Chemically Defined Subpopulations of GABAergic Neurons in the Cerebral cortex.

大脑皮层中 GABA 能神经元的化学定义亚群。

基本信息

批准号：
01480113
负责人：
KOSAKA Toshio
金额：
$ 3.84万
依托单位：
Kyushu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01480113/
关键词：
CNS cerebral cortex GABA Ca-binding protein monoclonal antibody immunocytochemistry stereology 免疫組織化学

项目摘要

Monoclinal antibodies HNK-1 and VC1.1 were shown to outline selectively a subpopulation of GABAergic neurons containing a specific calcium-binding protein parvalbumin (PV) in the adult rat parietal cortex, using pre- and post-embedding immunocytochemistry at light microscopic level. About 98% of HNK-1 stained cells in the rat parietal cortex were PV immunoreactive. About 95% of HNK-1 immunoreactive cells were also shown to be stained with a lectin, Vicia villosa agglutinin (VVA), with a specific affinity for terminal N-acetylgalactosamine, Previously shown to selectively stain a population of PV-containing GABAergic neurons in this region. Furthermore it was shown that almost all of HNK-1 immunoreactive cells were also stained with a monoclinal antibody 3B3, which has been established to be specific to chondroitin sulfate proteoglycan and was shown in the present study to selectively label a population of PV-immunoreative neurons in the adult rat parietal cortex. In addition, a direct comparison of two monoclonal antibodies HNK-1 and VC1.1 revealed that both were identical in their staining properties and that they defined the same subset of PV-containing GABAergic neurons in the rat rietal cortex. In the rodent cortex PV-containing neurons might be a distinctive subpopulation of GABAergic neurons, and, furthermore, specific subsets of cerebral PV-containing GABAergic neurons might be considered to have a particular microenvironment characterized by those molecular probes.

单阶抗体HNK-1和VC1.1被证明可以选择性地概述成年大鼠钙质皮质中含有特定钙结合蛋白白蛋白（PV）的GABA能神经元的亚群，使用前和后膜的膜皮层中的成年大鼠肾上腺皮质中的细胞构造，并在光显微显微镜水平上使用前和后膜。大鼠顶叶皮层中约98％的HNK-1染色细胞为PV免疫反应。还显示，大约95％的HNK-1免疫反应性细胞被凝集素，vicia villosa凝集素（VVA）染色，对末端N-乙酰乳糖胺具有特异性亲和力，先前显示为在该区域有选择性地染色含有PV的Gabagagic神经元。此外，还表明，几乎所有HNK-1免疫反应性细胞还用单斜抗体3B3染色，该细胞抗体3B3已确定为特定于硫酸软骨素硫酸软骨蛋白蛋白聚糖，并在本研究中显示，以选择性地将A pV-runimunoreative神经元在成人比率cortex cortex cortex cortex porietal cortex中标记。此外，两种单克隆抗体HNK-1和VC1.1的直接比较表明，两者在其染色特性中都是相同的，并且它们定义了大鼠Rietal Cortex中含有PV的GABA能神经元相同的子集。在啮齿动物皮质中，含有PV的神经元可能是GABA能神经元的独特亚群，此外，可以认为含有大脑PV的GABA能神经元的特定子集可能被认为具有由这些分子探针表征的特定微环境。