Collaborative Research: IDBR: Type A: The Nanosizer: A New Tool for the Photochemical Fabrication of Bioactive Nanoarrays

合作研究：IDBR：A 型：Nanosizer：生物活性纳米阵列光化学制造的新工具

• 批准号: 1353823
• 负责人: Adam Braunschweig
• 金额: $ 33万
• 依托单位: University of Miami
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-08-01 至 2016-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1353823&HistoricalAwards=false
• 关键词: Collaborative; Research; IDBR; Type; Nanosizer

This award is being made jointly by two Programs- (1) Instrument Development for Biological Research, in the Division of Biological Infrastructure (Biological Sciences Directorate), and (2) Nano-Biosensing, in the Division of Chemical, Bioengineering, Environmental and Transport Systems (Engineering Directorate). Non Technical Description:Researchers at Northwestern University, and the University of Miami will develop an instrument to produce nanoarrays of biologically active probes. This new tool will produce combinatorial arrays of oligonucleotides and oligopeptides with sub-micrometer feature diameters over large areas (10's of square centimeters). The work will achieve order of magnitude improvements in feature size, production rate, and cost over current technologies providing access to fundamental biological experiments that could not otherwise be undertaken, including new ways to measure gene or protein expression at the single cell level. The development is a highly interdisciplinary effort which combines chemistry, materials science, engineering, and nanotechnology. Project activities will also include summer internships for undergraduates from minority-serving four year colleges, and a range of other outreach activities involving the Museum of Science and Industry in Chicago to promote scientific awareness, and with Breakthrough Miami to create internship opportunities for high school students from financially disadvantaged communities.Technical Description:An instrument to create ultradense patterns of biologically active molecules will be developed to model the spatial and chemical complexity of biological systems or create arrays for determining gene or protein expression at the single-cell level. The goal of the proposed activity is to combine new surface chemistries with new instrument capabilities to make a go-to tool for the in situ synthesis of combinatorial arrays of oligonucleotides or oligopeptides with feature size and shape control over square centimeter areas The Nanosizer is enabled by two breakthrough advances to recently emerge from the Mirkin and Braunschweig groups, namely 1) the development of massively parallel pen arrays that are individually addressable by light, thereby combining the advantages of massively parallel pen arrays with photolithography (near- and farfield), and 2) new surface immobilization chemistries and photochemistries for the rapid printing of molecules onto surfaces. This project combines these two features into an automated platform that can photoactivate a surface with individually addressable tips, expose it to a range of reagents, and repeat for several cycles to create spatially encoded combinatorial arrays or nanopatterns of biologically active molecules on surfaces. When the appropriate instrumentation milestones are reached, initial filings of patent applications will be made through institutional commercialization offices, and partnerships will be sought with instrumentation manufacturers. Early dissemination of the research findings will occur through conferences followed by full reports in scientific journals. When milestones of significant public interest are reached, institutional public relations offices will be contacted to produce a press release. CAD drawings for photomasks for preparing tip arrays and microfluidic cells will be made available on the websites of the PIs so researchers can implement the Nanosizer on existing AFMs.

该奖项由两个计划（1）生物学研究工具开发，生物基础设施（生物科学局）共同颁发，以及（2）纳米生物传感器在化学，生物工程，环境和运输系统（工程局）的纳米生物传感器。非技术描述：西北大学的研究人员和迈阿密大学将开发一种生产生物活性探针纳米阵列的工具。该新工具将产生寡核苷酸和寡肽的组合阵列，并在大面积（正方形10厘米）上具有亚微米特征计直径。这项工作将在特征大小，生产率和成本方面的数量级改善，而当前技术可以访问无法进行基本生物学实验，包括在单细胞水平上测量基因或蛋白质表达的新方法。该发展是一项高度跨学科的工作，结合了化学，材料科学，工程和纳米技术。项目活动还将包括为少数派服务的四年制大学提供的本科生的暑期实习，以及涉及芝加哥科学和工业博物馆的其他一系列外展活动，以促进科学意识，并与迈阿密的突破性实习机会，为经济上偏离的社区创造的高中生，以创造出来的工具，以创造出造型的工具。生物系统的复杂性或创建用于确定单细胞水平基因或蛋白质表达的阵列。提出活动的目的是将新的表面化学与新的仪器能力结合起来，以制造一种用于现场合成寡核苷酸组合阵列或寡肽组合阵列的特征大小和形状控制纳米剂的特征大小和形状控制的纳米疗法，从可以通过光单独寻址，从而将大规模平行的笔阵列与光刻阵列（接近和远场）的优势相结合，以及2）新的表面固定化学和光化学，以快速将分子在表面上快速打印。该项目将这两个功能结合到一个自动化平台中，该平台可以将表面带有单独寻址的尖端，将其暴露于一系列试剂中，然后重复几个循环，以在表面上创建具有空间编码的生物活性分子的空间编码的组合阵列或纳米模板。当达到适当的仪器里程碑时，将通过机构商业化办公室进行专利申请的初步申请，并将与仪器制造商寻求合作伙伴关系。研究结果的早期传播将通过会议进行，然后在科学期刊上进行完整的报告。当达成极大的公共利益的里程碑时，将联系机构公共关系办公室，以制作新闻稿。用于制备尖端阵列和微流体细胞的光罩的CAD图将在PIS的网站上提供，以便研究人员可以在现有的AFM上实施纳米剂。