MRI: Acquisition of a High-Resolution Quadrupole Time-of-Flight Tandem Mass Spectrometer for Advancing Research and Education at the University of Maryland College Park

MRI：马里兰大学学院公园分校购买高分辨率四极杆飞行时间串联质谱仪以推进研究和教育

• 批准号: 2018860
• 负责人: Peter Nemes
• 金额: $ 35万
• 依托单位: University of Maryland, College Park
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-01 至 2023-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2018860&HistoricalAwards=false
• 关键词: MRI; Acquisition; Resolution; Quadrupole; Time

This award is supported by the Major Research Instrumentation and the Chemistry Research Instrumentation programs. Professor Peter Nemes from the University of Maryland College Park and colleagues Daniel Falvey, Lyle Isaacs, Lisa Taneyhill and Scott Juntti are acquiring a high-resolution, high-pressure liquid chromatograph mass spectrometer with electrospray ionization capabilities (HR-HPLC-ESI-MS). In general, mass spectrometry (MS) is one of the key analytical methods used to identify and characterize small quantities of chemical species embedded in complex samples. In a typical experiment, the components flow into a mass spectrometer where they are ionized into ions and the ions' masses are measured. This highly sensitive technique allows the structure of molecules in complex mixtures to be studied. An instrument with a liquid chromatograph can separate mixtures of compounds before they reach the mass spectrometer. In the electrospray technique a high voltage is applied to a liquid to create an aerosol. This voltage is useful to produce ions from large molecules by avoiding the propensity of macromolecules to fragment when ionized. The acquisition strengthens the research infrastructure at the University and regional area. The instrument broadens participation by involving diverse groups of students in research and research training using this modern analytical technique. The acquisition also provides training opportunities to many undergraduate and graduate students as well as postdoctoral fellows at this institution. The new capability to measure both small biological and organic molecules in a shared Mass Spectrometry Facility has a broad impact on scientists and students in the District of Columbia-Maryland-Virginia region through workshops as well as curriculum modernization and collaborations with Bowie State University. The award of the mass spectrometer is aimed at enhancing research and education at all levels. It especially impacts studies of metabolic effectors of embryonic development and hormone metabolite activity in nervous system functions. The instrumentation is also used for research on mitochondrial metabolism in the liver as well as molecular mechanisms in neural crest and dental placode cells. In addition, the MS provides information useful for the search of the next generation enzymatic kinetic isotope effects and for studying binders for the valosine-containing protein (VCP) that segregate protein molecules from large cellular structures. The mass spectrometer is also used to inform the design of efficient syntheses for molecular guest compounds. It is also utilized in investigations of electrophilic nitrogen containing species and their reactivity with proteins and nucleic acids.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该奖项得到了主要的研究工具和化学研究工具计划的支持。马里兰州大学公园和同事丹尼尔·法维（Daniel Falvey），莱尔·艾萨克斯（Lyle Isaacs），丽莎·塔尼（Lisa Taneyhill）和斯科特·扬特（Scott Juntti）的彼得·尼姆斯（Peter Nemes）教授正在获得具有电压电离能力（HR-HPLC-ESI-MS）的高分辨率，高压液相色谱质谱仪的高分辨率。 通常，质谱法（MS）是用于识别和表征嵌入复杂样品中的少量化学物种的关键分析方法之一。在典型的实验中，组件流入质谱仪中，将它们离子化为离子并测量离子的质量。这种高度敏感的技术允许研究复杂混合物中分子的结构。具有液相色谱仪的仪器可以在到达质谱仪之前将其分离化合物的混合物。在电喷雾技术中，将高压施加到液体上以形成气溶胶。该电压可通过避免在电离时避免大分子的倾向来产生来自大分子的离子。此次收购加强了大学和地区地区的研究基础设施。该仪器通过使用这种现代分析技术参与研究和研究培训的各种学生来扩大参与。此次收购还为该机构的许多本科生和研究生以及博士后研究员提供了培训机会。通过研讨会以及与鲍伊州立大学的课程现代化和合作，在共享质谱设施中测量小生物学和有机分子的新能力对哥伦比亚 - 马里兰州 - 维吉尼亚地区的科学家和学生都有广泛的影响。质谱仪的奖励旨在增强各级研究和教育。它特别影响了神经系统功能中胚胎发育和激素代谢产物活性的代谢效应因子的研究。该仪器还用于研究肝脏中的线粒体代谢以及神经rest和牙科底座细胞中的分子机制。此外，MS还提供了用于搜索下一代酶学同位素效应的信息，并用于研究含勇气的蛋白质（VCP）的粘合剂，该蛋白（VCP）将蛋白质分子与大细胞结构分离。质谱仪还用于为分子客体化合物的有效合成设计提供信息。它也用于研究含有物种的亲电氮及其与蛋白质和核酸的反应性。该奖项反映了NSF的法定任务，并被认为是值得通过基金会的知识分子和更广泛影响的评估来审查标准的评估值得支持的。

项目成果

Natural variation and drought-induced differences in metabolite profiles of red oak-leaf and Romaine lettuce play a role in modulating the interaction with Salmonella enterica

红橡叶和长叶生菜的自然变化和干旱引起的代谢物差异在调节与肠沙门氏菌的相互作用中发挥着作用

• DOI: 10.1016/j.ijfoodmicro.2022.109998
• 发表时间: 2023
• 期刊: International Journal of Food Microbiology
• 影响因子: 5.4
• 作者: Liu, Xingchen;Li, Yue;Micallef, Shirley A.
• 通讯作者: Micallef, Shirley A.

New Synthetic Route to Water‐Soluble Prism[5]arene Hosts and Their Molecular Recognition Properties**

水的新合成路线——可溶棱柱[5]芳烃主体及其分子识别特性**

• DOI: 10.1002/chem.202201743
• 发表时间: 2022
• 期刊: Chemistry – A European Journal
• 作者: Zhai, Canjia;Isaacs, Lyle
• 通讯作者: Isaacs, Lyle