MRI: Development of a hybrid mass spectrometry platform with mass-selective optical spectroscopy of cryogenic ions

MRI：开发具有低温离子质量选择光谱的混合质谱平台

• 批准号: 1828190
• 负责人: Mark Johnson
• 金额: $ 50万
• 依托单位: Yale University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-08-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1828190&HistoricalAwards=false
• 关键词: MRI; Development; hybrid; mass; spectrometry

This award is supported by the Major Research Instrumentation and the Chemistry Research Instrumentation programs. Professor Mark Johnson from Yale University and colleagues James Mayer, Scott Miller, Patrick Holland and Nilay Hazari built a new type of mixed mass spectrometer. The spectrometer enables mass-selective isolation of ions for optical spectroscopic characterization after the ions are cooled in a chamber having an inert atmosphere without oxygen or water. The aim of the proposal is to use a hybrid approach in which high resolution mass spectrometry is integrated with optical spectroscopies, such as infrared. The key to this innovation derives from the addition of a cryogenic ion processing stage between the usual ion manipulations (for example, tandem mass spectrometry) and the optical spectrometers that allow the collection of spectra. The instrument includes incorporation of an enclosed, inert environment for ion injection that include sample manipulation in a glovebox and low temperature sample preparation to prolong the ion stability. It is designed as an inert sample orbitrap mass spectrometer with ion spectroscopy (ISOMSIS). In general, mass spectrometry (MS) is one of the key analytical methods used to identify and characterize small quantities of chemical species embedded in complex matrices. In a typical experiment, the components flow into a mass spectrometer where they are ionized into the parent ion and its fragment ions and their masses are measured. This highly sensitive technique allows detection and determination of the structure of molecules in a complex mixture. The addition of a chamber where the ions can be cooled after separation followed by insertion into a spectrometer that measure how light (such as infrared light) interacts with species of interest substantially increases the structural specificity in characterization of unknown compounds and intermediates. The building a new instrumentation is essential to improve economic development and to prepare the new generation of instrumentalists. Students participate and the building process and use the instrument in outreach activities such as that Yale Pathways to Science. The investigators also demonstrate the capabilities afforded by this instrumental platform in activities ranging from outreach to the local community and connections to local chemical industry.The award to build this mixed mass spectrometer is aimed at enhancing research and education at all levels. It especially impacts studies directed to study research is directed to the enzyme nitrogenase model compounds in processes that incorporate nitrogen in plants. The instrument is also used for investigations of the oxygen reduction reaction. In addition, it aids in the discovery of small peptides that catalyze enantioselective reactions, and in the preparation of catalysts with earth-abundant metals to make more environmentally friendly materials that will improve economic development.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该奖项得到了主要的研究工具和化学研究工具计划的支持。耶鲁大学和同事詹姆斯·梅耶（James Mayer），斯科特·米勒（Scott Miller），帕特里克·霍兰德（Patrick Holland）和尼利亚·哈扎里（Nilay Hazari）的马克·约翰逊（Mark Johnson）教授建立了一种新型的混合质谱仪。光谱仪可以在离子在没有氧气或水的惰性气氛中冷却离子后将离子进行质量选择性分离，以进行光谱表征。该提案的目的是使用一种混合方法，其中高分辨率质谱法与光谱（例如红外线）集成在一起。这项创新的关键来自于在通常的离子操作（例如，串联质谱法）和允许频谱收集的光谱仪之间添加低温离子处理阶段。该仪器包括掺入带有离子注射的惰性环境，其中包括在手套箱中的样品操纵和低温样品制备以延长离子稳定性。它被设计为具有离子光谱法（Isomasis）的惰性样品Orbitrap质谱仪。通常，质谱法（MS）是用于识别和表征嵌入复杂基质中的少量化学物种的关键分析方法之一。在典型的实验中，分量流入质谱仪中，在该实验中，测量了它们将其电离为母离子及其片段离子及其质量。这种高度敏感的技术允许检测和确定复杂混合物中分子的结构。分离后可以冷却离子，然后插入光谱仪的腔室的添加，以测量光（例如红外光）与感兴趣的物种相互作用，从而大大提高了未知化合物和中间体表征的结构特异性。该建筑新的工具对于改善经济发展和准备新一代乐器主义者至关重要。学生参加和建筑过程，并将工具用于外展活动，例如耶鲁大学的科学途径。调查人员还证明了该工具平台在从外展到当地社区以及与当地化学工业的联系等活动中提供的功能。建立这种混合质谱仪的奖项旨在增强各级研究和教育。它尤其影响针对研究研究的研究针对在植物中纳入氮的过程中的酶氮酶模型化合物。该仪器还用于研究还原反应。此外，它有助于发现催化对映选择性反应的小肽，并在制备具有地球丰富金属的催化剂中，以制造更加环保的材料，从而改善经济发展。该奖项反映了NSF的法定任务，并通过使用该基金会的知识优点和广泛影响来评估，这是NSF的法定任务，并被认为是值得通过评估的。