Experimental study on periodontal tissue regeneration by transplantation of cultured periodontal ligament cells

培养牙周膜细胞移植再生牙周组织的实验研究

基本信息

批准号：
08672072
负责人：
TAKATA Takashi
金额：
$ 1.47万
依托单位：
HIROSHIMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

项目摘要

The aim of the present study was to elucidate whether new connective tissue attachment (CTA) is produced by transplantation of culture cells from periodontal ligament (PDL). We first discussed culture methods to obtain a large number of PDL cells for the transplantation and characterized the obtained PDL-derived culture cells. Subsequently, we establish an in vovo model in Lewis inbred strain rats for cell transplantation into periodontal defects. The following are the results obtained :1. The conventional 2-dimensional cultured method could supply enough amount of cells for transplantation into small periodontal defects in rats. It is also demonstrated that the floating culture system using collagen beads and spinner flasks provides more efficient growth of PDL-derived cells with inherent properties of PDL cells.2. Cell transplantation with atelocollagen membrane was evaluated as the easiest way to handle and to carry a large amount of cells.3. Transplantation of PDL-derived cells promoted bone regeneration and CTA formation.4. It was unexpectedly demonstrated that transplantation of bone marrow-derived osteoblastic cells promoted regeneration of bone and formation of CTA.5. Transplantation of gingiva fibroblasts showed less bone regeneration and CTA formation than controls where no cells were seeded.These results showed the possibilities to develop new therapeutic modalities for periodontal tissue regeneration with the transplantation of PDL-derived cells and bone marrow-derived osteoblastic (undifferentiated mesencymal) cells.

本研究的目的是阐明新的结缔组织附着（CTA）是否是通过移植牙周膜（PDL）培养细胞产生的。我们首先讨论了获得大量用于移植的 PDL 细胞的培养方法，并对获得的 PDL 衍生培养细胞进行了表征。随后，我们在Lewis近交系大鼠中建立了用于牙周缺损细胞移植的体内模型。得到的结果如下： 1．传统的二维培养方法可以提供足够数量的细胞移植到大鼠牙周小缺损中。还证明，使用胶原珠和旋转瓶的漂浮培养系统可以提供具有PDL细胞固有特性的PDL衍生细胞更有效的生长。2．去端肽胶原膜细胞移植被认为是处理和携带大量细胞的最简单方法。 3． PDL源细胞移植促进骨再生和CTA形成。 4．出人意料地证明，骨髓来源的成骨细胞的移植促进了骨的再生和CTA.5的形成。与未接种细胞的对照相比，牙龈成纤维细胞的移植显示出较少的骨再生和 CTA 形成。这些结果表明，通过移植 PDL 衍生细胞和骨髓衍生成骨细胞（未分化间质细胞）开发牙周组织再生新治疗方式的可能性）细胞。