MRI: Development of a Chemical Ionization Time-of-Flight Mass Spectrometer for On-line Organic Aerosol Analysis

MRI：开发用于在线有机气溶胶分析的化学电离飞行时间质谱仪

• 批准号: 0923581
• 负责人: Kimberly Prather
• 金额: $ 58.65万
• 依托单位: University of California-San Diego
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-08-01 至 2013-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0923581&HistoricalAwards=false
• 关键词: MRI; Development; Chemical; Ionization; Time

This award is funded under the American Recovery and Reinvestment Act of 2009 (Public Law 111-5).This project will develop a novel chemical ionization aerosol time-of-flight mass spectrometer (CI-ATOFMS) that will couple the soft and selective ionization of CI, the ion trapping and transfer properties of an electrodynamic ion funnel (IF), and a high resolution orthogonal acceleration time-of-flight (oa-TOF) mass spectrometer to produce a highly sensitive and selective instrument for characterizing the organic content of atmospheric aerosols. The new instrument will complement existing measurement methods while providing unique insights into the sources and processes leading to the large variety of organic species that exist in aerosols. The analytical capabilities of CI-ATOFMS include: 1) obtaining real-time information on organic species in individual particles; 2) providing information on surface versus bulk species; and 3) selectively analyzing specific classes of compounds. The ability to identify the organic content of single aerosol particles and further classify the aerosols into broad categories based on their source, age, and/or mixing state will make possible a transformative step towards understanding the impact of aerosols on health and the environment. Beyond atmospheric chemistry, performing sensitive on-line CI mass spectrometry on small complex mixtures will open new doors for the analysis of systems such as single cells, particles in water and air (i.e., viruses, phytoplankton, bacteria), pharmaceutical powders, nanomaterials, and allergens such as mold and pollen. On-line analysis has the added benefit of minimal sample preparation, high throughput and rapid screening of compounds, which are current needs in the pharmaceutical industry, organic and biochemical synthesis, early-cancer screening and disease detection, on-line single cell proteomics, and biological mass spectrometry. Working on this instrument development project will provide unique experiences to graduate students, postdoctoral fellows, and undergraduates as the instrument is designed, built, and tested. From an analytical chemistry perspective, students will be trained in the analysis and deconvolution of complex mixtures using state-of-the-art instrumentation and computational tools. All members of the principal investigator's group are proactive in outreach programs to educate the public and they participate regularly in university and community outreach activities. During the summers, the group hosts undergraduates who participate in research programs at the University of California, San Diego that specifically serve underrepresented minorities (African Americans, Chicanos, Latinos, Native Americans, and Pacific Islanders) majoring in chemistry, physics, or other sciences. In addition, the newly developed instrument will receive extensive exposure through public outreach efforts run through the Birch Aquarium at Scripps Institution of Oceanography, which hosts approximately 400,000 visitors per year.

该奖项是根据2009年的《美国回收与再投资法》（公法111-5）资助的。该项目将开发一种新型的化学电离气溶胶飞行器质谱仪（CI-ATOFMS），该项目将使软化和选择性电离将CI，电动力离子漏斗（IF）的离子捕获和转移特性以及高分辨率的正交加速度飞行时间（OA-TOF）质谱仪，以产生高度敏感和选择性的仪器，以表征表征的有机物含量大气气溶胶。新仪器将补充现有的测量方法，同时为导致气溶胶中存在的各种有机物种的来源和过程提供独特的见解。 CI ATOFMS的分析能力包括：1）获取有关单个颗粒中有机物种的实时信息； 2）提供有关表面与散装物种的信息； 3）选择性分析化合物的特定类别。鉴定单个气溶胶颗粒的有机含量并根据其来源，年龄和/或混合状态将气溶胶进一步分类为广泛类别的能力将成为理解气溶胶对健康和环境的影响的变革一步。除了大气化学外，在小型复杂混合物上执行敏感的在线CI质谱法还将为分析诸如单细胞，水和空气中的颗粒（即病毒，植物浮游生物，细菌），药物粉，药物粉，纳米材料，纳米材料，纳米材料，含量，为诸如单细胞，水和空气中的颗粒（即病毒，植物浮游生物）打开新的门。和过敏原，例如霉菌和花粉。在线分析具有最小样品制备，高吞吐量和对化合物快速筛查的额外好处，这些化合物是制药行业的当前需求，有机和生化合成，早期癌症筛查和疾病检测，在线单细胞蛋白质组学，在线单细胞蛋白质组学，和生物质谱法。从事该仪器开发项目的工作将为研究生，博士后研究员和本科生提供独特的体验，并在设计，建造和测试中。从分析化学的角度来看，将使用最先进的仪器和计算工具对学生进行对复杂混合物的分析和解卷的培训。首席调查员小组的所有成员均积极主动在外展计划中教育公众，并定期参加大学和社区外展活动。在夏季，该小组在加利福尼亚大学圣地亚哥大学参加研究计划的本科生，专门为代表性不足的少数群体（非裔美国人，奇卡诺斯，拉丁美洲人，美洲原住民和太平洋岛民）提供服务，专业的化学，物理学或其他科学科学领域为。此外，新开发的工具将通过Scripps海洋学研究所的桦木水族馆进行的公共外展活动将获得广泛的曝光，该机构每年接待约40万名游客。