Chemically Defined Subpopulations of GABAergic Neurons in the Cerebral cortex.

大脑皮层中 GABA 能神经元的化学定义亚群。

基本信息

批准号：
01480113
负责人：
KOSAKA Toshio
金额：
$ 3.84万
依托单位：
Kyushu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01480113/
关键词：
CNS cerebral cortex GABA Ca-binding protein monoclonal antibody immunocytochemistry stereology 免疫組織化学

项目摘要

Monoclinal antibodies HNK-1 and VC1.1 were shown to outline selectively a subpopulation of GABAergic neurons containing a specific calcium-binding protein parvalbumin (PV) in the adult rat parietal cortex, using pre- and post-embedding immunocytochemistry at light microscopic level. About 98% of HNK-1 stained cells in the rat parietal cortex were PV immunoreactive. About 95% of HNK-1 immunoreactive cells were also shown to be stained with a lectin, Vicia villosa agglutinin (VVA), with a specific affinity for terminal N-acetylgalactosamine, Previously shown to selectively stain a population of PV-containing GABAergic neurons in this region. Furthermore it was shown that almost all of HNK-1 immunoreactive cells were also stained with a monoclinal antibody 3B3, which has been established to be specific to chondroitin sulfate proteoglycan and was shown in the present study to selectively label a population of PV-immunoreative neurons in the adult rat parietal cortex. In addition, a direct comparison of two monoclonal antibodies HNK-1 and VC1.1 revealed that both were identical in their staining properties and that they defined the same subset of PV-containing GABAergic neurons in the rat rietal cortex. In the rodent cortex PV-containing neurons might be a distinctive subpopulation of GABAergic neurons, and, furthermore, specific subsets of cerebral PV-containing GABAergic neurons might be considered to have a particular microenvironment characterized by those molecular probes.

使用光镜水平上的包埋前和包埋后免疫细胞化学，显示单斜抗体 HNK-1 和 VC1.1 可选择性地勾勒出成年大鼠顶叶皮层中含有特定钙结合蛋白小清蛋白 (PV) 的 GABA 能神经元亚群。大鼠顶叶皮质中约 98% 的 HNK-1 染色细胞具有 PV 免疫反应性。大约 95% 的 HNK-1 免疫反应性细胞也被证明被一种凝集素、蚕豆凝集素 (VVA) 染色，该凝集素对末端 N-乙酰半乳糖胺具有特定的亲和力，此前显示可选择性地对一组含有 PV 的 GABA 能神经元进行染色。这个地区。此外，几乎所有 HNK-1 免疫反应细胞也被单斜抗体 3B3 染色，该抗体已被确定为硫酸软骨素蛋白多糖特异性，并且在本研究中显示可选择性标记 PV 免疫反应神经元群体在成年大鼠顶叶皮质中。此外，对两种单克隆抗体 HNK-1 和 VC1.1 的直接比较表明，两者的染色特性相同，并且它们在大鼠皮层中定义了相同的含有 PV 的 GABA 能神经元子集。在啮齿动物皮层中，含有PV的神经元可能是GABA能神经元的独特亚群，此外，含有PV的GABA能神经元的大脑特定亚群可能被认为具有以这些分子探针为特征的特定微环境。