NMR console (replacement) for 600 MHz spectrometer, cryogenic cooling platform and 19F probe head.

用于 600 MHz 光谱仪、低温冷却平台和 19F 探头的 NMR 控制台（替换件）。

• 批准号: 452632086
• 金额: --
• 依托单位: Goethe-Universität Frankfurt am Main
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2020
• 资助国家: 德国
• 起止时间: 2019-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/452632086?language=en
• 关键词: NMR; console; replacement; 600; MHz

The application is integrated into the project Covid19-NMR that started March 26th, 2020. In this international with more than 30 Partners from 10 countries, all viral RNAs and viral proteins of SARS-CoV-2 are produced and their NMR spectra analyzed and assignment. The biochemical production and the NMR-spectroscopic characterization (NMR assignments) forms the basis for a large screening project. The infrastructural support for this screening project is the topic of the current application. The aim of Covid19-NMR is to screen up to 20 viral proteins and 40 viral RNAs for binding of ligands and drugs. The low molecular weight compounds are assembled in five different libraries with up to 1000 ligands. Ligand-based screening is divided into ligands with 19F atoms and ligands without 19F. 19-based screening offers a number of advantages over 1H-based screening. Therefore, we apply for the upgrade of an existing 600 MHz-NMR-spectrometer with a 19F cryogenic probe allowing for 1H decoupling. To do so, we need to renew a 20 year old console and the associated cryogenic cooling unit. Evaluation of this applciation is urgent. The producing company Bruker, the sole company providing such eqiupment, has offered to deliver the equipment till August 2020 in order to expedite research in Covid19-NMR.

该应用程序已集成到 2020 年 3 月 26 日启动的 Covid19-NMR 项目中。在这个拥有来自 10 个国家的 30 多个合作伙伴的国际项目中，生产了 SARS-CoV-2 的所有病毒 RNA 和病毒蛋白，并对它们的 NMR 谱进行了分析和分配。生化生产和核磁共振波谱表征（核磁共振任务）构成了大型筛选项目的基础。该筛选项目的基础设施支持是当前申请的主题。 Covid19-NMR 的目的是筛选多达 20 种病毒蛋白和 40 种病毒 RNA，以了解配体和药物的结合情况。低分子量化合物组装在五个不同的库中，具有多达 1000 个配体。基于配体的筛选分为含有19F原子的配体和不含19F原子的配体。与基于 1H 的筛选相比，基于 19 的筛选具有许多优势。因此，我们申请升级现有的 600 MHz NMR 波谱仪，配备 19F 低温探头，以实现 1H 解耦。为此，我们需要更新一台已有 20 年历史的控制台和相关的低温冷却装置。对此应用程序的评估刻不容缓。生产公司布鲁克是唯一提供此类设备的公司，已提出在 2020 年 8 月之前交付设备，以加快 Covid19-NMR 的研究。