Cellular organization in the hippocampal formation

海马结构中的细胞组织

基本信息

批准号：
09480213
负责人：
KOSAKA Toshio
金额：
$ 8.51万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

项目摘要

Neuronal organization of the hippocampal formation is traditionally based on the neuronal types defined by classical morphological method such as Golgi impregnation. However recent progress in immunocytochemistry and other methods revealing chemical nature of neuronal elements makes it possible to define chemical subpopulations of neurons, which are not necessarily corresponding to classical morphologically defined neuronal types. In the present project we are intending combined light and electron microscopic analyses of immunocytochemically defined neurons in the hippocampal formation. In this project we concentrated on two topics, quantitative analysis of GABAergic neurons in the hippocampus in the rat and mouse, and characterization of some types of neurons containing calcium binding proteins such as parvalbumin and calretinin. The numbers of chemically defined neurons in the rat and mouse hippocampii have been estimated by a stereological method, optical disector, which revealed th … More e GABAergic neuronal organization of each region and layer in the hippocampus. The areal and laminar distribution of each group of cells shows prominent differences, indicating that the cellular composition was different among regions and from layer to layer. In addition to quantitative disector analyses, we also notice followings ;1) There are astonishing species differences in the calretinin immunoreactivity of the principal neurons of the dentate hilus, mossy cells, which were calretinin positive at the ventral level in the mouse, hamster, gerbil and laboratory shrew whereas they were negative in rat and guinea pig. However, at the dorsal level mossy cell were calretinin negative even in the mouse, hamster, gerbil and laboratory shrew, showing prominent dorsoventral difference in their chemical properties.(2) Parvalbumin containing GABAergic neurons were revealed to make numerous chemical synapses and gap junctions with one another, indicating dual interactions among them and their presumed critical roles in the hippocampal gamma synchronized oscillation. Less

海马形成的神经元组织传统上是基于通过经典形态学方法（例如高尔基体浸渍）定义的神经元类型。但是，免疫细胞化学和其他揭示神经元元素的化学性质的方法的最新进展使得定义神经元的化学亚群，这不一定与经典的形态学定义的神经元类型相对应。在目前的项目中，我们是海马形成中免疫细胞化学定义神经元的旨在合并的光和电子显微镜分析。在这个项目中，我们集中在两个主题上，对大鼠和小鼠海马中的GABA能神经元进行定量分析，以及对某些类型的含有钙结合蛋白的神经元（如白蛋白和calletin）的表征。通过一种立体方法，光学的二元格估计了大鼠和小鼠海马中化学定义的神经元的数量，该方法揭示了每个区域的更多e GABA能神经元组织和海马中的层。每组细胞的面积和层流分布都显示出明显的差异，表明细胞组成在区域之间以及层之间都不同。除了定量不和谐分析外，我们还注意到以下； 1）齿状hilus的主要神经元的calletin免疫反应性差异令人不安，苔藓细胞，苔藓细胞，在小鼠，仓鼠，gerbil和Laboration Shrew and and ane eyea and yealea pighea中，它们在腹侧呈阳性。 However, at the dorsal level mossy cell were calletin negative even in the mouse, hamster, gerbil and laboratory shrew, showing prominent dorsoventral difference in their chemical properties.(2) Parvalbumin containing GABAergic neurons were revealed to make numerous chemical synapses and gap junctions with one another, indicating dual interactions among them and their presumed critical roles in the hippocampal gamma同步振荡。较少的