Measurement of the neural activation potential of rat cerebral cortex using functional optical reflection tomography

功能性光学反射断层扫描测量大鼠大脑皮层神经激活电位

基本信息

  • 批准号:
    13480293
  • 负责人:
  • 金额:
    $ 5.95万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
  • 财政年份:
    2001
  • 资助国家:
    日本
  • 起止时间:
    2001 至 2002
  • 项目状态:
    已结题

项目摘要

Our research target is proposal and demonstration of a novel functional optical reflection tomography (f-ORT). This unique technique is used for measurement of spatial and temporal distribution of neural activation potential of the cerebral cortex of rats, showing that the f-ORT is effective for brain neurology.(1) A SLD is used as the light source of the existing optical coherence tomography (OCT); however, the coherence length of SLD is 10 to 20 μm which is insufficient for detection of neural activation potential. In order for the detection, the SLD was replaced by a mode-locked Ti:sapphire laser which supplied the pulsewidth as short as lOfs. This laser can provide us OCT with the spatial resolution of 3 μm after proper compensation of chromatic dispersion of the interferometer.(2) In order to the high-resolution OCX described above, we developed the 1,3-μm OCT in which the imaging depth is improved to be 1.5mm or more. Subsequently, the desired optical reflection tomography was de … More veloped by combination of the coherence-gate optics and confocal optics.(3) The imaging data must be acquired as fast as possible in order to detect the neural activation potential in synchronization with the cardiac rate. Such a high-speed data acquisition can be realized by non-mechanical optical-delay scanning. In the f-ORT, the optical-delay scanning is performed by PZT fiber phase modulators, as a result, more than 50 optical-delay scans per second were obtained. All the raster scan signals are summed up to improve drastically the signal-to-noise ratio.(4) In the preliminary experiment, a refractive index change was detected by the f-ORT when a sound stimulus was applied to a rat. The index change may correspond to the neural activation potential due to a sound stimulus. A remarkable change of the optical reflection signal was detected in a 100-μm deep area 20s after application of the sound stimulus. On the basis of research results, as mentioned above, we have been developing the f-ORT for brain neurology. Less
我们提出了我们的研究目标,并证明了新型功能性光学反射断层扫描(F-ORT)。这种独特的技术用于测量大鼠脑皮质的神经元激活潜力的空间和临时分布,表明F-ort对脑神经病学有效。但是,SLD的相干长度为10至20μM,不足以检测神经活化电位。为了进行检测,SLD被模式锁定的Ti:蓝宝石激光器代替,该蓝宝石激光器提供的脉冲宽与LOF一样短。该激光器可以在正确补偿干涉仪的色散后,为我们提供3μM的空间分辨率。(2)为了在上述高分辨率OCX时,我们开发了1,3-μmOCT,其中将成像深度改进为1.5mm或更多。随后,所需的光学反射断层扫描是DE…通过相干门口光学和共聚焦光学元件的组合更加恢复。这样的高速数据采集可以通过非机械光学 - 延迟扫描来实现。在F-ort中,光学 - 延迟扫描是通过PZT光纤相调节器进行的,因此,获得了每秒50多次光学 - 延迟扫描。所有的栅格扫描信号都是夏季,以极大地提高信号噪声比率。(4)在初步实验中,当将声刺激应用于大鼠时,F-ort检测到了折射率的变化。指数变化可能对应于由于声刺激引起的中性激活潜力。使用声刺激后,在20s深100μm的区域中检测到光学反射信号的显着变化。如上所述,根据研究结果,我们一直在开发大脑神经病学的F-ort。较少的

项目成果

期刊论文数量(112)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
春名 正光: "低コヒーレンス光干渉を用いた生体計測と断層イメージング"応用光学. Vol.3. 1-6 (2003)
Masamitsu Haruna:“使用低相干光学干涉的生物测量和断层成像”《应用光学》第 3 卷(2003 年)。
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    0
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春名 正光: "光コヒーレンストモグラフィ(OCT)"O plur E. Vol.24. 168-171 (2002)
Masamitsu Haruna:“光学相干断层扫描(OCT)”O plur E. Vol.24(2002)。
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    0
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Hideki Maruyama: "Low-coherence interferometer system for the simultaneous measurement of refractive index and thickness"Applied Optics. Vol.41No.7. 1315-1322 (2002)
Hideki Maruyama:“用于同时测量折射率和厚度的低相干干涉仪系统”应用光学。
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    0
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Masamitsu Haruna: "Novel low-coherence interferometric technique in biomedical measurement and imaging"Joint Symposium on Bio-Sensing and Bio-Imaging. Technical Digest. 140-145 (2001)
Masamitsu Haruna:“生物医学测量和成像中的新型低相干干涉技术”生物传感和生物成像联合研讨会。
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    0
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春名 正光: "光コヒーレンストモグラフィ(OCT)"O plus E. Vol.24No.2. 167-171 (2002)
Masamitsu Haruna:“光学相干断层扫描(OCT)”O plus E. Vol.24No.2(2002)。
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    0
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HARUNA Masamitsu其他文献

HARUNA Masamitsu的其他文献

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{{ truncateString('HARUNA Masamitsu', 18)}}的其他基金

Dynamic function analysis of skin sympathetic nerve using optical coherence tomography and its application to clinical diagnoses
光学相干断层扫描皮肤交感神经动态功能分析及其在临床诊断中的应用
  • 批准号:
    19300159
  • 财政年份:
    2007
  • 资助金额:
    $ 5.95万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Development of the high-resolution optical tomography capable for in vivo observation of biological tissue structure and the application to early-stage diagnosis of superficial cancer
开发可活体观察生物组织结构的高分辨率光学断层扫描技术及其在浅表性癌症早期诊断中的应用
  • 批准号:
    15300162
  • 财政年份:
    2003
  • 资助金额:
    $ 5.95万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Detection of calcium by the nanosecond time-gated spectroscopy of plume in laser ablation of biological tissue and its application to clinical diagnoses
生物组织激光消融中羽流纳秒时间选通光谱检测钙及其在临床诊断中的应用
  • 批准号:
    11480256
  • 财政年份:
    1999
  • 资助金额:
    $ 5.95万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B).
Development of measurement system of function analysis of membrane using low-coherence interferometry
低相干干涉膜功能分析测量系统的研制
  • 批准号:
    11555016
  • 财政年份:
    1999
  • 资助金额:
    $ 5.95万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B).
Research on simultaneous measurement of the phase and group indices and the thickness of transparent plates and the scattering medium by the low coherence interferometry
低相干干涉法同时测量透明板和散射介质的相位指数、群指数和厚度的研究
  • 批准号:
    09555009
  • 财政年份:
    1997
  • 资助金额:
    $ 5.95万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Research on simultaneous measurement of refractive index and thickness of bilogical tissue by low coherence interferometry
低相干干涉法同时测量生物组织折射率和厚度的研究
  • 批准号:
    08455039
  • 财政年份:
    1996
  • 资助金额:
    $ 5.95万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
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