GABAergic neurons in the central nervous system

中枢神经系统中的 GABA 能神经元

基本信息

批准号：
04404022
负责人：
KOSAKA Toshio
金额：
$ 20.48万
依托单位：
KYUSHU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (A)
财政年份：
1992
资助国家：
日本
起止时间：
1992 至 1994
项目状态：
已结题

项目摘要

Neuronal organization of the central nervous system is traditionally based on the neuronal types defined by classical morphological methods such as Golgi impregnation. However recent progress in immunocytochemistry and other methods revealing chemical nature of neuronal elements makes it possible to define chemical subpopulations of neurons, which are not necessarily corresponding to classical morphologically defined neuronal types. Thus a new classification of neuronal types should be established taking chemical as well as structural characteristics of neurons into consideration. In the present project we are intending combined light and electron microscopic analyzes of immunocytochemically defined interneurons in the cerebral cortex ; that is, olfactory bulb (paleocortex), hippocampus (archicortex) and somatosensory cortex (neocortex). In this project we concentrated on two topics, quantitative analysis of GABAergic and PV neurons in the neocortex and hippocampus and characterization … More of PV neurons in the olfactory bulb. The numbers of neurons in the rat somatosensory cortex and hippocampus have been estimated by a stereological method, disector. Chemical properties of those disector-counted cells were further analyzed by postembedding immunocytochemical methods on adjacent semithin sections. In the rat somatosensory cortex the densities of neurons were 54.9*10^3/mm^3. Of all neurons 25% and 14% were GABA-LIR and PV-IR,respectively ; all PV-IR neurons are GABA-LIR and thus about 54% of GABA-LIR neurons are PV positive. The laminar distribution of each group of cells shows prominent differences, indicating that the cellular composition was different from layr to layr. In the CA1 region the densities of neurons were 35.2*10^3/mm^3. Of all neurons about 7% were considered as GABAergic neurons in the CA1 region. Thus our observations indicated that the proportion of GABAergic neurons is different between hippocampal CA1 region and somatosensory cortex. In the olfactory bulb PV neurons were localized in the external plexiform layr. They were heterogeneous in structural features and classified into 5 types. However they made reciprocal synapses with mitral/tufted cells, which had been believed to be formed only between granule cells and mitral/tufted cells. Less

传统上，中枢神经系统的神经元组织是基于通过古尔基浸渍等经典形态学方法定义的神经元类型。但是，免疫细胞化学和其他揭示神经元元素的化学性质的方法的最新进展使得定义神经元的化学亚群，这不一定与经典的形态学定义的神经元类型相对应。应考虑化学物质以及神经元的结构特征的新分类神经元类型。在本项目中，我们是针对脑皮质中免疫细胞化学定义的中间神经元的光线和电子显微镜分析的组合。也就是说，嗅球（古皮质），海马（ArchicCortex）和体感皮质（Neocortex）。在这个项目中，我们集中于两个主题，即新皮层和海马中的GABA能和PV神经元的定量分析以及嗅球中更多的PV神经元。大鼠体感皮质和海马中的神经元数量已通过立体方法估算，即疾病。通过在相邻的semithin切片上的免疫细胞化学方法进行后，进一步分析了这些乳房计数细胞的化学特性。在大鼠体感皮层中，神经元的密度为54.9*10^3/mm^3。在所有神经元中，分别为25％和14％，分别为GABA-LIR和PV-IR；所有PV-IR神经元都是GABA-LIR，因此约有54％的GABA LIR神经元为PV阳性。每组细胞的层分布显示出明显的差异，表明细胞组成因LAYR到LAYR的不同而不同。在CA1区域，神经元的密度为35.2*10^3/mm^3。在所有约7％的神经元中，在CA1区域被认为是GABA能神经元。我们的观察结果表明，海马CA1区域和体感皮质之间的GABA能神经元的比例不同。在嗅球中，PV神经元位于外部丛状外行中。它们在结构特征中是异质的，分为5种类型。但是，他们与二尖瓣/簇细胞制作了相互的突触，据信仅在颗粒细胞和二尖瓣/簇细胞之间形成。较少的