Collaborative Research: IDBR Type A: QSTORM-AO-Wavefront-shaping light-sheet microscopy with photoswitchable quantum dots for superresolution imaging in thick tissue

合作研究：IDBR A 型：带有光控量子点的 QSTORM-AO-波前整形光片显微镜，用于厚组织中的超分辨率成像

• 批准号: 1555470
• 负责人: Jessica Winter
• 金额: $ 19.64万
• 依托单位: Ohio State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-05-01 至 2019-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1555470&HistoricalAwards=false
• 关键词: Collaborative; Research; IDBR; Type; QSTORM

An award is made to the University of Georgia to develop a novel super-resolution microscope for biological research. For students and early-career researchers, this close collaboration at the intersection of biology, physics, optics, and chemical engineering will provide an invaluable training experience, and it will be enriched with professional development in interdisciplinary science communication skills. All team members will contribute to the development of an online team blog, lab notebook, and public website that will capture the progress of the research within a broader historical, scientific, and visual context, curated by the Boston Museum of Science. The Museum of Science team will also disseminate this work to thousands of science museum visitors, students, and the broader community with live presentations, demonstrations, podcasts, and a short NOVA-style film on the quest to see more clearly and deeply into the mysteries of life. The film will be directed by a multiple award-winning NOVA producer.This instrument will combine holographic imaging using high resolution spatial light modulators and new techniques for correcting distortion induced by light scattering in thick biological tissue to achieve molecular-scale resolution inside organisms important for modern biological research, such as fruit flies and zebrafish. At The Ohio State University, semiconductor nanotechnology will be combined with DNA origami to create intensely bright and switchable light emitters, individually targetable to selected molecules operating within biological systems. This novel combination of high-precision technologies will provide investigators an unprecedented view of important subcellular biological processes, such as neuronal signaling in intact organisms, potentially contributing to breakthroughs in areas of molecular and systems biology, biological engineering, medicine, and human health.

向佐治亚大学颁发了奖励，以开发一种新型的生物学研究超分辨率显微镜。对于学生和早期研究人员，这种在生物学，物理，光学和化学工程交集的密切合作将提供宝贵的培训经验，并且将丰富跨学科科学沟通技巧的专业发展。所有团队成员都将为在线团队博客，实验室笔记本和公共网站的开发做出贡献，该网站将在波士顿科学博物馆策划的更广泛的历史，科学和视觉环境中捕捉研究的进步。科学博物馆还将向成千上万的科学博物馆游客，学生和更广泛的社区传播这项工作，并通过现场演示，示范，播客，以及一部简短的Nova风格电影，以更清楚，深入了解生活之谜。这部电影将由多个屡获殊荣的NOVA生产商指导。本仪器将使用高分辨率的空间光调节器和新技术结合全息成像，以纠正厚物生物组织中的光散射引起的变形，以实现分子规模的分辨率，以实现有机体内部对现代生物学研究的重要性。在俄亥俄州立大学，半导体纳米技术将与DNA折纸结合使用，以创建强烈明亮，可切换的光发射器，该发射器可单独地定位于在生物系统中运行的选定分子。 这种新颖的高精度技术组合将为研究人员提供对重要亚细胞生物学过程的前所未有的观点，例如完整生物中的神经元信号传导，有可能有助于分子和系统生物学，生物学工程，医学，医学和人类健康的突破。