvms.67.211
• 发表时间: 2005-02-01
• 期刊: JOURNAL OF VETERINARY MEDICAL SCIENCE
• 影响因子: 1.2
• 作者: Ito, A;Gotanda, T;Matsumura, T
• 通讯作者: Matsumura, T

Increase of Antibody Titer against Leucocytozoon caulleryj by Oral Administration of Recombinant R7 Antigen.

口服重组 R7 抗原可增加抗白细胞虫的抗体滴度。

• 发表时间: 2005
• 期刊: J.Vet.Med.Sci 67(2)
• 作者: Akira Ito;Tohru Gotand;Shigeki Kobayashi;Katsumi Kume;Chihiro Sugimoto;Takeshi Matsumura
• 通讯作者: Takeshi Matsumura

Ability of Orally Administered IFN-α containing Transgenic Potato Extracts to Inhibit Ldsteria monocytogenes Infection.

口服含有 IFN-α 的转基因马铃薯提取物抑制单核细胞增生李斯特氏菌感染的能力。

• 期刊: Journal of Interferon & Cytokine Research (In press)
• 作者: Kenji Ohya;Takeshi Matsumura.Noriko Itchoda;Kazuhiko Ohashi;Misao Onuma;Chiro Sugimoto
• 通讯作者: Chiro Sugimoto

Mammlian Cytokines Produced in pints.

哺乳动物细胞因子 以品脱量生产。

• 发表时间: 2004
• 期刊: Encyclopedia of Plant and Crop Science (MRCEL DEKKER, Inc) (In press)
• 作者: Chihiro Sugimoto;Takeshi Matsumura
• 通讯作者: Takeshi Matsumura