MO: Functional Genomic Investigations of Dissolved Organic Carbon Cyling at the Sapelo Island Microbial Observatory

MO：萨佩洛岛微生物观测站溶解有机碳循环的功能基因组研究

基本信息

批准号：
0702125
负责人：
Mary Ann Moran
金额：
$ 125.01万
依托单位：
University of Georgia Research Foundation Inc
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
2007
资助国家：
美国
起止时间：
2007-09-15 至 2014-08-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=0702125&HistoricalAwards=false
关键词：
MO Functional Genomic Investigations Dissolved

项目摘要

Degradation by marine bacteria of the organic carbon dissolved in coastal seawater is a major process in the ocean carbon cycle. However, the details of this important process seem hopelessly complex. The thousands of distinct compounds that make up this dissolved organic carbon (DOC) pool and the thousands of bacterial species that participate in its metabolism are largely unknown to science, and this greatly impedes efforts to understand the fate of carbon in coastal oceans. In turn, this hinders development of predictive models of carbon cycling in a changing ocean. Overcoming this impediment requires an environmental functional genomics approach. This project will use gene expression patterns in bacterial communities at the Sapelo Island, GA LTER site to identify the components of terrestrial- and marine-derived organic matter that are assimilated and metabolized in the coastal ocean. By sequencing expressed genes directly from seawater at times when ecosystem inputs are the most distinct, the patterns of DOC supply and flux will be discerned. Expression studies of model bacterial strains cultured from coastal communities will be used to identify the biochemical pathways for metabolism of individual carbon compounds and determine the compounds that are preferentially transported and metabolized from the marine DOC pool. The Sapelo Island Microbial Observatory project will improve understanding of the role of land-margin ecosystems in the global carbon cycle, addressing how cellular-level activities mediated by bacteria affect major biogeochemical processes. It will provide opportunities for interdisciplinary training in microbial biogeochemistry and ecological genomics to students and postdoctoral associates, and will sponsor a research experience in microbial biology for promising high school biology students, broadening the participation of underrepresented groups in science. This project will develop novel informatics approaches for microbial ecologists that couple molecular sequence data with environmental context information, and will openly share databases and bioinformatics tools with the scientific community.

溶解在海水中的有机碳的海洋细菌降解是海洋碳循环的主要过程。但是，这个重要过程的细节似乎无望复杂。构成这种溶解的有机碳（DOC）池的数千种不同的化合物和参与其新陈代谢的数千种细菌物种在很大程度上是科学所未知的，这极大地阻碍了理解沿海海洋中碳命运的努力。反过来，这阻碍了不断变化的海洋中碳循环的预测模型的发展。克服这种障碍需要一种环境功能基因组学方法。该项目将在Ga lter遗址的Sapelo岛的细菌群落中使用基因表达模式，以识别在沿海海洋中被吸收和代谢的陆生和海洋衍生有机物的组成部分。通过在生态系统输入最不同的时候直接从海水中表达基因的测序，将辨别DOC供应和通量的模式。对沿海社区培养的细菌菌株的表达研究将用于鉴定单个碳化合物代谢的生化途径，并确定优先从海洋DOC库转运和代谢的化合物。 Sapelo Island微生物天文台项目将提高人们对陆地碎裂生态系统在全球碳循环中的作用的了解，从而解决细菌介导的细胞级活动如何影响主要的生物地球化学过程。它将为学生和博士后伴侣提供微生物生物地球化学和生态基因组学跨学科培训的机会，并将为有希望的高中生物学学生提供微生物生物学研究经验，从而扩大了代表性不足群体在科学中的参与。该项目将为微生物生态学家开发新颖的信息学方法，这些方法将分子序列数据与环境环境信息融为一体，并将与科学界公开共享数据库和生物信息学工具。