MONITORING OF MUTAGENICITY IN AQUATIC BIOTA AS A TECHNIQUE FOR THE ENVIRONMENTAL ASSESSMENT

作为环境评估技术的水生生物群突变性监测

基本信息

批准号：
03454207
负责人：
KIRA Shohei
金额：
$ 4.1万
依托单位：
OKAYAMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1991
资助国家：
日本
起止时间：
1991 至 1992
项目状态：
已结题

项目摘要

Mussels were harvested at 5 selected sites along the coast of Seto Inland Sea and their edible parts were extracted with acetone-acetonitrile. The extract was then subjected to blue rayon (BR) treatment and the sample thus prepared was analyzed for mutagenicity with Salmonella typhimurium YG1024 and for benzo(a)pyrene (BaP) with HPLC-Fl. Eight out of 49 mussel groups contained BaP more than 1.0 ng/g and 17 out of 29 group showed positive results, the number of revertants exceeded 3 times more than that of solvent control. A positive correlation was observed between the level of BaP and the mutagenicity in individual groups. Blue rayon was hunged for 24 hr in the sea water at the same sites where mussels were collected. The eluate with methanoammonia was condensed and also assayed mutagenicity and BaP content. The eluate from 1g equivalent BR contained 0.05 to 19.5 ng of BaP and 17 out of 29 samples showed positive results. Again a positive correlation was observed between the amount of BaP and the mutagenicity. In order to identify the attributable components to the mutagenicity, we fractionated sample extract from mussel and the eluate from BR through C18 column with HPLC and assayed mutagenicity in each fraction. In case of mussel, elevated mutagenicity was observed in the polar fraction than that contained BaP. Further Yamato-shijimi, Corbicula japonica, was experimentally exposed to BaP at 0.058-0.224 ng/ml in an aquarium. After 12 and 24 hr, an elevated mutagenicity was again observed in the polar fraction, however the bivalve accumulated merely 11-38 times higher level of BaP than that in the aquarium. The above results suggest that a monitoring of mutagenicity in biota samples will provide a potential approach to carcinogenic substances in the aquatic environment.

在塞多内陆海岸的5个选定地点收获贻贝，并用丙酮 - 乙腈提取其可食用的部分。然后对提取物进行蓝色人造丝（BR）处理，并分析了这样制备的样品，以用伤寒沙门氏菌YG1024和苯并（A）pyrene（BAP）用HPLC-FL分析诱变。在49个贻贝组中，有8个包含BAP超过1.0 ng/g，而29组中有17个显示出阳性结果，恢复体的数量比溶剂对照的比例高3倍。单个组的BAP水平与诱变水平之间观察到正相关。在收集贻贝的同一地点，在海水中，蓝色人造丝在海水中被饥饿24小时。用甲烷瘤的洗脱器被凝结，还测定了诱变性和bap含量。从1G等效的BR含有0.05至19.5 ng的BAP，29个样品中有17个显示阳性结果。同样，在BAP和诱变性之间观察到正相关。为了识别诱变性的可归因成分，我们从贻贝中分离了样品提取物，并从BR到C18柱中的洗脱液中用HPLC进行了hplc，并在每个馏分中分析了诱变。在贻贝的情况下，在极性分数中观察到较高的诱变性高于包含BAP的诱变性。在水族馆中，Yamato-shijimi（Corbicula Japonica）在实验中以0.058-0.224 ng/ml的速度暴露于BAP。在12和24小时后，在极性分数中再次观察到较高的诱变级，但是双壳类的BAP水平仅比水族馆中的BAP高11-38倍。以上结果表明，对生物群样品中诱变性的监测将为水生环境中的致癌物质提供潜在的方法。