Collaborative Research: EAGER--Condensed Matrix Assisted Laser Desorption Ionization (cMALDI) Towards Online Detection of Viral Nucleic Acids and Proteins in Aerosols

合作研究：EAGER——凝聚态基质辅助激光解吸电离 (cMALDI) 在线检测气溶胶中的病毒核酸和蛋白质

• 批准号: 2035870
• 负责人: Kimberly Prather
• 金额: $ 12.5万
• 依托单位: University of California-San Diego Scripps Inst of Oceanography
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-07-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2035870&HistoricalAwards=false
• 关键词: Collaborative; Research; EAGER; Condensed; Matrix

This high-risk, high-reward EAGER project is focused on developing an instrument that can be used to detect viruses and viral fragments associated with atmospheric aerosols in real time in the field. The development of this instrument represents the first step towards answering a number of questions about the modes of atmospheric transport for viruses associated with submicron particles. It is hoped that the instrument will eventually find application in airborne virus monitoring. The instrument to be developed is a condensation matrix assisted laser desorption ionization-aerosol time-of-flight mass spectrometer (cMALDI-ATOFMS). This instrument is expected to provide measurements of the size-resolved number fraction of airborne aerosols containing viruses or viral fragments, using in-flight laser desorption/ionization-time-of-flight mass spectrometry. The effort includes the development and optimization of a three-stage condensation system (cMALDI) to grow submicron, virus-laden particles to the aerodynamically and optically large sizes, needed for ATOFMS optical detection. Laboratory experiments using the cMALDI system will be used to develop calibrations utilizing mixtures of proteins and nucleic acids incorporated into monodisperse submicrometer-to-supermicrometer test particles. These mixtures will be nebulized and used as tests to tune the ATOFMS settings towards biomolecular detection. Individual, bare viruses will be aerosolized via electrospray, such that the signal from a virus is clearly distinguished from background molecules. If successful, these will be the first measurements enabling virus detection at the single aerosol particle level via mass spectrometry.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

这个高风险的高级渴望项目的重点是开发一种可用于实时在现场实时检测与大气气溶胶相关的病毒和病毒碎片。该仪器的开发是回答有关与亚微米颗粒相关的病毒大气传输方式的许多问题的第一步。希望该仪器最终能在空中病毒监测中找到应用。要开发的仪器是冷凝矩阵辅助激光解吸电离 - 飞机上的飞行时间质谱仪（CMALDI-ATOFMS）。预计该仪器将使用飞行中激光解吸/电离时间质谱法提供对含有病毒或病毒片段的空气中气溶胶的尺寸分辨数量的测量。努力包括三阶段冷凝系统（CMALDI）的开发和优化，以将含有病毒的颗粒生长到空气动力学和光学上的大小，这是ATOFMS光学检测所需的。使用CMALDI系统的实验室实验将用于开发校准，利用蛋白质和核酸的混合物中掺入单分散量表至相关测试测试粒子中。 这些混合物将被雾化并用作测试，以调整atofms设置朝着生物分子检测。单个裸病毒将通过电喷雾雾化，从而使病毒的信号显然与背景分子区分开。如果成功的话，这些将是通过质谱法在单个气溶胶颗粒水平上检测病毒检测的首个测量值。该奖项反映了NSF的法定任务，并且使用基金会的智力优点和更广泛的影响审查标准，认为值得通过评估来获得支持。

项目成果

Nanoparticle growth in thermally diffusive sublimation-condensation systems with low vapor pressure solids

低蒸气压固体热扩散升华冷凝系统中纳米颗粒的生长

• DOI: 10.1016/j.jaerosci.2023.106225
• 发表时间: 2023
• 期刊: Journal of Aerosol Science
• 影响因子: 4.5
• 作者: Li, Li;Kimble, Ke’La A.;Mitts, Brock A.;Heilig, Michelle;Qiao, Yuechen;Lee, Jihyeon;Prather, Kimberly A.;Hogan, Christopher J.
• 通讯作者: Hogan, Christopher J.