Structural analyses of the cellular and synaptic organization in the cortical regions in mice.

小鼠皮质区域细胞和突触组织的结构分析。

基本信息

批准号：
17300112
负责人：
KOSAKA Toshio
金额：
$ 10.03万
依托单位：
Kyushu University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17300112/
关键词：
sensory system limbic system immunocytochemistry confocal laser scanning light microscopy interneuron Proiection neuron association neuron neural circuit 嗅覚系定量解析マウスラット化学シナプスギャップ結合 connexin astrocyte GABA 局所回路網

项目摘要

In this study we analyzed the structural organization of two cortical regions, main olfactory bulb(MOB)and hippocampus in the C57BL/6J strain mice. In the hippocampus we performed quantitative analyses of chemically defined GABAergic neurons by means of a modern stereological method, optical disector using the con focal laser scanning light microscopy, and revealed their regional and laminar composition. Furthermore we performed the quantitative analysis of microglia and revealed their spatial distribution in the hippocampus. In the MOB we performed the immunohistochemical analyses at the light and electron microscopic levels and the tracer experiments, and revealed the followings. 1) We confirmed the compartmental organization of the glomerulus and the presence of type 1 and 2 periglomerular cells. However, the chemical properties of type 1 and 2 periglomerular cells in mice were different from those in rats; that is, both all of tyrosine hydroxylase-positive type 1 periglomerular cel … More ls and the vast majority of type 2 periglomerular cells(all calbindin- and 65% of calretinin-positive periglomerular cells)were GAD/GABA-positive. 2)The nitric oxide synthase(NOS)-containing neurons were heterogeneous, including some subpopulations of periglomerular cells, granule cells, interneurons in the external plexiform layer(EPL), superficial and deep short-axon cells and stellate cells. Importantly some NOS-positive large short-axon cells were projection neurons. In addition, some middle and external tufted cells were also NOS-positive, some of which constituted the intrabulbar association system and the projection system to the olfactory tubercle and the areas covering the dorsal endopiriform nucleus, claustrum and insular cortex. 3)By means of the retrograde tracer experiments some particular types, that is, the larger group but not the smaller group, of tyrosine hydroxylase-positive GABAergic juxtaglomerular neurons were revealed to be the main source of the long intraglomerular connections in the mouse MOB. Less

在这项研究中，我们分析了 C57BL/6J 品系小鼠的两个皮质区域、主嗅球 (MOB) 和海马体的结构组织。在海马体中，我们通过现代体视学方法、光学方法对化学定义的 GABA 能神经元进行了定量分析。此外，我们还对小胶质细胞进行了定量分析，并揭示了它们在海马体中的空间分布。 MOB，我们在光和电子显微镜水平上进行了免疫组织化学分析以及示踪实验，并揭示了以下内容：1）我们确认了肾小球的区室组织以及 1 型和 2 型肾小球周围细胞的化学特性。小鼠的 1 型和 2 型肾小球周围细胞与大鼠的不同；即，均为酪氨酸羟化酶阳性的 1 型肾小球周围细胞……更多ls 和绝大多数 2 型肾小球周围细胞（所有钙结合蛋白和 65% 的钙结合蛋白阳性肾小球周围细胞）均为 GAD/GABA 阳性 2) 含有一氧化氮合酶 (NOS) 的神经元是异质的，包括一些亚群。球周细胞、颗粒细胞、外丛状层（EPL）中间神经元、浅层和深层短轴突重要的是，一些NOS阳性的大短轴突细胞是投射神经元。此外，一些中部和外部的凝结细胞也是NOS阳性的，其中一些构成了球内关联系统和嗅结节的投射系统。以及覆盖背侧内核状核、屏状核和岛叶皮质的区域 3）通过逆行示踪实验，确定了一些特定类型，即较大的组，但不是最大的组。研究表明，较小的一组酪氨酸羟化酶阳性 GABA 能肾小球旁神经元是小鼠 MOB 中长肾小球连接的主要来源。