MRI: Track 1 Acquisition of a fluorescence activated cell sorter to advance multidisciplinary biological research and training at the University of Memphis

MRI：轨道 1 采购荧光激活细胞分选仪，以推进孟菲斯大学的多学科生物学研究和培训

• 批准号: 2319905
• 负责人: Jennifer Mandel
• 金额: $ 42.94万
• 依托单位: University of Memphis
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-10-01 至 2026-09-30
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2319905&HistoricalAwards=false
• 关键词: MRI; Track; Acquisition; fluorescence; activated

An award is made to the University of Memphis to support the acquisition of a BD FACSMelody Fluorescence-Activated Cell Sorter (FACS) for a shared, multiuser facility. The FACS will enhance a broad range of multidisciplinary and collaborative research among the University of Memphis and Memphis area research institutions and enrich the training of the next generation of biologists. This instrument will enable researchers to separate cell populations based on specific cell surface markers or cell size and evaluate them for specific cellular functions. The FACS will allow current researchers to address exciting new basic science and help to attract future researchers thus improving the capacity to perform cutting-edge research at the University of Memphis. The acquisition and use of this instrument will strengthen connections with the Memphis Zoo and provide educational opportunities to two Primarily Undergraduate Institutions and Historically Black Colleges and Universities (HBCUs) in the Memphis area. The FACS will improve research infrastructure, enhance faculty research, and expand the training of undergraduates, graduate students, and postdoctoral researchers in the Department of Biological Sciences, the College of Health Sciences, other STEM programs at the university, and the Memphis scientific community. The University of Memphis along with collaborating institutions serves a diverse population of trainees and the acquisition of the FACS will ensure students and postdoctoral researchers from different backgrounds will have the opportunity to learn to use the FACS instrument. The project will also enhance networking and collaborative opportunities through an annual mini-symposium and outreach activities with local institutions, including HBCUs and the Memphis Zoo. The BD FACSMelody Fluorescence-Activated Cell Sorter (FACS) rapidly separates cells into groups based on size and cell-surface markers that give the cell a unique set of characteristics (e.g., the insulin receptor and insulin-dependent glucose transport). This allows researchers to address a wide range of multidisciplinary biological questions including the regulation of gene expression, the evolution of adaptive changes to the environment, and how information is transferred from the outside to the inside of the cell. Projects will focus on 1) the genetic basis for morphological traits in the sunflower family; 2) changes in immune cell populations associated with obesity, aging, and disease; 3) how receptor trafficking regulates cellular responses; 4) the single cell genomic analysis of stem cells; 5) the impact of environmental toxins on skin function; 6) the analysis of gene expression in the sensory neurons of ant antennae; and 7) endangered species conservation by enhancing techniques for assisted reproduction. The work that results from this project will be shared with the scientific community through publications within peer-reviewed articles, presentations at scientific meetings, and sharing to social media.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

孟菲斯大学获得了一项奖励，用于支持为共享的多用户设施购买 BD FACSMelody 荧光激活细胞分选仪 (FACS)。 FACS将加强孟菲斯大学和孟菲斯地区研究机构之间广泛的多学科和合作研究，并丰富下一代生物学家的培训。该仪器将使研究人员能够根据特定的细胞表面标记或细胞大小分离细胞群，并评估它们的特定细胞功能。 FACS 将使当前的研究人员能够解决令人兴奋的新基础科学问题，并帮助吸引未来的研究人员，从而提高孟菲斯大学进行尖端研究的能力。该仪器的购买和使用将加强与孟菲斯动物园的联系，并为孟菲斯地区的两所主要本科院校和历史黑人学院和大学 (HBCU) 提供教育机会。 FACS 将改善研究基础设施，加强教师研究，并扩大对生物科学系、健康科学学院、大学其他 STEM 项目以及孟菲斯科学界的本科生、研究生和博士后研究人员的培训。孟菲斯大学与合作机构为不同的学员群体提供服务，收购 FACS 将确保来自不同背景的学生和博士后研究人员有机会学习使用 FACS 仪器。该项目还将通过年度小型研讨会以及与当地机构（包括 HBCU 和孟菲斯动物园）的外展活动来加强网络和合作机会。 BD FACSMelody 荧光激活细胞分选仪 (FACS) 根据大小和细胞表面标记快速将细胞分组，这些标记赋予细胞一组独特的特征（例如胰岛素受体和胰岛素依赖性葡萄糖转运）。这使得研究人员能够解决广泛的多学科生物学问题，包括基因表达的调节、环境适应性变化的进化，以及信息如何从细胞外部转移到细胞内部。项目将重点关注 1) 向日葵家族形态性状的遗传基础； 2）与肥胖、衰老和疾病相关的免疫细胞群的变化； 3）受体运输如何调节细胞反应； 4）干细胞的单细胞基因组分析； 5）环境毒素对皮肤功能的影响； 6）蚂蚁触角感觉神经元基因表达分析； 7) 通过加强辅助生殖技术来保护濒危物种。 该项目的成果将通过同行评审文章中的出版物、科学会议上的演示以及社交媒体分享的方式与科学界分享。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准。