ME: Collaborative Research: Metabolic Engineering of Floral Scent

ME：合作研究：花香代谢工程

• 批准号: 0331333
• 负责人: Natalia Doudareva
• 金额: $ 27.97万
• 依托单位: Purdue University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-10-01 至 2007-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0331333&HistoricalAwards=false
• 关键词: ME; Collaborative; Research; Metabolic; Engineering

Floral scent is an important character for both crop plants and ornamentals. Floral scent in insect-pollinated, agronomically important plants is crucial for seed and fruit set. For ornamental plants, scent increases the plant's aesthetic value, thus directly benefiting consumers. Recent advances in the isolation of scent biosynthetic genes have opened up the possibility for the genetic manipulation of floral scent. The long-term goal of this research is to use tools of metabolic engineering in combination with metabolic flux analysis and modeling to improve and enhance floral scent in ornamental and cut flowers. Two different approaches will be used to achieve this goal: increasing the amount of pre-existing scent compounds by shifting the metabolic flux, and introducing new scent compounds into the floral scent bouquet. The research is expected to provide a quantitative description of the metabolic networks involved in scent production in petunia and carnation, and uncover the effects of targeted metabolic engineering on steady-state levels of metabolites and fluxes in the pathways. The integration of targeted metabolic engineering with metabolic flux analysis will provide a comprehensive understanding of the regulation of floral scent production in plants. Moreover, this research will fill important gaps in our understanding of floral metabolism in general, and provide powerful modeling tools for general use in the engineering of plant metabolic pathways. The research will also build a foundation for the future metabolic engineering of floral scent of crop plants cultivated for their seed, forage, or food value, with the purpose of increasing their attractiveness to pollinators and thus ensuring efficient pollination. In addition, the project will provide a significant multidisciplinary training of undergraduate and graduate students, as well as postdoctoral associates, including women and minorities, in plant physiology, in vivo isotopic labeling, analytical biochemistry, molecular biology, genetics and integrative modeling. The information and methodologies obtained in this project on secondary metabolism, metabolic engineering, and integrative modeling will be incorporated via WebCT into existing courses at Purdue University and the University of Michigan.

花香是作物植物和装饰物的重要特征。昆虫授粉，农艺上重要的植物中的花香对于种子和水果套装至关重要。对于观赏植物而言，气味提高了植物的美学价值，从而直接使消费者受益。隔离气体生物合成基因的最新进展为花香的遗传操纵开辟了可能性。这项研究的长期目标是将代谢工程的工具与代谢通量分析和建模相结合，以改善和增强观赏和切花的花香。将使用两种不同的方法来实现这一目标：通过转移代谢通量并将新的香气化合物引入花香花束，从而增加了先前存在的气味化合物的量。这项研究有望提供对矮牵牛和康乃馨涉及的代谢网络的定量描述，并发现目标代谢工程对途径中代谢物和通量稳态水平的影响。有针对性的代谢工程与代谢通量分析的整合将对植物中花香生产的调节提供全面的了解。此外，这项研究将填补我们对花卉代谢的理解，并为植物代谢途径的工程提供一般使用的强大建模工具。这项研究还将为未来的代谢工程制定基础，该工程是为了增加其种子，草料或食品价值而种植的农作物植物的花香，目的是提高其对传粉媒介的吸引力，从而确保有效的授粉。此外，该项目还将为本科生和研究生以及包括妇女和少数民族在内的植物生理学，体内同位素标记，分析生物化学，分子生物学，遗传学和综合建模在内提供重大的多学科培训。该项目在该项目中获得的有关次要代谢，代谢工程和综合建模的信息和方法将通过WebCT纳入普渡大学和密歇根大学的现有课程。