AN IMPROVED PROCEDURE FOR SAMPLING MUTAGENS BY HANGING BLUE RAYON IN AQUATIC ENVIRONMENT

水生环境中悬挂蓝色人造丝诱变剂取样的改进方法

基本信息

批准号：
06670380
负责人：
KIRA Shohei
金额：
$ 1.15万
依托单位：
OKAYAMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06670380/
关键词：
AQUATIC ENVIRONMENT MUTAGENICITY MONITORING POLYCYCLIC AROMATICS INTENCITY OF WATER STREAM GYPSUM BALL CORRECTION BLUE RAYON 石コウ球変異原性物質多環芳香族炭化水素 Benzo(a)pyrene 時間平均値

项目摘要

For monitoring polycyclic mutagens in water, we proposed a sampling technique : hanging blue rayon (an adsorbent selective for polycyclic compund) in water sites. The amount measured by this technique may be affected by water movement during hanging, although the procedure is simple and informative. Here, we have improved this technique more quantitatively, through correcting the amount by the intensity of water stream.(1) We used benzo (a) pyrene (BaP) as a model for polycyclic mutagen. Studies in an experimental aquarium showed a hanging blue rayon is an efficient monitor for BaP in water, however the amount adsorbed increases when the water is stirred vigorously.(2) We applied a gypsum-ball method proposed by Komatsu as a simple measurement for intensity of water movement. A reduced eight during a period of 24-hr hanging (Wd) correlated well with the average flow of water measured by an electromagnetic flow meter. Therefore a simple use of measured Wd for correcting the amount of Ba … More P is equivalent to the correction by the intensity when the procedure is accompanied by hanging blue rayon in water.(3) We set up a portable sampler for BaP in water, through immersed Sep-Pak C18 as an adsorbent, the water was continuously passed by a battery-operated pump. We could measure ng/L-level of BaP in environmental waters as the time-weighted average (TWA-BaP) by the sampler, however the apparatus is expensive.(4) We measured the amount of BaP adsorbed by blue rayon (BR-BaP) and Wd of gypsum-ball during similutaneous hanging. A corrected value calculated by deviding BR-BaP with Wd showed good correlation with TWA-BaP (correlation coefficients were 0.865 with correction and 0.818 without). The correlation equation given here was {(TWA-BaP)=6.5x(BR-BaP)/(Wd)}. The results suggest that the correction improves accuracy of measurement quantitatively.(5) We selected 2 sampling sites : one was a port surrounded by big industries and the other was far from the origin of pollution. Corrected values of water-borne BaP at the former site ranged from 0.14 to 0.63 ng/gBR/gGypsum and those at the latter site was less than 0.16. The results explain the former site was more polluted by BaP than the latter in contrast. Less

为了监测水中的多环诱变剂，我们提出了一种采样技术：将蓝色人造丝（一种对多环化合物的选择性吸附剂）悬挂在水体中。尽管该方法简单且信息丰富，但所测量的量可能会受到悬挂技术过程中水流运动的影响。在这里，我们通过水流强度校正量，更定量地改进了这项技术。（1）我们在实验中使用苯并（a）芘（BaP）作为多环诱变剂研究的模型。水族馆表明，悬挂的蓝色人造丝可以有效监测水中的 BaP，但是，当水剧烈搅拌时，吸附量会增加。（2）我们应用小松提出的石膏球方法作为水运动强度的简单测量。 24 小时悬挂 (Wd) 期间减少的 8 与电磁流量计测量的平均水流量密切相关，因此，可以简单地使用测量的 Wd 来校正 Ba … 更多 P 的量。相当于该过程伴随着蓝色人造丝在水中的强度校正。(3)我们设置了水中BaP的便携式采样器，通过浸没的Sep-Pak C18作为吸附剂，使水连续通过一个过滤器。我们可以通过采样器测量环境水中的 ng/L BaP 水平作为时间加权平均值 (TWA-BaP)，但设备很昂贵。 (4) 我们测量了同时悬挂时蓝色人造丝（BR-BaP）吸附的BaP和石膏球的Wd用BR-BaP除以Wd计算出的校正值与TWA-BaP显示出良好的相关性（校正后相关系数为0.865，未校正时相关系数为0.818）。这里给出的相关方程为{(TWA-BaP)=6.5x(BR-BaP)/(Wd)}。结果表明，校正定量地提高了测量的准确性。(5)我们选择了2个采样点：一个是大型工业包围的港口，另一个是远离污染源的水载BaP校正值。前一个地点的范围为 0.14 至 0.63 ng/gBR/gGypsum，而后一个地点的值小于 0.16，结果说明前一个地点的 BaP 污染程度较后者少。