MRI: Acquisition of Fluorescence Activated Cell Sorter (FACS) for Multidisciplinary Research and Education at Fordham University

MRI：福特汉姆大学采购荧光激活细胞分选仪 (FACS) 用于多学科研究和教育

• 批准号: 2117625
• 负责人: Ipsita Banerjee
• 金额: $ 15.29万
• 依托单位: Fordham University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-10-01 至 2024-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2117625&HistoricalAwards=false
• 关键词: MRI; Acquisition; Fluorescence; Activated; Cell

An award is made to Fordham University for the acquisition of a BD FACSMelody (BYG 6-Color Plate 4 way) fluorescence activated cell sorter (FACS) for a multiuser facility in the Chemistry Department at Fordham University. Acquisition of a state of the art instrument such as FACS will improve the research infrastructure and advance faculty research and training programs of students and postdocs across two campuses. It will enhance collaborative research opportunities between the Departments of Biological Sciences, Chemistry, Psychology and Natural Sciences as well as the broader community. It will also expose a growing cohort of undergraduate science majors to a powerful experimental tool in biochemistry. Students and faculty from minority institutions, including local community colleges and high schools, conducting research at Fordham will greatly benefit from learning such advanced techniques. In addition, it will help provide meaningful research and hands-on experience to undergraduate students through investigative laboratory experiments as part of the recently established Biochemistry minor and aid in expanding the Biochemistry program. It will enhance student’s ability to understand intricate cellular mechanisms, and provide them with the skills and scientific acumen to join the STEM workforce and participate in the society as scientifically literate individuals. Additionally, faculty plan on outreach activities such as creating podcasts for local senior citizen organizations so that the community’s vulnerable population can become more aware about cellular and physiological processes involved in diseases. Furthermore, workshops for local high school students will be held. These activities will promote recruitment, retention, and professional development of a diverse STEM workforce. The instrument will facilitate interdisciplinary research in key areas including tissue engineering, single-molecule biophysics, protein engineering, environmental science, neuropsychology and neuroscience. It will provide a cutting-edge multi-user instrument with high sensitivity for quantitative and qualitative investigation of a variety of cell populations in a high-throughput manner. FACS is a crucial tool for the separation of desired cell populations in high purity and analysis of single cells based on cell markers, size, and interactions. FACS is also the only purification technique to isolate and sort cells based on intracellular protein expression in a rapid and highly efficient manner. Thus, the FACS instrument will be employed in a variety of projects that include examining stem cell differentiation pathways at cell-biomaterial interfaces, investigating the molecular mechanisms of high-fidelity and error-prone DNA replication in bacteria, studying the environmental impacts of novel ionic liquids, examining cellular uptake of engineered peptides, studying the behavior of neuronal populations in transgenic zebrafish, examining the effects of stress hormones on leukocytes, as well as elucidating the mechanism of viral entry and trafficking to reduce HPV related infections. The research afforded by the instrument will allow Fordham faculty to make substantive contributions to a variety of important developing areas of biotechnology. As an institution in one of the most diverse cities in the country, the FACS instrument will provide an exceptional opportunity to impart high-quality research and teaching to a diverse student population at Fordham.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

福特汉姆大学因为福特汉姆大学化学系的多用户设施购买 BD FACSMelody（BYG 6 色板 4 路）荧光激活细胞分选仪 (FACS) 而获得了一项最先进的仪器。 FACS 等项目将改善两个校区的研究基础设施并推进学生和博士后的教师研究和培训项目，这将增加生物科学、化学、心理学系之间的合作研究机会。它还将使越来越多的理科专业本科生接触到强大的生物化学实验工具，包括当地社区学院和高中，在福特汉姆进行研究将大大提高。此外，作为最近设立的生物化学辅修课程的一部分，它将有助于通过研究性实验室实验为本科生提供有意义的研究和实践经验，并有助于扩展生物化学课程。了解复杂的细胞机制，并为他们提供加入 STEM 劳动力并作为具有科学素养的个人参与社会的技能和科学敏锐度。此外，教师还计划开展外展活动，例如为当地老年人组织创建播客，以便社区的弱势群体能够成为其中的一员。此外，将为当地高中生举办研讨会，促进多元化 STEM 劳动力的招募、保留和专业发展，包括促进关键领域的跨学科研究。组织工程，单分子它将为生物物理学、蛋白质工程、环境科学、神经心理学和神经科学提供一种高灵敏度的尖端多用户仪器，用于以高通量方式对各种细胞群进行定量和定性研究。基于细胞标记、大小和相互作用对所需细胞群进行高纯度分离和高度单细胞分析也是唯一一种基于细胞内蛋白质表达快速有效地分离和分选细胞的纯化技术。 ，FACS 仪器将应用于各种项目，包括检查细胞-生物材料界面的干细胞分化途径、研究细菌中高保真和易错 DNA 复制的分子机制、研究新型离子液体的环境影响、工程肽的细胞摄取，研究转基因斑马鱼神经元群体的行为，检查应激激素对白细胞的影响，以及阐明病毒进入和运输的机制以减少 HPV 相关感染。该仪器提供的研究将允许。福特汉姆大学的教师将为生物技术的各个重要发展领域做出实质性贡献，作为美国最多元化城市之一的机构，FACS 仪器将为向多元化的学生传授高质量的研究和教学提供绝佳的机会。这反映了 NSF 的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Probing the Interactions of LRP1 Ectodomain-Derived Peptides with Fibrillar Tau Protein and Its Impact on Cellular Internalization

探讨 LRP1 胞外域衍生肽与纤维状 Tau 蛋白的相互作用及其对细胞内化的影响

• DOI: 10.3390/app13020853
• 发表时间: 2023-01
• 期刊: Applied Sciences
• 作者: Boder, E. Josephine;Goncalves, Beatriz G.;Lebedenko, Charlotta G.;Banerjee, Ipsita A.
• 通讯作者: Banerjee, Ipsita A.

A computational and laboratory approach for the investigation of interactions of peptide conjugated natural terpenes with EpHA2 receptor

研究肽缀合天然萜烯与 EpHA2 受体相互作用的计算和实验室方法

• DOI: 10.1007/s00894-023-05596-3
• 发表时间: 2023-06-08
• 期刊: Journal of Molecular Modeling
• 影响因子: 2.2
• 作者: Beatriz G. Goncalves;I. Banerjee
• 通讯作者: I. Banerjee

Gene Expression and DNA Methylation in Human Papillomavirus Positive and Negative Head and Neck Squamous Cell Carcinomas

人乳头瘤病毒阳性和阴性头颈鳞状细胞癌中的基因表达和 DNA 甲基化

• DOI: 10.3390/ijms231810967
• 发表时间: 2022-09-19
• 期刊: International Journal of Molecular Sciences
• 影响因子: 5.6
• 作者: Hinic, Snezana;Rich, April;Anayannis, Nicole, V;Cabarcas-Petroski, Stephanie;Schramm, Laura;Meneses, Patricio, I
• 通讯作者: Meneses, Patricio, I

The Process of Filopodia Induction during HPV Infection

HPV感染过程中丝状伪足的诱导过程

• DOI: 10.3390/v14061150
• 发表时间: 2022-05-26
• 期刊: Viruses
• 作者: Biondo, Alyssa;Meneses, Patricio I.
• 通讯作者: Meneses, Patricio I.

Interactions of Nanoscale Self-Assembled Peptide-Based Assemblies with Glioblastoma Cell Models and Spheroids

• DOI: 10.1021/acsomega.2c08049
• 发表时间: 2023-04-04
• 期刊: ACS Omega
• 影响因子: 4.1
• 作者: Lebedenko, Charlotta G.;Murray, Molly E.;Goncalves, Beatriz G.;Perez, Diego S.;Lambo, Dominic J.;Banerjee, Ipsita A.
• 通讯作者: Banerjee, Ipsita A.