Postdoctoral Fellowship: MPS-Ascend: Elucidation of Peptide Scrambling in Collision Induced Dissociation: Energetic and Mechanistic Analysis via Guided Ion Beam Mass Spectrometry

博士后奖学金：MPS-Ascend：阐明碰撞诱导解离中的肽加扰：通过引导离子束质谱进行能量和机理分析

• 批准号: 2316669
• 负责人: Evan Perez
• 金额: $ 30万
• 依托单位: Perez, Evan Hunt
• 依托单位国家: 美国
• 项目类别: Fellowship Award
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-08-01 至 2026-07-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2316669&HistoricalAwards=false
• 关键词: Postdoctoral; Fellowship; MPS; Ascend; Elucidation

Evan H. Perez is awarded an NSF Mathematical and Physical Sciences Ascending Postdoctoral Research Fellowship (NSF MPS-Ascend) to conduct a program of research and activities related to broaden participation by groups underrepresented in STEM. This fellowship to Dr. Perez supports the research project entitled “MPS-Ascend: Elucidation of Peptide Scrambling in Collision Induced Dissociation: Energetic and Mechanistic Analysis via Guided Ion Beam Mass Spectrometry” under the mentorship of a sponsoring scientist. The host institution for the fellowship is the University of Utah, and the sponsoring scientist is Dr. Peter B. Armentrout.This proposal seeks to deepen the understanding of peptide fragmentation and rearrangement mechanisms by using Guided Ion Beam Mass Spectrometry to observe energy-resolved collision events and model these energies using electronic structure calculations. The proposal will use polyglycine polymer ions to perform competitive threshold analysis and electronic structure calculations to elucidate the key chemical pathways for the observed fragmentation events. The proposed research will investigate the role of the peptide length from tetraglycine up to hexaglycine in the formation of macrocyclic structures which precedes the intramolecular fragmentation events known as “scrambling.” The work will implement traveling wave ion mobility SLIM (Structures for Lossless Ion Manipulation) experiments to allow for conformation specific fragmentation analysis as well as conformer analysis of the fragment ions. The proposed studies will enable comparison of fragmentation methods (hard single collision versus soft collisional heating) and correlation of conformation with mechanism. Successful implementation of this work may lead to improved understanding of peptide sequencing by mass spectrometry which could have significant impacts on determining protein sequences and advancing the proteomics field.The PI plans to mentor undergraduate and graduate students from underrepresented minority groups in research projects involving training in mass spectrometric techniques and learning quantum chemical calculations. The PI intends to participate with the STEM Action Center of the University of Utah in potential recruiting activities and mentoring of students from underrepresented groups.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

Evan H. Perez 获得 NSF 数学和物理科学提升博士后研究奖学金 (NSF MPS-Ascend)，用于开展一项与扩大 STEM 领域代表性不足的群体参与相关的研究和活动计划。该奖学金支持 Perez 博士的研究项目。标题为“MPS-Ascend：阐明碰撞诱导解离中的肽扰乱：通过引导离子束质量进行能量和机械分析”该奖学金的主办机构是犹他大学，赞助科学家是 Peter B. Armentrout 博士。该提案旨在通过使用引导加深对肽断裂和重排机制的理解。离子束质谱法观察能量分辨碰撞事件，并使用电子结构计算对这些能量进行建模，该提案将使用聚甘氨酸聚合物离子进行竞争阈值分析和电子结构计算，以阐明关键。拟议的研究将研究从四甘氨酸到六甘氨酸的肽长度在大环结构形成中的作用，该过程发生在被称为“加扰”的分子内断裂事件之前。 SLIM（无损离子操作结构）实验允许进行构象特异性碎裂分析以及碎片离子的构象异构体分析。拟议的研究将能够对碎裂方法进行比较。 （硬单次碰撞与软碰撞加热）以及构象与机制的相关性这项工作的成功实施可能会提高对质谱肽测序的理解，这可能对确定蛋白质序列和推进蛋白质组学领域产生重大影响。PI 计划。 PI 打算与犹他大学 STEM 行动中心一起参与潜在的学生招募活动和指导。来自代表性不足的群体。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。