Development of measurement system of function analysis of membrane using low-coherence interferometry

低相干干涉膜功能分析测量系统的研制

• 批准号: 11555016
• 负责人: HARUNA Masamitsu
• 金额: $ 5.95万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11555016/
• 关键词: Low-coherence interferometry; Membrane; Phase transition; Phospholipid bilayers; Rat mesentery; in vivo measurement; Optical coherence tomography; 生体膜(腸間膜); 屈折率測定

Through this research project, we have developed in vivo measurement method for function analysis of membrane, using the low-coherence interferometery. The developed measurement system was used for detection of phase transition of both artificial membrane(DPPC)and rat mesentery. In particular, influence of circumstances of membrane on the phase transition was examined in detail ; as a result, it was found that the phase transition was closely related to the molecular structure of phospholipid bilayers.(1)In our developed system, refractive index and thickness of membrane are measured with an accuracy of 0.1% and 0.1μm, respectively, for detection of phase transition, using the low coherence interferometery. The transmission of membrane changes drastically by phase transition.(2)In the experiment, DPPC and rat mesentery were immersed in buffer solution, and the phase transition was detected by changing pH of buffer solution. In both DPPC and rat mesentery, it was found that the temperat … More ure for the main phase transition became higher as pH decreases. This result indicates that rat mesentery mainly consists of phospholipid bilayers.(3)In the case where ethanol is added to DPPC, the sub phase transition disappears for ethanol concentration of >25mg/ml. This drastic change for phase transition is due to the fact that phospholipid bilayers transforms to an inter digital structure.(4)By addition of surface active agent(palmitic acid), phospholipid bilayers transforms to micelle ; therefore, the normal phase transition disappears completely.(5)On the other hand, phase transition of rat mesentery is not influenced by addition of ethanol and surface active agent. This results suggests that protein and collagen disturb transformation of molecular structure of rat mesentery ; in other words, phospholipid bilayers are maintained in rat mesentery even in the presence of ethanol and surface active agent.(6)In order for in vivo measurement, a tomography image of a piece of rat mesentery was demonstrated, using the ultra-low coherence interferometry, which ere the resolution is only 1.3μm.On the basis of the experimental results, as described above, we are planning to develop a practical measurement system used in the field of physiology. Less

通过该研究项目，我们使用低稳态干扰开发了用于膜功能分析的体内测量方法。开发的测量系统用于检测人造膜（DPPC）和大鼠肠系膜的相变。特别是，详细研究了膜环境对相过渡的影响；结果，发现相变与磷脂双层的分子结构密切相关。（1）在我们开发的系统中，使用低相干性干扰的相位转换，以0.1％和0.1μm的精度测量了折射率和膜厚度。 （2）在实验中，DPPC和大鼠肠系膜浸入缓冲溶液中，并通过更改缓冲液溶液的pH值检测到相变。在DPPC和大鼠肠系膜中，发现随着pH的降低，主相变的温度更高。该结果表明大鼠肠系膜主要由磷脂双层组成。（3）在将乙醇添加到DPPC的情况下，乙醇浓度> 25mg/mL的乙醇浓度消失。相变的这种急剧变化是由于磷脂双层转化为数字结构的事实。（4）通过添加表面活性剂（棕榈酸），磷脂双层转化为胶束；因此，正常的相跃迁完全消失。（5）另一方面，大鼠肠系膜的相变不受添加乙醇和表面活性剂的影响。该结果表明，大鼠肠系膜分子结构的蛋白质和胶原蛋白灾难转化。换句话说，即使在存在乙醇和表面活性剂的情况下，也将磷脂双层保持在大鼠的肠系膜中。（6）为了进行体内测量，证明了一块大鼠肠系膜的层析成像图像，使用超低相干性干扰，这是对实验的依据，并且在实验的基础上进行了练习，我们的效果是练习的，我们的效果是在效果上进行了练习。生理领域。较少的

项目成果

Masamitsu Haruna: "In situ tomographic observation of tissue surface during laser ablation"Proc.SPIE, Progress in Biomed.Opt.& Imaging. Vol.425(to be published). (2001)

Masamitsu Haruna：“激光消融过程中组织表面的原位断层扫描观察”Proc.SPIE，Progress in Biomed.Opt。

Masamitsu Haruna: "Optical reflection imaging for display of 2-D refractive index distribution along the geometrical thickness"OSA Biomed.Topical Meetings,Tech.Digest. MB5. 194-196 (2000)

Masamitsu Haruna：“用于显示沿几何厚度的二维折射率分布的光学反射成像”OSA Biomed.Topical Meetings，Tech.Digest。

Masamitsu Haruna: "Optical Coherence Tomography(OTC), -Measurement priciple and development-"Gastrointestinal clinic. Vol.16, No.1. 131-133 (2001)

Masamitsu Haruna：“光学相干断层扫描（OTC），-测量原理和发展-”胃肠道诊所。

Hideki Maruyama: "A practical measurement system for determination of refractive index and thickness using the low coherence interferometry"1999 Int'l Conf.Opt.Eng.for Sensing and Nanotech.(ICOSN'99). Vol.3740. 26-29 (1999)

Hideki Maruyama：“使用低相干干涉测量法确定折射率和厚度的实用测量系统”1999 Intl Conf.Opt.Eng.for Sensing and Nanotech.(ICOSN99)。

近江 雅人: "低コヒーレンス光干渉による生体組織の屈折率と厚さ測定"医用電子と生体工学. Vol.37,No.1. 78-84 (1999)

Masato Omi：“通过低相干光学干涉测量生物组织的折射率和厚度”《医疗电子和生物工程》第 37 卷，第 78-84 期（1999 年）。