Development and study of new generation of ultrabright fluorescent FRET-based sensing nanoparticles

新一代超亮荧光FRET传感纳米粒子的开发与研究

• 批准号: 1605405
• 负责人: Igor Sokolov
• 金额: $ 47.65万
• 依托单位: Tufts University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-08-01 至 2020-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1605405&HistoricalAwards=false
• 关键词: Development; study; new; generation; ultrabright

1605405 Fluorescence is a powerful optical tool in biology and medicine. While the majority of fluorophores are used for imaging, some of them can also be utilized to detect the presence of particular ions. Fluorophores can also be functionalized to adhere to specific chemical structures. While being highly desirable, it is extremely difficult to create molecular fluorophores which can be functionalized and work as sensors. Fluorescent nanoparticles (NPs) offer a unique combination by having a fluorescent sensing core and a functionalizable surface. The PI proposes to develop a new generation of bright fluorescent silica NPs that will work as sensors and be functionalizable with a variety of molecules. The proposed hybrid fluorescent nanoparticles (NPs) are novel and pioneered by the PI. The significantly improved brightness of the NPs could enable many applications that were previously impossible. The proposed super bright hybrid NPs would become a powerful tool for many biomedical applications that are not addressable with fluorescent dyes. The educational impact will include the direct exposure of the research to his graduate student and his post-doc on the grant, the PI has and will continue to involve undergraduates in the research. He will incorporate the research findings into existing courses he teaches and will maintain existing outreach efforts and public presentations in high schools.

1605405 荧光是生物学和医学中强大的光学工具。虽然大多数荧光团用于成像，但其中一些荧光团也可用于检测特定离子的存在。荧光团还可以被功能化以粘附到特定的化学结构。虽然非常理想，但创建可功能化并用作传感器的分子荧光团却极其困难。荧光纳米颗粒 (NP) 具有荧光传感核心和功能化表面，提供了独特的组合。该 PI 提议开发新一代明亮的荧光二氧化硅纳米粒子，该纳米粒子将用作传感器并可与多种分子功能化。所提出的混合荧光纳米粒子 (NP) 是新颖的，由 PI 首创。纳米粒子亮度的显着提高可以实现许多以前不可能的应用。所提出的超亮混合纳米粒子将成为许多荧光染料无法解决的生物医学应用的强大工具。教育影响将包括直接向他的研究生和博士后展示研究成果，PI 已经并将继续让本科生参与研究。他将把研究结果纳入他所教授的现有课程中，并将维持现有的外展工作和在高中的公开演讲。