Elucidation of invasive andmetastatic mechanism in oral cancer for application to molecular based diagnosisand gene therapy

阐明口腔癌的侵袭和转移机制，应用于分子诊断和基因治疗

• 批准号: 21249088
• 负责人: TAKATA Takashi
• 金额: $ 29.2万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21249088/
• 关键词: 口腔癌; 浸潤・転移; microRNA; 浸潤; 転移; 血管新生; 微小環境; MMP10; MMP13

To elucidate invasive and metastatic mechanism in oral cancer for application to molecular based diagnosis and gene therapy, we conducted research in accordance with our projects. As a result, we identified Wnt5B and MMP10 as promoter of , microRNA-203 as suppressor of tumor invasion, Periostin as promoter of lymphangiogenesis, MMP-13 as promoter of angiogenesis. Additionally, we attempted to apply Periostin to Elisa for serum of oral cancer patients. Interestingly, we found upregulation of Periostin correlated with lymphonode metastasis status. Therefore, we showed Periostin could be a useful for molecular diagnostic marker in oral cancer.

为了阐明口腔癌的侵袭和转移机制以应用于分子诊断和基因治疗，我们根据我们的项目进行了研究。结果，我们确定Wnt5B和MMP10为肿瘤侵袭的启动子，microRNA-203为肿瘤侵袭的抑制子，Periostin为淋巴管生成的启动子，MMP-13为血管生成的启动子。此外，我们尝试将Periostin应用于Elisa检测口腔癌患者的血清。有趣的是，我们发现骨膜素的上调与淋巴结转移状态相关。因此，我们表明 Periostin 可以作为口腔癌的分子诊断标记物。

项目成果

Matrix Metalloproteinase-13 (MMP-13) Directly and Indirectly Promotes Tumor Angiogenesis

• DOI: 10.1074/jbc.m112.373159
• 发表时间: 2012-11-09
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Kudo, Yasusei;Iizuka, Shinji;Takata, Takashi
• 通讯作者: Takata, Takashi

Cyclooxygenase-2 inhibition causes antiangiogenic effects on tumor endothelial and vascular progenitor cells

• DOI: 10.1002/ijc.25976
• 发表时间: 2012-01-01
• 期刊: INTERNATIONAL JOURNAL OF CANCER
• 影响因子: 6.4
• 作者: Muraki, Chikara;Ohga, Noritaka;Hida, Kyoko
• 通讯作者: Hida, Kyoko

RUNX3 has an oncogenic role in head and neck cancer.

• DOI: 10.1371/journal.pone.0005892
• 发表时间: 2009-06-12
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Tsunematsu T;Kudo Y;Iizuka S;Ogawa I;Fujita T;Kurihara H;Abiko Y;Takata T
• 通讯作者: Takata T

口腔癌の浸潤に関与する microRNA の網羅的解析

口腔癌侵袭相关microRNA的综合分析

• 发表时间: 2010
• 作者: 吉田真希;工藤保誠;高田 隆
• 通讯作者: 高田 隆

The 3D tissue microenvironment modulates DNA methylation and E-cadherin expression in squamous cell carcinoma

• DOI: 10.4161/epi.7.1.18546
• 发表时间: 2012-01-01
• 期刊: EPIGENETICS
• 影响因子: 3.7
• 作者: DesRochers, Teresa M.;Shamis, Yulia;Garlick, Jonathan A.
• 通讯作者: Garlick, Jonathan A.