Effects of Climate Change Variables on Microbial Autotroph-Heterotroph Carbon Flux

气候变化变量对微生物自养-异养碳通量的影响

基本信息

批准号：
1948104
负责人：
Mary Ann Moran
金额：
$ 98.89万
依托单位：
University of Georgia Research Foundation Inc
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2020
资助国家：
美国
起止时间：
2020-04-01 至 2025-03-31
项目状态：
未结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1948104&HistoricalAwards=false
关键词：
Effects Climate Change Variables Microbial

项目摘要

Phytoplankton in the surface ocean are responsible for roughly half of all photosynthesis on the planet. Much of the organic material created by these photosynthetic organisms is ultimately consumed by diverse marine bacteria with differing preferences for specific types of chemical compounds. This project investigates how climate change (temperature and CO2) might alter the types and amounts of organic compounds produced by different species of marine phytoplankton and the types and amounts of compounds transferred from phytoplankton to marine bacteria. Shifts in organic compounds transferred to bacteria could alter the distribution of bacterial species in the ocean, their growth rates and efficiencies, and flows of energy through the global ocean. This project helps scientists better understand the effects of climate change on marine ecosystems. Two graduate students and a postdoctoral researcher are supported by the project, receiving interdisciplinary training in biology, chemistry, and ocean sciences. Summer research internships in the PIs’ laboratories are offered to AP Biology students enrolled at Cedar Shoals High School in Athens, GA, a school that serves a diverse social and economic community.Much of the bacterial secondary production in the surface ocean is supported by rapid uptake of labile metabolites released from phytoplankton, either directly through excretion and diffusion or indirectly through lysis and predation. This project investigates the effects of two climate change variables (temperature and CO2) on the metabolite pools produced and released by three model phytoplankton species (a diatom, a coccolithophore, and a cyanobacterium) and assesses changes in the composition and fate of metabolites transferred to bacteria. Phytoplankton species are being grown axenically at two different temperatures and CO2 concentrations in a factorial design and endo- and exometabolite composition is determined using NMR. A suite of phytoplankton physiological characteristics is measured and evaluated in the context of metabolite composition. Experiments with heterotrophic bacteria (either model bacteria or natural bacterial communities) are being conducted to assess the effects of climate change variables on metabolite transfer from phytoplankton to marine bacteria. In the first experiment type, bacteria are co-cultured with the phytoplankton at different temperatures and CO2 concentrations, and changes in bacterial gene expression and metabolite concentrations are used to assess shifts in the composition of metabolites transferred. In the second type, bacteria are grown on phytoplankton metabolite pools produced at different temperatures and CO2 concentrations in high-throughput bioassays, and changes in bacterial traits (growth rate, carrying capacity, growth efficiency) resulting from the different climate scenarios are used to indicate changes in metabolite quality. Knowledge of how the heterotrophic processing of phytoplankton metabolites might shift in response to climate change allows better prediction of Earth's future carbon cycle.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

地面海洋中的浮游植物造成了地球上所有光合作用的一半。这些光合生物产生的许多有机材料最终被潜水员海洋细菌消耗，对特定类型的化合物具有不同的偏好。该项目调查了气候变化（温度和二氧化碳）如何改变由不同种类的海洋浮游植物产生的有机化合物的类型和量以及从浮游植物到海洋细菌传递的化合物的类型和量。转移到细菌的有机化合物的变化可能会改变海洋中细菌物种的分布，其生长速度和效率以及通过全球海洋的能量流动。该项目可帮助科学家更好地了解气候变化对海洋生态系统的影响。该项目得到了两名研究生和一名博士后研究人员的支持，并接受了生物学，化学和海洋科学的跨学科培训。夏季研究实习在PIS的实验室提供给AP生物学学生，该学生在佐治亚州雅典的雪松鞋学院入学，这是一所服务于潜水员社会和经济社区的学校。在地面海洋中，细菌中学生产的含量由从Phytoplankton off Indretife and Extreter和Indiction and Indiction and Indiction and Indiction and Indiction and Indiction and Indiction and Indiction and Indiction and Indiction and Indiction and Indiction。该项目研究了两个气候变化变量（温度和二氧化碳）对由三种模型浮游植物物种（一种硅藻，球状石和氰基杆菌）产生和释放的代谢产物池的影响，并评估转移到细菌的分泌物的组成和命运的变化。浮游植物在两个不同的温度下在轴承上生长，并在阶乘设计中二氧化碳浓度，并使用NMR确定内膜代谢物和外量代谢物组成。在代谢产物组成的背景下测量和评估了一组浮游植物生理特征。正在进行异养细菌（模型细菌或天然细菌群落）的实验，以评估气候变化变量对从浮游植物到海洋细菌的代谢物转移的影响。在第一种实验类型中，细菌在不同温度和二氧化碳浓度下与浮游植物共培养，细菌基因表达和代谢产物浓度的变化用于评估转移代谢物的组成的变化。在第二种类型中，细菌是在不同温度下产生的浮游植物代谢物池，在高通量生物测定中生长的二氧化碳浓度，以及细菌性状（生长速率，承载能力，生长效率）的变化，用于通过不同的气候场景引起的，以指示代谢物质量的变化。了解浮游植物代谢产物的异养处理可能会随着气候变化的响应而变化，从而可以更好地预测地球未来的碳循环。该奖项反映了NSF的法定任务，并通过使用基金会的知识分子优点和更广泛的影响审查标准来评估被认为是宝贵的支持。

项目成果

期刊论文数量（7）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

13C NMR metabolomics: J-resolved STOCSY meets INADEQUATE

DOI：
10.1016/j.jmr.2022.107365
发表时间：
2023-01-10
期刊：
JOURNAL OF MAGNETIC RESONANCE
影响因子：
2.2
作者：
Uchimiya，Mario;Olofsson，Malin;Edison，Arthur S.
通讯作者：
Edison，Arthur S.

Assessing the Contribution of Seasonality, Tides, and Microbial Processing to Dissolved Organic Matter Composition Variability in a Southeastern U.S. Estuary

评估季节性、潮汐和微生物处理对美国东南部河口溶解有机物成分变化的影响

DOI：
10.3389/fmars.2021.781580
发表时间：
2021
期刊：
Frontiers in Marine Science
影响因子：
3.7
作者：
Martineac, Rachel P.;Vorobev, Alexey V.;Moran, Mary Ann;Medeiros, Patricia M.
通讯作者：
Medeiros, Patricia M.

Dissolved organic metabolite extraction from high‐salt media

从高盐介质中提取溶解的有机代谢物

DOI：
10.1002/nbm.4797
发表时间：
2022
期刊：
NMR in Biomedicine
影响因子：
2.9
作者：
Holderman, Nicole R.;Ferrer‐González, Frank X.;Glushka, John;Moran, Mary Ann;Edison, Arthur S.
通讯作者：
Edison, Arthur S.

The Ocean's labile DOC supply chain