Research on simultaneous measurement of refractive index and thickness of bilogical tissue by low coherence interferometry

低相干干涉法同时测量生物组织折射率和厚度的研究

基本信息

批准号：
08455039
负责人：
HARUNA Masamitsu
金额：
$ 4.54万
依托单位：
Osaka University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

项目摘要

The research has been made to demonstrated a novel low coherence interferometer which can provide simultaneous measurement of the refractive index and thickness of biological tissue. Major research results are described in the following.1. We proposed and demonstrated a novel technique for simultaneous measurement of refractive index n and thickness t of a measured object. The technique is based on the low coherence interferometry combined with precise translation stages. It is applicable for accurate measurement of refractive index of biological tissue. The measurement accuracy of <0.1% is expected for a thickness of more than 1mm. In the experiment using the object scanning method, the accuracy of 0.2% or less was attained for nearly 1-mm thick transparent plates.2. On the basis of our proposed method, refractive index and thickness measurement were successfully achieved for a human nail, tooth, chicken tissue and a crustacean which has multiple layrs.3. We suggest that this method is applicable for birefringence measurement without any polarization control. The ordinary and extraordinary indices, n_o and n_e of a X-cut LiNbO_3 plate were measured.4. Moreover, we propose and demonstrate a novel technique for simultaneous measurement of plase and group lidices n_p and n_g of a transparent plate, as well as the thickness t, using the low coherence interferometer where a single superluminescent diode is used as the light source.5. To realize a stable and compact measurement system, the low coherence interferometer consisted of micro-optic components on a breadboard of 50 x 50cm^2.

该研究展示了一种新型低相干干涉仪，可以同时测量生物组织的折射率和厚度。主要研究成果如下： 1．我们提出并演示了一种同时测量被测物体的折射率 n 和厚度 t 的新技术。该技术基于低相干干涉测量与精确平移台相结合。适用于生物组织折射率的精确测量。对于厚度超过 1mm 的测量精度预计 <0.1%。采用物体扫描法进行的实验中，对于近1mm厚的透明板，精度达到了0.2%以下。 2．基于我们提出的方法，成功地实现了人类指甲、牙齿、鸡组织和多层甲壳动物的折射率和厚度测量。 3．我们建议该方法适用于无需任何偏振控制的双折射测量。测量了X切LiNbO_3板的普通指数和非常指数n_o和n_e。 4．此外，我们提出并演示了一种新技术，使用低相干干涉仪（其中单个超发光二极管用作光源）同时测量透明板的平面和群激光 n_p 和 n_g 以及厚度 t。 5 。为了实现稳定且紧凑的测量系统，低相干干涉仪由位于 50 x 50cm^2 面包板上的微光学元件组成。