AN IMPROVED PROCEDURE FOR SAMPLING MUTAGENS BY HANGING BLUE RAYON IN AQUATIC ENVIRONMENT

水生环境中悬挂蓝色人造丝诱变剂取样的改进方法

• 批准号: 06670380
• 负责人: KIRA Shohei
• 金额: $ 1.15万
• 依托单位: OKAYAMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670380/
• 关键词: AQUATIC ENVIRONMENT; MUTAGENICITY; MONITORING; POLYCYCLIC AROMATICS; INTENCITY OF WATER STREAM; GYPSUM BALL; CORRECTION; BLUE RAYON; 石コウ球; 変異原性物質; 多環芳香族炭化水素; Benzo(a)pyrene; 時間平均値; AQUATIC ENVIRONMENT; MUTAGENICITY; MONITORING; POLYCYCLIC AROMATICS; INTENCITY OF WATER STREAM; GYPSUM BALL; CORRECTION; BLUE RAYON; 石コウ球; 変異原性物質; 多環芳香族炭化水素; Benzo(a)pyrene; 時間平均値

For monitoring polycyclic mutagens in water, we proposed a sampling technique : hanging blue rayon (an adsorbent selective for polycyclic compund) in water sites. The amount measured by this technique may be affected by water movement during hanging, although the procedure is simple and informative. Here, we have improved this technique more quantitatively, through correcting the amount by the intensity of water stream.(1) We used benzo (a) pyrene (BaP) as a model for polycyclic mutagen. Studies in an experimental aquarium showed a hanging blue rayon is an efficient monitor for BaP in water, however the amount adsorbed increases when the water is stirred vigorously.(2) We applied a gypsum-ball method proposed by Komatsu as a simple measurement for intensity of water movement. A reduced eight during a period of 24-hr hanging (Wd) correlated well with the average flow of water measured by an electromagnetic flow meter. Therefore a simple use of measured Wd for correcting the amount of Ba … More P is equivalent to the correction by the intensity when the procedure is accompanied by hanging blue rayon in water.(3) We set up a portable sampler for BaP in water, through immersed Sep-Pak C18 as an adsorbent, the water was continuously passed by a battery-operated pump. We could measure ng/L-level of BaP in environmental waters as the time-weighted average (TWA-BaP) by the sampler, however the apparatus is expensive.(4) We measured the amount of BaP adsorbed by blue rayon (BR-BaP) and Wd of gypsum-ball during similutaneous hanging. A corrected value calculated by deviding BR-BaP with Wd showed good correlation with TWA-BaP (correlation coefficients were 0.865 with correction and 0.818 without). The correlation equation given here was {(TWA-BaP)=6.5x(BR-BaP)/(Wd)}. The results suggest that the correction improves accuracy of measurement quantitatively.(5) We selected 2 sampling sites : one was a port surrounded by big industries and the other was far from the origin of pollution. Corrected values of water-borne BaP at the former site ranged from 0.14 to 0.63 ng/gBR/gGypsum and those at the latter site was less than 0.16. The results explain the former site was more polluted by BaP than the latter in contrast. Less

为了监测水中的多环子诱变剂，我们提出了一种采样技术：在水位上悬挂蓝色人造丝（用于多环芳烃的吸附剂选择性）。该技术测量的数量可能会受到悬挂期间水流动的影响，尽管该过程简单且内容丰富。在这里，我们通过通过水流的强度纠正数量来更加定量地改进了这一技术。（1）我们使用苯并（a）pyrene（bap）作为实验水族馆的研究表明，悬挂的蓝色人造丝是水中的有效监控器，但是当水的速度较慢时，我们在水中的bap有效。用于水的强度。在24小时悬挂期（WD）期间，减少了八个，与通过电磁流量计测得的水的平均水流很好地相关。因此，简单地使用测得的WD来纠正BA的量……更多的P等于当该过程伴随着蓝色人造裤悬挂在水中时的强度。（3）我们设置了一个通过沉浸在Sep-pak C18中的便携式采样器，用于sep-pak c18，作为一个吸附，水是通过packing powdered powdered powdered powdered powdered powdered powdered powdered powdered powdered powderaper powdered powdered powdered powdered powdered powdered powlaper-powdered。我们可以在环境水域中测量NG/L级BAP作为采样器的时间加权平均值（TWA-BAP），但是设备很昂贵。（4）我们测量了蓝色rayon（Br-bap）和在齐射悬挂期间Gypsum-Ball wd的Bap吸附的BAP量。通过用WD伸出BR-BAP计算的校正值与TWA-BAP显示良好的相关性（相关系数为0.865，校正为0.818，没有0.818）。此处给出的相关方程为{（twa-bap）= 6.5x（br-bap）/（wd）}。结果表明，校正可以定量地提高测量的准确性。（5）我们选择了2个采样地点：一个是一个被大型行业包围的端口，另一个端口远非污染的起源。以前位置的水传播BAP的校正值范围为0.14至0.63 ng/gbr/ggypsum，而后者位置的较为频率为0.16。结果解释了与后者相比，BAP比后者更受污染。较少的