CAREER: Testing the effects of predator-derived feces on host symbiont acquisition and health

职业：测试捕食者粪便对宿主共生体获取和健康的影响

• 批准号: 2145472
• 负责人: Adrienne Simoes Correa
• 金额: $ 98.22万
• 依托单位: William Marsh Rice University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-04-01 至 2027-03-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2145472&HistoricalAwards=false
• 关键词: CAREER; Testing; effects; predator; derived

Climate change and local-scale anthropogenic stressors are degrading coral reefs across the globe. When conditions become too stressful on reefs, corals can lose beneficial microbial symbionts (e.g., dinoflagellates in the family Symbiodiniaceae) that live in their tissues via a process called “bleaching”. Although Symbiodiniaceae play key roles in the health of coral colonies, we know little about the processes that make symbionts available in the environment to prospective host corals. This research test the extent to which coral-eating fish feces, which contain live Symbiodiniaceae, facilitate symbiont acquisition by corals in their early life stages. It will generate seminal knowledge on how corallivore feces impact coral symbioses and health, and will assess the ecological importance of corallivorous fishes as drivers of coral symbiont assemblages. This research also test the extent to which corallivore feces are a source of food and nutrients that impact coral health; this has particular relevance to the survival and recovery of bleached adult corals. This research can ultimately inform intervention strategies to support reef resilience and mitigate reef degradation. Results from this project will be communicated widely in scientific arenas, in undergraduate education programs, and to the public via multimedia content and outreach. The Houston Independent School District (HISD, Houston, TX) is the nation’s 7th largest public school system. This work will enrich environmental science curricula for underrepresented minority students at under-resourced HISD high schools. This work will also support economically disadvantaged and first-generation undergraduate students in pursuing STEM majors and careers through multi-year research experiences.Symbioses between foundation species (e.g., corals, sponges, trees) and microbiota (e.g., microeukaryotes, bacteria) underpin the biodiversity, productivity, and stability of ecosystems. Consumers, such as predators and herbivores, shape communities of these foundation species through trophic interactions. For instance, grazers contribute to the maintenance of coral dominance on reefs via consumption of macroalgal competitors. However, the indirect effects of other consumers on foundation species are rarely examined. Few studies have tested how consumers affect microbiota assembly in corals, even though coral symbionts (e.g., dinoflagellates in the family Symbiodiniaceae) play key roles in reef function and persistence. Corallivorous (coral-eating) fishes were recently demonstrated to egest large quantities of live Symbiodiniaceae cells as they swim across reefs. This research is testing the hypothesis that corallivore feces promote coral dominance on reefs by supporting coral acquisition of key symbionts and nutrients. The following research objectives will be accomplished: (1) to quantify the contribution of corallivorous fish feces to coral symbiont acquisition; and (2) to test the extent to which corallivorous fish feces influence coral health and recovery from thermal stress. Reefs are being degraded globally due to climate-change induced bleaching and associated mortality. This project is teasing apart the extent to which nutrients and/or live symbionts associated with corallivore feces contribute to the resilience of bleached corals under ambient and heat stress conditions. The research is tightly integrated with two education objectives: (1) to organize a Research Experience for Teachers (RET) program in which rigorous learning modules that high school teachers can incorporate into their Environmental Systems course offerings are developed and tested; and (2) to provide undergraduate students with a multi-year research experience through a partnership with the Rice Emerging Scholars Program (RESP).This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

气候变化和局部人为压力正在导致全球珊瑚礁退化。当珊瑚礁承受的压力过大时，珊瑚可能会通过一种称为“白化”的过程失去生活在其组织中的有益微生物共生体（例如共生藻科的甲藻）。尽管共生体科在珊瑚群落的健康中发挥着关键作用，但我们对使共生体在环境中为潜在宿主提供的过程知之甚少。这项研究测试了食珊瑚鱼的粪便（含有活的共生藻）在多大程度上促进珊瑚在生命早期阶段获得共生体，它将产生关于食珊瑚动物粪便如何影响珊瑚共生和健康的重要知识，并将评估珊瑚的共生体。食珊瑚鱼作为珊瑚共生体组合驱动因素的生态重要性这项研究还测试了食珊瑚鱼粪便作为影响珊瑚的食物和营养来源的程度。该研究最终可以为支持珊瑚礁恢复力和减轻珊瑚礁退化的干预策略提供信息，该项目的结果将在科学领域、本科教育项目和中广泛传播。休斯顿独立学区（HISD，德克萨斯州休斯顿）是美国第七大公立学校系统，这项工作将丰富资源匮乏的 HISD 高中中代表性不足的少数族裔学生的环境科学课程。这项工作还将通过多年的研究经验，支持经济上处于不利地位的第一代本科生攻读 STEM 专业和职业。基础物种（例如珊瑚、海绵、树木）和微生物群（例如微真核生物、细菌）之间的共生关系。食肉动物和食草动物等消费者通过营养相互作用塑造这些基础物种的群落。然而，很少有研究测试消费者如何影响珊瑚中的微生物群，尽管珊瑚共生体（例如，甲藻）。共生科（Symbiodiniaceae）在珊瑚礁功能和持久性方面发挥着关键作用，食珊瑚（食珊瑚）鱼最近在游泳时会摄取大量活的共生科细胞。这项研究正在检验这样的假设：食珊瑚鱼的粪便通过支持珊瑚获取关键共生体和营养物质来促进珊瑚在珊瑚礁上的优势，将实现以下研究目标：（1）量化食珊瑚鱼粪便对珊瑚共生体获取的贡献。 (2) 测试食珊瑚鱼粪便对珊瑚健康和热应激恢复的影响程度。由于气候变化引起的白化和相关死亡，全球珊瑚礁正在退化。该研究与两个教育目标紧密结合：（1）为教师组织一次研究体验。 (RET) 计划，其中高中教师可以将严格的学习模块纳入其环境系统课程中进行开发和测试；(2) 通过与莱斯新兴学者计划的合作，为本科生提供多年的研究经验； (RESP)。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Viruses of a key coral symbiont exhibit temperature-driven productivity across a reefscape

关键珊瑚共生体的病毒在整个珊瑚礁景观中表现出温度驱动的生产力

• DOI: 10.1038/s43705-023-00227-7
• 发表时间: 2023-04-03
• 期刊: ISME Communications
• 作者: Howe-Kerr, Lauren I.;Grupstra, Carsten G. B.;Rabbitt, Kristen M.;Conetta, Dennis;Coy, Samantha R.;Klinges, J. Grace;Maher, Rebecca L.;McConnell, Kaitlin M.;Meiling, Sonora S.;Messyasz, Adriana;Schmeltzer, Emily R.;Seabrook, Sarah;Sims, Jordan A.;Veglia, Alex J.;Thurber, Andrew R.;Thurber, Rebecca L. Vega;Correa, Adrienne M. S.
• 通讯作者: Correa, Adrienne M. S.

Consumer feces impact coral health in guild-specific ways

消费者粪便以特定方式影响珊瑚健康

• DOI: 10.3389/fmars.2023.1110346
• 发表时间: 2023-04
• 期刊: Frontiers in Marine Science
• 影响因子: 3.7
• 作者: Grupstra, Carsten G.;Howe;van der Meulen, Jesse A.;Veglia, Alex J.;Coy, Samantha R.;Correa, Adrienne M.
• 通讯作者: Correa, Adrienne M.

Endogenous viral elements reveal associations between a non-retroviral RNA virus and symbiotic dinoflagellate genomes

内源病毒元件揭示非逆转录病毒RNA病毒与共生甲藻基因组之间的关联

• DOI: 10.1038/s42003-023-04917-9
• 发表时间: 2023-06-01
• 期刊: Communications Biology
• 影响因子: 5.9
• 作者: Veglia, Alex J.;Bistolas, Kalia S. I.;Voolstra, Christian R.;Hume, Benjamin C. C.;Ruscheweyh, Hans-Joachim;Planes, Serge;Allemand, Denis;Boissin, Emilie;Wincker, Patrick;Poulain, Julie;Moulin, Clementine;Bourdin, Guillaume;Iwankow, Guillaume;Romac, Sarah;Agostini, Sylvain;Banaigs, Bernard;Boss, Emmanuel;Bowler, Chris;de Vargas, Colomban;Douville, Eric;Flores, Michel;Forcioli, Didier;Furla, Paola;Galand, Pierre E.;Gilson, Eric;Lombard, Fabien;Pesant, Stephane;Reynaud, Stephanie;Sunagawa, Shinichi;Thomas, Olivier P.;Trouble, Romain;Zoccola, Didier;Correa, Adrienne M. S.;Thurber, Rebecca L. Vega L.
• 通讯作者: Thurber, Rebecca L. Vega L.

Thank you for biting: dispersal of beneficial microbiota through 'antagonistic' interactions

感谢您的叮咬：通过“拮抗”相互作用传播有益微生物群

• DOI: 10.1016/j.tim.2022.03.006
• 发表时间: 2022-04
• 期刊: Trends in Microbiology
• 影响因子: 15.9
• 作者: Grupstra, C.G.B.;Lemoine, N.P.;Cook, C.;Correa, A.M.S.
• 通讯作者: Correa, A.M.S.

Filamentous virus-like particles are present in coral dinoflagellates across genera and ocean basins

丝状病毒样颗粒存在于跨属和洋盆的珊瑚甲藻中

• DOI: 10.1038/s41396-023-01526-6
• 发表时间: 2023-12
• 期刊: The ISME Journal
• 影响因子: 11
• 作者: Howe-Kerr, Lauren I.;Knochel, Anna M.;Meyer, Matthew D.;Sims, Jordan A.;Karrick, Carly E.;Grupstra, Carsten G. B.;Veglia, Alex J.;Thurber, Andrew R.;Thurber, Rebecca L. Vega;Correa, Adrienne M. S.
• 通讯作者: Correa, Adrienne M. S.