Establishment of cementblast cell lines and periodontal ligament cell lines

成牙骨质细胞系和牙周膜细胞系的建立

基本信息

批准号：
11470378
负责人：
TAKATA Takashi
金额：
$ 4.67万
依托单位：
HIROSHIMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-11470378/
关键词：
cementoblast periodontal ligament cell cell line SV-40T hTERT gene transfection periodontal tissue 歯周靭帯細胞 SV-40 T-Ag 石灰化関連因子セメント質形成性線維腫 SV40T-Ag 石灰化関連蛋白不死化遺伝子 SV40 歯周疾患

项目摘要

The aim of the study was to establish the cell lines of periodontal ligament (PDL) fibroblasts and cementoblasts for elucidating cellular proliferation and differentiation of PDL. First, we developed the sequential enzymatic digestion method for isolation of regional cell populations (cementoblasts, precementoblast, PDL fibroblasts and undifferentiated cells) from rat molar PDL to demonstrate the heterogeneity of PDL constitutive cells. Next, we transfected immortality-related genes such as SV-40T and hTERT genes into the cell populations to establish their cell lines.1. We isolated cell populations with different growth potential and differentiation from rat and human PDL by sequential enzymatic digestion.2. Cementblast cell lines and PDL fibroblast cell lines were obtained from rat molar PDL cell subpopulation by the SV-40T transfection.3. We established human cementoblast cell lines and PDL fibroblast cell lines by the hTERT gene transfection into the regional cell populations.4. Human PDL cell lines were established from a human cementifying fibroma by the SV-40T and hTERT genes transfection.We established human and rat cementoblast cell lines and PDL fibroblast cell lines that can be widely provided for researchers. It is expected that these cell lines can be the novel in vitro cell model to study the regulatory mechanism of differentiation and proliferation of PDL and related studies including periodontal tissue regeneration, dental implant and tooth transplantation.

本研究的目的是建立牙周膜（PDL）成纤维细胞和成牙骨质细胞的细胞系，以阐明 PDL 的细胞增殖和分化。首先，我们开发了连续酶消化方法，用于从大鼠磨牙 PDL 中分离区域细胞群（成牙骨质细胞、前成牙骨质细胞、PDL 成纤维细胞和未分化细胞），以证明 PDL 组成细胞的异质性。接下来，我们将SV-40T和hTERT基因等永生相关基因转染到细胞群中，建立细胞系。1．我们通过连续酶消化从大鼠和人PDL中分离出具有不同生长潜力和分化能力的细胞群。2．通过SV-40T转染大鼠磨牙PDL细胞亚群，获得成牙骨质细胞系和PDL成纤维细胞系。 3．通过hTERT基因转染区域细胞群，建立了人成牙骨质细胞系和PDL成纤维细胞系。 4．人PDL细胞系是通过转染SV-40T和hTERT基因从人牙骨质纤维瘤中建立的。我们建立了人和大鼠成牙骨质细胞系和PDL成纤维细胞系，可广泛提供给研究人员。预计这些细胞系可以成为研究PDL分化和增殖调控机制以及牙周组织再生、牙种植和牙移植等相关研究的新型体外细胞模型。