Acquisition of a Bruker 11.7T/16cm Preclinical Scanner for Novel MRI/MRSI Studies

采购布鲁克 11.7T/16cm 临床前扫描仪用于新型 MRI/MRSI 研究

• 批准号: 10630511
• 负责人: Dewan Syed Fahmeed Hyder
• 金额: $ 200万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-09-15 至 2024-09-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10630511
• 关键词: 20 year old; 3-Dimensional; Advisory Committees; Anatomy; Animals; Brain; Brain imaging; Cell Nucleus; Communities; Cortical Column; Development; Disease; Education; Educational process of instructing; Extramural Activities; Faculty; Failure; Functional Imaging; Funding; Goals; Helium; Human; Institution; Magnetic Resonance; Magnetic Resonance Imaging; Mentors; Metabolic; Minor; Neurosciences Research; Nuclear; Organoids; Pathogenesis; Performance; Postdoctoral Fellow; Pump; Rat Transgene; Rattus; Research; Research Personnel; Research Project Grants; Resolution; Rodent; Spectrum Analysis; Study models; System; Technical Expertise; Technology; Transgenic Mice; Transgenic Model; Transgenic Organisms; Translations; United States National Institutes of Health; Universities; cost; cryogenics; experience; graduate student; improved; magnetic resonance spectroscopic imaging; metabolic imaging; method development; multimodality; novel; operation; peer; pre-clinical; spectroscopic imaging; structural imaging; tissue fixing; translation to humans; translational model; translational neuroscience; ultra high resolution

Transgenic rodents are the most important translational model for studying pathogenesis and treatment of disease. However magnetic resonance imaging (MRI) and spectroscopy (MRS) as well as spectroscopic imaging (MRSI) studies of transgenic mouse and recently transgenic rat have been limited due to not having sufficient spatial resolution to distinguish key anatomic structures such as cortical columns and layers. With development of ultrahigh field (UHF) animal MR systems, using cryogenic technology for sensitivity enhancement, there is unique opportunity to break this sensitivity barrier allowing ultrahigh resolution functional, structural, and metabolic 3D brain imaging in transgenic models, with a direct path to translation to human studies. The primary goal of this proposal is to install and develop capabilities of a state-of-the-art UHF Bruker 11.7T/16cm preclinical scanner with cryoprobes for 1H and X nuclei at Yale's Magnetic Resonance Research Center (MRRC) for ultrahigh resolution functional, structural, and metabolic imaging in small animals, fixed tissues, and human organoids. Our primary Justification of Need is that this new system will replace an unsupported 20-year old 11.7T pumped magnet with extremely high helium costs that is close to failure. The new 11.7T system will allow our outstanding userbase, and investigators throughout the NIH community, to continue their funded research on a reliable state-of-the-art system, and obtain several-fold sensitivity gains for multi-modal MRI/MRSI via cryogenic coil technology and to answer cutting edge basic and translational neuroscience research questions. The system will be managed by a team of MRI/MRSI researchers with outstanding Technical Expertise and decades of track record in operating and developing UHF systems for MRI/MRSI studies. We have identified 14 Major Users with 18 NIH Research Projects that will most benefit from the new system along with 8 Minor Users. Several are from peer institutions who are performing their preclinical MRI/MRSI projects at Yale MRRC due to our unique method developments. The Administration plan builds on our 35-year experience in facility management, with highly experienced operations, steering, and advisory committees with leading intramural and extramural researchers. Yale University will provide a substantial Institutional Commitment totaling $2,100,000 in matching funds towards the system's cost and pay for complete installation. The system will also benefit Trainee Education based on participation of many of the core MR faculty in mentoring of graduate students and post-doctoral fellows, as well as classroom teaching. By combining UHF with superior gradient performance and cryoprobes for 1H and X nuclei we anticipate an approximately 2.5- to 4-fold gain in sensitivity over our existing unsupported 11.7T system. These improvements will allow translation of our multinuclear and multimodal MRI/MRSI to studies of the transgenic mouse brain and ultrahigh resolution rat brain studies. Thus, the requested 11.7T will fulfill urgent needs for state-of-the-art MRI/MRSI research for NIH-funded investigators at Yale, and elsewhere.

转基因啮齿动物是研究和治疗的最重要的转化模型 疾病。但是磁共振成像（MRI）和光谱法（MRS）以及光谱 由于没有 足够的空间分辨率来区分关键的解剖结构，例如皮质柱和层。和 超高场（UHF）动物MR系统的开发，使用低温技术来敏感 增强，有独特的机会打破这种敏感性障碍，可以超高分辨率 转基因模型中的功能，结构和代谢3D脑成像，直接翻译为 人类研究。该提案的主要目标是安装和发展最先进的功能 UHF Bruker 11.7t/16cm临床前扫描仪，耶鲁的磁共振上有1H和X核的临床前扫描仪 研究中心（MRRC）用于小分辨率的功能，结构和代谢成像 动物，固定组织和人类器官。我们对需求的主要理由是，这个新系统将 用极高的氦气成本替换一个未支撑的20岁的11.7吨泵送磁铁 失败。新的11.7T系统将允许我们出色的用户群和NIH的调查人员 社区，继续他们对可靠的最新系统的资助研究，并获得多个 通过低温线圈技术对多模式MRI/MRSI的敏感性提高，并回答尖端基本和 翻译神经科学研究问题。该系统将由MRI/MRSI团队管理 具有出色技术专长的研究人员以及数十年的运营和发展记录 MRI/MRSI研究的UHF系统。我们已经确定了14位主要用户，其中有18个NIH研究项目 将与8个次要用户一起从新系统中受益最大。几个来自同行机构 由于我们的独特方法，在耶鲁大学MRRC执行临床前MRI/MRSI项目。这 管理计划以我们在设施管理方面的35年经验为基础，经验丰富 领先的壁内和外部研究人员的运营，转向和咨询委员会。耶鲁 大学将提供一项实质性的机构承诺，总计2,100,000美元的资金 该系统的成本和付费用于完整安装。该系统还将受益于基于学员的教育 许多核心先生教师参与指导研究生和博士后研究员， 以及课堂教学。通过将UHF与较高的梯度性能和1H的冷冻探针相结合，然后 x核，我们预计我们现有的未支撑的11.7T的灵敏度大约有2.5至4倍 系统。这些改进将使我们的多核和多模式MRI/MRSI转换为研究 转基因小鼠脑和超高分辨率大鼠脑研究。因此，请求的11.7T将实现 迫切需要在耶鲁大学和其他地方对NIH资助的调查人员进行最先进的MRI/MRSI研究。