Global transcriptome analysis of the pine wilt disease and application to the resistance breeding

松材线虫病全转录组分析及其在抗性育种中的应用

• 批准号: 21380100
• 负责人: SHIRAISHI Susumu
• 金额: $ 11.23万
• 依托单位: Kyushu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21380100/
• 关键词: クロマツ; マツ材線虫病; トランスクリプトーム; 抵抗性育種; 遺伝子発現; 植物; 遺伝子; 病理学; 抵抗性; ODD

The Japanese black pine(Pinus thunbergii) is highly susceptible to pine wilt disease caused by the pine wood nematode(PWN ; Bursaphelenchus xylophilus). To cope with this disease, researchers and tree breeders selected PWN-resistant individuals in a previous breeding program. In an attempt to understand the mechanisms of pathopoiesis and resistance, the mechanisms were clarified using LongSAGE(serial analysis of gene expression) and ODD(ordered differential display) analyses. Especially, by the global transcriptome analysis using SAGE, it is indicated that pathogenesis-related proteins, osmotin, lipoxygenase, chalcone synthase were significantly up-regulated, and eukaryotic translation initiation factor SUI1, translationally controlled tumor protein, and xyloglucan endotransglycosylase were down-regulated by the PWN inoculation in both the resistant and non-resistant pines. On the other hand, catalase, dienelactone hydrolase family protein were expressed at a higher level only in the resistant pine, pathogenesis-related proteins and leucoanthocyanidin dioxygenase were expressed only in the non-resistant pine. These differentially expressed genes are presumed to reflect some of the differences between the resistant and non-resistant pines.

日本黑松（Pinus thunbergii）非常容易受到松树线虫（PWN; bursaphelenchus xylelophilus）引起的松木疾病。为了应对这种疾病，研究人员和育种者在以前的繁殖计划中选择了抗PWN的个体。为了理解病原体和抗性的机制，使用LONGSAGE（基因表达的序列分析）和奇数（有序的差分显示）分析来阐明这些机制。特别是，通过使用SAGE的全球转录组分析，表明与发病机理相关的蛋白质，渗透蛋白，Lipoxygogyase，Chalcone合酶显着上调，真核翻译起始因子SUI1，翻译受控的肿瘤蛋白和木糖酸乳腺癌含量是腹膜内氟糖基酶溶剂溶质化酶下部质丙糖基酶基因溶剂溶液化酶通过耐药和非耐药松树中的PWN接种。另一方面，仅在抗性松树，与发病机理相关的蛋白和白细胞促蛋白二氧酶的抗性松树中，二烯酮水解酶家族蛋白仅在较高的水平上表达，仅在非耐药的松树中表达。假定这些差异表达的基因可以反映抗性和非耐药松果之间的某些差异。

项目成果

マツノザイセンチュウを接種されたクロマツ(Pinus thunbergii)の遺伝子発現プロファイル

接种黑松的黑松基因表达谱

• 发表时间: 2010
• 作者: Fukuzawa;K.;管蘭華・白石進
• 通讯作者: 管蘭華・白石進

SAGE法を用いた網羅的な発現遺伝子解析.マツ材線虫病抵抗性のメカニズム解明に向けて.

采用SAGE方法进行综合基因表达分析，阐明松材线虫抗病机制。

• 发表时间: 2012
• 期刊: 林木の育種
• 作者: 能勢美峰;白石進
• 通讯作者: 白石進

Comparison of the gene expression profiles of resistant and non-resistant Japanese black pine inoculated with pine wood nematode using a modified LongSAGE technique

• DOI: 10.1111/j.1439-0329.2010.00646.x
• 发表时间: 2011-04-01
• 期刊: FOREST PATHOLOGY
• 影响因子: 1.4
• 作者: Nose, M.;Shiraishi, S.
• 通讯作者: Shiraishi, S.