Cellular organization in the hippocampal formation

海马结构中的细胞组织

基本信息

批准号：
09480213
负责人：
KOSAKA Toshio
金额：
$ 8.51万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

项目摘要

Neuronal organization of the hippocampal formation is traditionally based on the neuronal types defined by classical morphological method such as Golgi impregnation. However recent progress in immunocytochemistry and other methods revealing chemical nature of neuronal elements makes it possible to define chemical subpopulations of neurons, which are not necessarily corresponding to classical morphologically defined neuronal types. In the present project we are intending combined light and electron microscopic analyses of immunocytochemically defined neurons in the hippocampal formation. In this project we concentrated on two topics, quantitative analysis of GABAergic neurons in the hippocampus in the rat and mouse, and characterization of some types of neurons containing calcium binding proteins such as parvalbumin and calretinin. The numbers of chemically defined neurons in the rat and mouse hippocampii have been estimated by a stereological method, optical disector, which revealed th … More e GABAergic neuronal organization of each region and layer in the hippocampus. The areal and laminar distribution of each group of cells shows prominent differences, indicating that the cellular composition was different among regions and from layer to layer. In addition to quantitative disector analyses, we also notice followings ;1) There are astonishing species differences in the calretinin immunoreactivity of the principal neurons of the dentate hilus, mossy cells, which were calretinin positive at the ventral level in the mouse, hamster, gerbil and laboratory shrew whereas they were negative in rat and guinea pig. However, at the dorsal level mossy cell were calretinin negative even in the mouse, hamster, gerbil and laboratory shrew, showing prominent dorsoventral difference in their chemical properties.(2) Parvalbumin containing GABAergic neurons were revealed to make numerous chemical synapses and gap junctions with one another, indicating dual interactions among them and their presumed critical roles in the hippocampal gamma synchronized oscillation. Less

海马结构的神经元组织传统上基于经典形态学方法（例如高尔基浸渍）定义的神经元类型，然而免疫细胞化学和其他揭示神经元元素化学性质的方法的最新进展使得定义神经元的化学亚群成为可能。必然对应于经典形态学定义的神经元类型，在本项目中，我们打算对海马结构中免疫细胞化学定义的神经元进行组合光和电子显微镜分析。我们集中研究了两个主题，即大鼠和小鼠海马中 GABA 能神经元的定量分析，以及含有钙结合蛋白（如小白蛋白和钙结合蛋白）的某些类型神经元的表征。大鼠和小鼠海马中化学定义的神经元的数量。通过体视学方法、光学解剖器进行估计，揭示了海马体每个区域和层的 GABA 神经组织每组细胞的面积和层状分布。显示出显着的差异，表明细胞组成在区域之间和层与层之间存在差异除了定量解剖分析之外，我们还注意到以下几点；1）齿状门主要神经元的钙结合蛋白免疫反应性存在惊人的物种差异。，苔藓细胞，在小鼠、仓鼠、沙鼠和实验室鼩鼱的腹侧水平钙结合蛋白呈阳性，而在大鼠和豚鼠的背部水平呈阴性。即使在小鼠、仓鼠、沙鼠和实验室鼩鼱中，钙视网膜素也呈阴性，这表明它们的化学性质存在显着的背腹差异。(2) 含有 GABA 能神经元的小白蛋白被发现可以相互形成大量化学突触和间隙连接，表明它们之间存在双重相互作用以及它们在海马伽玛同步振荡中的假定关键作用。