MRI: Track 1: Acquisition of a MALDI-TOF-TOF Mass Spectrometer with TOF/TOF Capabilities

MRI：轨道 1：获取具有 TOF/TOF 功能的 MALDI-TOF-TOF 质谱仪

• 批准号: 2319960
• 负责人: Scott Grayson
• 金额: $ 49万
• 依托单位: Tulane University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-01 至 2026-08-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2319960&HistoricalAwards=false
• 关键词: MRI; Track; Acquisition; MALDI; TOF

This award is jointly funded by the Major Research Instrumentation Program, the Chemistry Research Instrumentation Program, the Macromolecular, Supramolecular, and Nanochemistry (MSN) program in Chemistry, and the Established Program to Stimulate Competitive Research (EPSCoR). This award will support several universities within the New Orleans area, including Tulane, Dillard, and Loyola. The acquisition of a matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI-ToF MS) will be used by researchers in the departments of chemistry, chemical engineering, and physics. MALDI-ToF MS is one of the main tools for the characterization of large molecules (having a molecular weight of greater than 500) including peptides, proteins, nucleic acids, carbohydrates, and polymers. The new instrument will have high resolution and tandem MS capabilities. This instrument will strengthen the mass spectrometry community within New Orleans and ensure that undergraduate and graduate students at the participating institutions will have access to state-of-the-art mass spectrometry instrumentation. In addition, high school students, 80% of whom are African American, will learn about polymers and how MALDI-ToF MS can determine molecular weights and repeating units.The acquisition of a MALDI-ToF MS will enhance existing MS capabilities and enable new research not currently possible at Tulane, Dillard and Loyola. These new capabilities will elucidate the interaction of structure and molecular weight for a variety of large molecules. For example, the degradation of hexadecapeptide found in Alzheimer’s patients can be investigated by the arrangement of the labile amino acids. Topological transitions of DNA will also be investigated using gyrase variants in bacteria. A number of these gyrase variants have higher (proline) or lower (glycine) flexibility, and it will be useful to determine the high molecular weight degradation products of these compounds. The polymer structure of bis(hydroxymethyl)propanoic acid (purely linear, purely dendritic, or randomly hyperbranched) will also be investigated. The new instrumentation will also be used to characterize the end groups of poly(ethylene glycol) polymers. There will be many mass spectrometry analyses that the new instrumentation can do quite easily, including DNA, cavitands, inorganic complexes, etc. that will be hard or impossible without this instrument.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该奖项由重大研究仪器计划、化学研究仪器计划、化学大分子、超分子和纳米化学（MSN）计划以及刺激竞争性研究既定计划（EPSCoR）联合资助。该奖项将支持多所大学。新奥尔良地区，包括杜兰大学、迪拉德大学和洛约拉大学获得基质辅助激光解吸/电离飞行时间质量。光谱仪（MALDI-ToF MS）将被化学、化学工程和物理系的研究人员使用，是表征大分子（分子量大于500）的主要工具之一。该新仪器将具有高分辨率和串联质谱功能，将加强新奥尔良的质谱界，并确保参与机构的本科生和研究生能够参与其中。此外，高中生（其中 80% 是非裔美国人）将了解聚合物以及 MALDI-ToF MS 如何确定分子量和重复单元。 MALDI-ToF MS 将增强现有的 MS 功能，并使杜兰大学、迪拉德大学和洛约拉大学目前无法进行的新研究成为可能。这些新功能将阐明各种大分子的结构和分子量的相互作用。阿尔茨海默氏症患者中发现的十六肽的降解可以通过不稳定氨基酸的排列来研究，也可以使用细菌中的旋转酶变体来研究这些旋转酶变体中的一些具有更高的（脯氨酸）或更低的（甘氨酸）。灵活性，并且有助于确定这些化合物的高分子量降解产物双（羟甲基）丙酸的聚合物结构（纯线性、纯树枝状或无规）。新仪器也将用于表征聚乙二醇聚合物的端基。新仪器可以很容易地进行许多质谱分析，包括 DNA、空配体、无机复合物。等等，如果没有这个工具，这将是困难或不可能的。该奖项反映了 NSF 的法定使命，并且通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。