Collaborative Research: Deciphering the molecular mechanisms of hormone-like function of terpenoids

合作研究：破译萜类激素类激素功能的分子机制

• 批准号: 2139804
• 负责人: Natalia Doudareva
• 金额: $ 78.53万
• 依托单位: Purdue University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-01 至 2025-07-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2139804&HistoricalAwards=false
• 关键词: Collaborative; Research; Deciphering; molecular; mechanisms; Collaborative; Research; Deciphering; molecular; mechanisms

Plants emit an amazing diversity of volatile organic compounds (VOCs) that play numerous roles in plant growth and development. They are also targets of released compounds as a part of plant-plant communication, as well as plant-insect and plant-microbe interactions. Plants are constantly exposed to atmospheric VOCs and can differentiate and respond to specific cues, therefore VOC perception is an essential part of information exchange. Due to the plethora of biological processes dependent on VOCs, significant progress has been made towards understanding the biosynthesis of plant VOCs and their regulation, and, in recent years, the molecular mechanisms involved in VOC emission. However, to date little is known about how VOCs are perceived by plants and trigger cellular response(s). This project will characterize signaling pathway(s) involved in perception of volatiles and mode of action of volatile signals, particularly terpenoids, in plant growth and development. This research will also answer the long-standing question about the nature of unknown endogenous plant signaling molecule(s). The results will fill important gaps in our understanding of plant olfaction and elucidate the molecular mechanisms underlying volatile perception and responses in plants. The obtained information will be groundbreaking for our understanding of plant interactions in the ecosystem and uncover the differences between volatile perception in plants and that in animals and insects. Given the VOC multifunctionality and overall lack of understanding of volatile perception in plants, this research will identify new targets for metabolic engineering for simultaneous improvement of plant defense and fitness. The proposed research will provide multidisciplinary training to undergraduate and graduate students, and postdoctoral researchers. The educational program will also introduce STEM-based research to a local middle school with minority students. The goals are to improve student enthusiasm, interest, and perceptions about scientific careers.Perception is an essential part of plant-plant, plant-insect and plant-microbe interactions, but the molecular mechanisms remain unknown. The absence of reliable molecular markers of the perception state makes the investigation of plant communication difficult. The recent discovery in petunia flowers of inter-organ aerial transport of volatiles via natural fumigation and hormone-like function of terpenoids, provides an excellent platform for investigating the mode of VOC perception and dissecting signaling cascade(s) involved. Preliminary results in Petunia hybrida flowers led to hypothesis that a karrikin-like signaling pathway is involved in perception of volatile signals via a karrikin-insensitive receptor, PhKAI2ia, and mediates terpene-dependent stigma development. This research will employ an integrative strategy comprised of genetics, molecular biology, biochemistry, metabolic profiling, structural and cell biology to (1) identify the targets of the new KAI2ia-dependent volatile sesquiterpene signaling pathway; (2) perform structure-functional analysis of PhKAI2ia receptor and elucidate the molecular features that determine its substrate specificity/selectivity; and (3) determine whether the KAI2ia-dependent sesquiterpene signalling pathway operates via MAX2 ubiquitin ligase essential for karrikin signaling. This work will uncover yet unknown role(s) of KAI2is in plant VOC perception, illuminate KAI2ia-dependent signaling pathway from organismal to molecular levels and answer the question of how MAX2 ubiquitin ligases deliberate ligand-specific responses.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

植物散发出惊人的挥发性有机化合物（VOC）的惊人多样性，这些化合物在植物生长和发育中起着许多作用。它们也是释放化合物的靶标，作为植物植物通信的一部分，以及植物体和植物 - 微生物相互作用。植物不断暴露于大气中的VOC，可以区分并响应特定的提示，因此VOC感知是信息交换的重要组成部分。由于大量的生物过程取决于VOC，在理解植物VOC及其调节的生物合成方面取得了重大进展，近年来，涉及VOC发射的分子机制。但是，迄今为止，关于植物如何看待VOC和触发细胞反应的知识知之甚少。该项目将表征涉及挥发物感知和挥发性信号（尤其是萜类化合物）在植物生长和发育中的作用方式的信号通路。这项研究还将回答有关未知内源性植物信号分子的性质的长期问题。结果将填补我们对植物嗅觉的理解，并阐明植物中挥发性感知和反应的分子机制。获得的信息将是我们对生态系统中植物相互作用的理解，并揭示植物中挥发性感知与动物和昆虫的差异的开创性。鉴于VOC的多功能性以及对植物中挥发性感知的总体缺乏理解，这项研究将确定代谢工程的新目标，以同时改善植物防御和健身。拟议的研究将为本科生和研究生以及博士后研究人员提供多学科培训。该教育计划还将向与少数学生的当地中学一起引入基于STEM的研究。目标是提高学生对科学职业的热情，兴趣和看法。感知是植物植物，植物 - 植物和植物菌相互作用的重要组成部分，但分子机制仍然未知。缺乏感知状态的可靠分子标记使对植物通信的研究变得困难。最近在矮牵牛花中发现了挥发物通过天然熏蒸的挥发物和萜类似激素的功能的发现，它为研究VOC感知的模式和解剖涉及的信号级联（S）提供了一个绝佳的平台。矮牵牛花的初步结果导致假设，假设Karrikin样信号通路通过Karrikin不敏感的受体Phkai2ia参与了挥发性信号的感知，并介导了萜烯依赖性的污名发展。这项研究将采用一个综合策略，该策略由遗传学，分子生物学，生物化学，代谢分析，结构和细胞生物学组成（1）确定新的KAI2IA依赖性挥发性静态倍烯曲曲板信号传导途径的靶标； （2）对PHKAI2IA受体进行结构功能分析，并阐明确定其底物特异性/选择性的分子特征； （3）确定KAI2IA依赖性倍半萜信号通路是否通过Max2泛素连接酶进行Karrikin信号所必需的。这项工作将揭示Kai2IS在植物VOC感知中的作用但未知的作用，阐明了KAI2IA依赖的信号传导途径，从有机体到分子水平，并回答了Max2泛素连接蛋白旨在故意如何故意配体特定的反应的问题。这些奖项反映了NSF的法定任务和审查的依据，这是通过评估良好的依据。