A simple system for developing the microsatellite markers using RSE-PCR and pyrosequencing

使用 RSE-PCR 和焦磷酸测序开发微卫星标记的简单系统

• 批准号: 24658138
• 负责人: SHIRAISHI Susumu
• 金额: $ 2.5万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24658138/
• 关键词: DNAマーカー; マイクロサテライト; 4塩基繰返し; 森林科学; 育種学; 分子生態学; ４塩基繰返し

DNA molecular markers were widely utilized as powerful tool in breeding, genetics, and ecology. Among these markers, microsatellite marker is highly variable, and possesses a large quantity of genetic information. Constructing a new system, a next generation sequencer (pyrosequencing) was introduced to gain the huge sequences of microsatellite regions in genomes. The new system showed the possibility for a simple and inexpensive microsatellite development.

DNA分子标记作为强大的工具被广泛应用于育种、遗传学和生态学领域。在这些标记中，微卫星标记变异性强，拥有大量的遗传信息。构建了一个新系统，引入了下一代测序仪（焦磷酸测序）来获得基因组中微卫星区域的巨大序列。新系统展示了简单且廉价的微型卫星开发的可能性。

项目成果

Development of tetranucleotide microsatellite markers in Pinus kesiya Royle ex Gordon

凯西亚松四核苷酸微卫星标记的开发

• DOI: 10.1007/s12686-012-9814-3
• 发表时间: 2012
• 期刊: Conservation Genetics Resources
• 影响因子: 1.1
• 作者: D. V. Thao;M. Yamashita;A. Watanabe;S. Shiraishi
• 通讯作者: S. Shiraishi

Isolation and characterization of tetranucleotide microsatellite markers for Pinus merkusii

梅库斯松四核苷酸微卫星标记的分离和表征

• 发表时间: 2013
• 期刊: Conservation Genet Resour
• 作者: Thao DV;Widyatmoko AYPBC;Guan L Watanabe A;Gotoh E;Shiraishi S
• 通讯作者: Shiraishi S