Metabolic Currencies of the Ocean Carbon Cycle

海洋碳循环的代谢货币

基本信息

批准号：
1656311
负责人：
Mary Ann Moran
金额：
$ 74.92万
依托单位：
University of Georgia Research Foundation Inc
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2017
资助国家：
美国
起止时间：
2017-07-01 至 2022-06-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1656311&HistoricalAwards=false
关键词：
Metabolic Currencies Ocean Carbon Cycle

项目摘要

The roles of microbes in cycling carbon and nutrients in the ocean ? the largest biological system on Earth ? were initially described about 40 years ago. Now, it is known that half of Earth's primary production is carried out by marine phytoplankton, and half of that is recycled within weeks by marine bacteria. This proposal is a collaboration between microbiologists and chemists to identify the specific compounds that pass between phytoplankton and bacteria in surface ocean waters. Identifying the key chemicals of the ocean's microbial food web will provide insights into how the marine carbon cycle is regulated, generate data to improve ocean carbon models, and train new scientists at the interface of microbiology and chemistry. Hands-on learning opportunities in microbial ecology will be provided for high school students, both in the classroom and in marine ecosystems of the Georgia coast. Phytoplankton metabolites that sustain the flow of carbon between microbial autotrophs and heterotrophs in the surface ocean carbon cycle will be identified in this project. A matrix of model systems consisting of bacteria-phytoplankton co-cultures will be used as biological assays for key metabolites based on expression patterns of bacterial transporter genes. The chemical identity of candidate metabolites and evaluation of their potential ecological role will be carried out by exometabolomic analysis of co-cultures with bacterial transporter mutants. Both advanced mass spectrometry and NMR will be used for metabolomics analysis, taking advantage of the sensitivity and compound identification strengths of each. The distribution of candidate metabolites in the ocean microbiome and other microbial systems will be characterized by mining environmental sequence datasets for orthologous transporter genes. This project represents a novel approach to identifying metabolites important in microbiome function, compounds often difficult to address with standard chemical approaches because of their low concentrations and high biological demand.

微生物在循环碳和养分中的作用在海洋中？地球上最大的生物系统？最初是大约40年前描述的。现在，众所周知，海洋浮游植物的一半生产是由海洋浮游植物进行的，其中一半是在数周之内通过海洋细菌回收的。该提案是微生物学家和化学家之间的合作，旨在识别浮游植物水域中浮游植物和细菌之间通过的特定化合物。确定海洋微生物食品网的关键化学物质将提供有关如何调节海洋碳循环，生成数据以改善海洋碳模型的信息，并在微生物学和化学的界面上培训新科学家。在课堂和佐治亚海岸的海洋生态系统中，将为微生物生态学的动手学习机会提供。在该项目中，将确定表面海洋碳循环中微生物自身噬菌体和异嗜性植物之间维持碳流动的浮游植物代谢产物。基于细菌转运蛋白基因的表达模式，将使用由细菌 - 浮游植物共培养的模型系统矩阵作为关键代谢产物的生物测定。候选代谢物的化学身份及其潜在生态作用的评估将通过对细菌转运蛋白突变体共培养的外多分析来进行。晚期质谱和NMR都将用于代谢组学分析，利用每个敏感性和复合鉴定强度。候选代谢物在海洋微生物组和其他微生物系统中的分布将以直系同源转运蛋白基因的开采环境序列数据集为特征。该项目代表了一种新的方法来识别在微生物组功能中重要的代谢产物，由于其低浓度和较高的生物学需求，因此通常难以解决标准化学方法的化合物。

项目成果

期刊论文数量（9）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Microdiversity and temporal dynamics of marine bacterial dimethylsulfoniopropionate genes

DOI：
10.1111/1462-2920.14560
发表时间：
2019-05-01
期刊：
ENVIRONMENTAL MICROBIOLOGY
影响因子：
5.1
作者：
Nowinski, Brent;Motard-Cote, Jessie;Moran, Mary Ann
通讯作者：
Moran, Mary Ann

Niche dimensions of a marine bacterium are identified using invasion studies in coastal seawater

DOI：
10.1038/s41564-020-00851-2
发表时间：
2021-01
期刊：
Nature Microbiology
影响因子：
28.3
作者：
Brent Nowinski;M. Moran
通讯作者：
Brent Nowinski;M. Moran

Quantification of Amine- and Alcohol-Containing Metabolites in Saline Samples Using Pre-extraction Benzoyl Chloride Derivatization and Ultrahigh Performance Liquid Chromatography Tandem Mass Spectrometry (UHPLC MS/MS)

使用预提取苯甲酰氯衍生化和超高效液相色谱串联质谱 (UHPLC MS/MS) 定量盐水样品中的胺和醇代谢物

DOI：
10.1021/acs.analchem.0c03769
发表时间：
2021
期刊：
Analytical Chemistry
影响因子：
7.4
作者：
Widner, Brittany;Kido Soule, Melissa C.;Ferrer-González, Frank Xavier;Moran, Mary Ann;Kujawinski, Elizabeth B.
通讯作者：
Kujawinski, Elizabeth B.

Sulfur metabolites in the pelagic ocean