REQUEST TO PURCHASE A 600 MHZ NMR REPLACEMENT CONSOLE AND COLD PROBE

请求购买 600 MHZ NMR 替换控制台和冷探头

• 批准号: 8447885
• 负责人: JOSEPH J. H. ACKERMAN
• 金额: $ 43.67万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-08-01 至 2016-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8447885
• 关键词: Amyloid; Anesthesiology; Area; Biochemical; Biochemistry; Biological; Biology; Biomedical Engineering; Biomedical Research; Biophysics; Carbohydrates; Cardiology; Chemistry; Commit; Communicable Diseases; Communication; DNA Structure; Developmental Biology; Diet; Doctor of Philosophy; Funding; Health; Heart; Hour; Human; Internal Medicine; Light; Maintenance; Malignant Neoplasms; Metabolism; Molecular; Neurology; Noise; Ophthalmology; Pathology; Pathway interactions; Pattern; Pharmacology; Physics; Radiation; Radiology Specialty; Research; Resolution; Science; Seasons; Signal Transduction; Structure; Sum; Technology; Time; Tissues; Training; Universities; Washington; base; diabetic; improved; instrument; interest; operation; programs; relating to nervous system; research study

DESCRIPTION (provided by applicant): The Washington University High Resolution NMR Facility is requesting funds to purchase a state- of-the-art replacement console and a high-end technology cold probe for its core 600-MHz NMR instrument. The combination of new console and cold probe will markedly improve signal-to-noise and instrument stability, thus greatly facilitating ongoing biochemistry and biomedical research programs, ultimately impacting human health. This request will modernize the highest field spectrometer of the University-wide NMR Facility that serves 40 separate research groups, spanning departments of Anesthesiology, Biochemistry & Molecular Biophysics, Biology, Biomedical Engineering, Cardiology, Chemistry, Infectious Diseases, Internal Medicine, Neurology, Ophthalmology, Pathology, Pharmacology & Developmental Biology, Physics, and Radiology (Division of Radiation Sciences). The NMR Facility supports and maintains six instruments and is staffed with seasoned PhD-level professionals who are committed to user training and collaborative research. The Facility is in its 28th year of operation, is open and available 24 hours a day, 365 days a year, and is committed to serving its extensive user base. This funding request is prompted by the need to maintain the Facility's capabilities in the face of extraordinary technological advances over the past decade, to reduce instrument down-time and maintenance expense, and importantly, to add an ultra-high-sensitivity cold probe that will enable new experiments and provide experimental efficiencies via significantly enhanced signal sensitivity. The scientific research described in ths proposal spans user groups with longstanding interests in fundamental biological, biochemical, and biomedical research and clinically related health questions. These including research areas directed toward light initiated alterations of DNA structure, the creation of molecular patterns that mimic neural communication networks, understanding markers for the genesis and recognition of cancer, understanding structure and genesis of amyloid precursors, elucidation of tissue damage pathways caused by reactive oxidative molecules, and deciphering the altered metabolism of the diabetic heart as well as metabolism alterations associated with carbohydrate-poor diet. This request is made with a financial matching fund commitment from Washington University in the sum of $195,000.

描述（由申请人提供）：华盛顿大学高分辨率 NMR 设施正在申请资金为其核心 600 MHz NMR 仪器购买最先进的替换控制台和高端技术冷探头。新型控制台和冷探头的结合将显着提高信噪比和仪器稳定性，从而极大地促进正在进行的生物化学和生物医学研究项目，最终影响人类健康。这一要求将对全校 NMR 设施的最高场谱仪进行现代化改造，该设施为 40 个独立的研究小组提供服务，涵盖麻醉学、生物化学和分子生物物理学、生物学、生物医学工程、心脏病学、化学、传染病、内科、神经病学、眼科等部门、病理学、药理学和发育生物学、物理学和放射学（放射科学部）。 NMR 设施支持和维护六台仪器，并配备了经验丰富的博士级专业人员，他们致力于用户培训和协作研究。该设施位于其 运营第 28 年，全年 365 天、每天 24 小时开放，致力于服务广大用户群。提出这一资助请求的原因是，在过去十年中，面对非凡的技术进步，需要保持该设施的能力，减少仪器停机时间和维护费用，重要的是，增加一个超高灵敏度的冷探头，它将通过显着增强的信号灵敏度实现新的实验并提高实验效率。该提案中描述的科学研究涵盖了对基础生物学、生化和生物医学研究以及临床相关健康问题长期感兴趣的用户群体。这些研究领域包括光引发的 DNA 结构改变、模拟神经通讯网络的分子模式的创建、了解癌症发生和识别的标记、了解淀粉样蛋白前体的结构和发生、阐明反应引起的组织损伤途径。氧化分子，并破译糖尿病心脏代谢的改变以及与碳水化合物缺乏饮食相关的代谢改变。这一请求是在华盛顿大学承诺提供 195,000 美元的财务配套资金的情况下提出的。