Development of a method for measurement of a time-weighted average concentration of polycyclic mutagens in marine environment-for practiced utilization in monitoring of marine environment.

开发一种测量海洋环境中多环诱变剂时间加权平均浓度的方法 - 用于海洋环境监测的实际应用。

基本信息

批准号：
11557030
负责人：
KIRA Shohei
金额：
$ 5.82万
依托单位：
OKAYAMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

项目摘要

(1) Results of measuring the time-weighted average (TWA) concentration of Benzo (a) pyrene (BaP) in the Seto Inland Sea, Japan.The values of the TWA concentration of BaP from 1999 to 2000 ranged from 0.16 to 8.77 ng/L for the sampling sites in Okayama seashore, from 0.08 to 7.04 ng/L for Osaka Bay sites and from undetectable level (0.01 ng/L) to 18.88 ng/L for the sites in Northern Kyushu.(2) Effects of water temperature on the decrease in weight of plaster balls.We gave a dissolving test by hanging a plaster ball in moving water and found that the decrease in weight of the plaster ball correlated well with water flow intensity, and the regression coefficient and the intercept of regression line were affected by water temperature. We obtained the following equation :C={ΔW-(0.1232T+0.2247)}/(0.0189T+0.8162)where C is water flow intensity (cm/sec), ΔW is the decrease in weight of plaster ball (g) and T is water temperature (℃) and Therefore, according to this equation, it becomes possibl … More e to correct the value of measured TWA if water temperature is known.(3) Preserving condition of blue rayon after hanging and the recovery rate of BaP.One nanogram of BaP was dissolved in distilled water in a beaker and let BaP adsorbed to BR.We then calculated the recovery rate from amount of BaP after keeping it under various conditions against the initial one that was obtained from extraction without preservation. They were 101.6±8.2% (for one week) and 100.0±6.8% (for two weeks) when BR was preserved with aluminium-foil-wrapping at -5℃, and 93.5±5.1% (for four weeks) when preserved with transparent-plastic-foil-wrapping at 15-2O℃. The amount of the BaP adsorbed to BR has maintained over 90% even in the room temperature. Our study provides a convenient and a practical procedure for long-distance transportation of the samples.(4) Relationship between the amount of BaP and mutagenicity in samples after BR hanging.We have reported that mutagenicity detected by fhe BR hanging technique positively correlated with the amount of BaP.The present study unexpectedly found some samples despite of small amount of BaP showed high mutagenicity. Most of these phenomena were observed on the samples hanging in estuaries of the rivers flowing through the cities. This fact suggests that the city sewages might contain highly mutagenic substances except for BaP.(5) Publications and presentation of the study.We have published and presented the study results in scientific journals and at some conferences (see references). Furthermore, we are preparing for presentations at following international symposiums in order to receive various evaluations from foreign scientists : (1) PRIMO11 (July 2001, Plymouth, UK), (2) ISTA10 (August 2001, Quebec, Canada), (3) 8thICEM (October 2001, Shizuoka, Japan). Less

（1）测量日本Seto Inline Sea中时间加权平均（TWA）pyrene（BAP）的结果。1999年至2000年BAP的值范围为0.16至8.77 ng /L海滨，大阪湾站点的0.04 ng/l从不确定的（0.01 ng/l）到北九州北部的地点（2）水温的效果对灰泥降低的效果。水流量强度，而通道系数coeffffffffffffffffffffffffffffffffffffffficiont和sompertect and sompertect of Sermestect均受水温的影响。 sec），ΔW是石膏球（G）和I水温（°C）的重量减少，因此，Accomes方程将成为可能的……如果已知水温度，则更纠正测量值的值。（3）在悬挂式悬挂率和bap的恢复速率的情况下，将其固定在各种条件下，而不是在没有保存的情况下获得的。在-5°C时±6.8％，当在15-2O的透明塑料包裹中保存时，±5.1％（持续四个星期），即使在房间中，BAP吸附的BAP的量也保持在90％以上。我们的研究提供了一种方便的，用于样品的长距离运输诱变的高度表明，城市可能包含高度的诱变物质。（1）Primo11（2001年7月（2001年7月），英国普利茅斯），（2）ISTA10（2001年8月，加拿大魁北克），（3）8thicem（2001年10月，日本Shizuoka）