Bacterial Regulation of Organic Sulfur Cycling in the Ocean: A Genomic Approach

海洋中有机硫循环的细菌调控：基因组方法

基本信息

批准号：
0315200
负责人：
Mary Ann Moran
金额：
$ 48.11万
依托单位：
University of Georgia Research Foundation Inc
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
2004
资助国家：
美国
起止时间：
2004-07-15 至 2009-06-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=0315200&HistoricalAwards=false
关键词：
Bacterial Regulation Organic Sulfur Cycling

项目摘要

A grant has been awarded to Drs. Mary Ann Moran, William B. Whitman, and Anne O. Summers of the University of Georgia, and Ronald P. Kiene of the University of South Alabama to investigate the microbes mediating a key step in the marine sulfur cycle. Dimethylsulfoniopropionate (DMSP) is an abundant organic sulfur compound synthesized by marine algae. When marine bacteria degrade DMSP, they can produce dimethylsulfide (DMS), a volatile compound that is the largest natural source of organic sulfur to the atmosphere. However there are competing bacterial degradation pathways for DMSP, the end products of which play only a minor role in ocean-atmosphere flux of organic sulfur. The genetic basis for these competing degradation pathways and how they are regulated within marine bacterial cells is unknown. Further, it is not understood how environmental conditions in the ocean influence the pathways by which marine bacteria degrade DMSP. In this project, analysis of the genomic sequence of a DMSP-degrading marine bacterium (Silicibacter pomeroyi) will be used in conjunction with molecular biology approaches to identify metabolic and regulatory genes involved in DMSP degradation. Identified genes will be used to retrieve genes with the same function from uncultured and potentially novel marine bacteria from surface waters of coastal and open ocean environments. An important goal of the project is to develop molecular tools for studying the marine bacteria that degrade DMSP in seawater, including how they interact with one another and the environment.

已向博士授予了赠款。佐治亚大学的玛丽·安·莫兰（Mary Ann Moran），威廉·B·惠特曼（William B. Whitman）和安妮·O·萨默斯（Anne O. 二甲基磺胺丙酸酯（DMSP）是由海洋藻类合成的丰富有机硫化合物。当海洋细菌降解DMSP时，它们可以产生二甲基硫化物（DMS），这是一种挥发性化合物，是大气中最大的有机硫的天然自然来源。但是，DMSP的细菌降解途径相互竞争，最终产物在有机硫的海洋 - 大气通量中起较小的作用。这些竞争性降解途径的遗传基础及其在海洋细菌细胞中的调节方式尚不清楚。此外，尚不了解海洋环境条件如何影响海洋细菌降解DMSP的途径。在该项目中，将与分子生物学方法结合使用DMSP降解海洋细菌（硅杆菌Pomeroyi）的基因组序列的分析，以鉴定与DMSP降解有关的代谢和调节基因。鉴定的基因将用于从沿海和开放海洋环境的地表水中从未培养的新型海洋细菌中从具有相同功能的基因中检索基因。该项目的一个重要目标是开发用于研究海水中DMSP降低DMSP的海洋细菌的分子工具，包括它们如何相互作用和环境。