Biological monitoring of trace chemicals in residential environments

居住环境中微量化学物质的生物监测

基本信息

批准号：
13670365
负责人：
KIRA Shohei
金额：
$ 2.3万
依托单位：
Okayama University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

项目摘要

objective : Sick house syndrome, the diverse health effects encountered by people dwelling or working in the new construction, is becoming a noticeable problem in Japan. This study was attempted to make a contribution toward prediction of the adverse health effects of chemicals in residential environments and planning preventive measures. This year we focused on developing a method for quantitative determination of trace level metabolites in urine of individuals exposed to aromatic volatile organic compounds (VOCs) such as toluene, xylene, and styrene by gas chromatography coupled with mass spectrometry (GC-MS).Methods : We targeted of the urinary hippuric acid (HA), o-, m-, and p-methyl hippuric acids (o-, m-, p-MHA), and mandalic acid (MA) and phenylglyoxalic acid (PGA), metabolites of toluene, xylene, and styrene, respectively. Study samples were prepared by adding benzoylleucine as the internal standard (LS.), extracting with ethyl acetate under acid condition. The derivative compo … More unds were then reconstituted in ethyl acetate and identified by GC-MS equipped with DB-1 capillary column. Elution was carried out at initial column temperature 150℃ with an increment at a rate of 10℃/min up to 250℃. The quantitative analysis was operated in SIM mode, selecting the ion m/z 105 for HA, m/z 119 for MHA, m/z 107 for MA, and m/z 105 for PGA. In order to examine the corresponding concentrations of VOCs in ambient air, active sampling by charcoal tubes was used for VOCs collection. After elution with carbon disulfide, the samples were analyzed with GC-MS.Results and discussion : Six urinary metabolites and I.S. were separated and eluted with a good peak by GC-MS. The limits of detection were 40-450 pg by SIM mode and the calibration curves of these compounds were fairly linear. Hippuric acid, MHA, MA, and PGA were all detected from urine of workers working in the new buildings in which higher levels of VOCs (toluene, xylene, and styrene) were found. Since HA of dietary sources could also affect its background level in urine, it cannot become a urinary marker of exposure to trace toluene. However, MHA, MA, and PGA can be used to evaluate low-level exposure to xylene and styrene.Conclusions : This newly developed GC-MS method showed high sensitivity for determination of urinary metabolites of individuals exposed to trace level VOCs. The present results support its usefulness in biological monitoring of indoor VOCs for animals and humans. Less

目标：病房综合症，即在新建筑中居住或工作的人们所遇到的各种健康影响，正在成为日本的一个引人注目的问题。这项研究旨在为预测居住环境中化学品的不利健康影响做出贡献。今年，我们的重点是开发一种方法，通过气相色谱结合质谱法定量测定接触芳香族挥发性有机化合物 (VOC) 的个体尿液中的痕量代谢物。 (GC-MS)。方法：我们以尿马尿酸 (HA)、o-、m- 和 p-甲基马尿酸 (o-、m-、p-MHA) 和扁桃酸 (MA) 为目标，分别通过添加苯甲酰亮氨酸作为内标 (LS.) 来制备苯乙醛酸 (PGA)、甲苯、二甲苯和苯乙烯的代谢物。酸性条件下用乙酸乙酯萃取，然后用乙酸乙酯复溶，并通过配备DB-1毛细管柱的GC-MS进行鉴定，在初始柱温150℃下以一定速率递增。 10℃/min 至 250℃ 定量分析在 SIM 模式下进行，HA 选择离子 m/z 105， MHA 的 m/z 119，MA 的 m/z 107，PGA 的 m/z 105 为了检查环境空气中 VOC 的相应浓度，在用碳洗脱后，采用活性炭管进行 VOC 收集。结果和讨论：六种尿液代谢物和 I.S. 被分离并洗脱，具有良好的峰。 SIM 模式的 GC-MS 检测限为 40-450 pg，并且这些化合物的校准曲线均从在新建筑中工作的工人的尿液中检测到。发现了较高含量的挥发性有机化合物（甲苯、二甲苯和苯乙烯），因为膳食来源的 HA 也会影响其在尿液中的背景水平，因此它不能成为暴露于微量甲苯的尿液标志物。然而，MHA、MA 和 PGA 可用于评估二甲苯和苯乙烯的低水平暴露。结论：这种新开发的 GC-MS 方法对于测定暴露于痕量 VOC 的个体的尿液代谢物显示出高灵敏度。支持其在动物和人类室内 VOC 生物监测中的有用性。