EAGER: Complete 3D imaging of needle biopsy to diagnose pancreatic cancer

EAGER：完整的针活检 3D 成像诊断胰腺癌

• 批准号: 1212540
• 负责人: Eric Seibel
• 金额: $ 30万
• 依托单位: University of Washington
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-03-15 至 2015-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1212540&HistoricalAwards=false
• 关键词: EAGER; Complete; 3D; imaging; needle

1212540SeibelA new method for processing and imaging of thin-needle core biopsy (TNCB) of resected tissue containing pancreatic cancer (animal and human) is proposed. TNCB specimens of 0.25mm diameter will be obtained by a custom-designed coring device, while larger 0.40mm cores will be taken with a new commercial biopsy needle. Corresponding fine needle aspirate (FNA) specimens will be taken at or alongside the TNCB sample sites. While maintaining TNCB tissue within a tube or microfluidic chamber, the specimen will be fixed, stained, and optically cleared. Absorption dyes of hematoxylin and eosin (H&E) will provide transmission image contrast for the morphological structure of cells and tissue.Fluorescence dye will be imaged from various immunohistological targets, such as proteins expressed at the base epithelial membrane to help determine invasion and protein expression. Optical projection tomographic microscopy (OPTM) will be used to generate 3D multimodal images of biopsy tissue. Although the length of the tissue core will be longer than the microscope field of view (FOV), different regions of the tissue will be imaged and stitched together by image processing. Both 2D and 3D visualization of the entire TNCB specimen will be provided to a pathologist using a custom computer interface to manipulate the 3D dataset. Sensitivity and specificity of cancer diagnosis will be made using tissue-slice pathology as the gold standard. Comparative evaluation of pancreatic cancer diagnosis will be made between TNCB imaged with the OPTM versus conventional FNA. This new technique is expected not only to detect the presence of neoplastic cells but detect invasion of tissue by cancer cells.

1212540Seibel 提出了一种对含有胰腺癌（动物和人类）的切除组织进行细针核心活检（TNCB）处理和成像的新方法。直径 0.25 毫米的 TNCB 样本将通过定制设计的取芯装置获得，而更大的 0.40 毫米核心将使用新的商用活检针获取。相应的细针抽吸 (FNA) 标本将在 TNCB 采样点或其旁边采集。在将 TNCB 组织保留在管或微流体室内的同时，样本将被固定、染色和光学透明。苏木精和伊红 (H&E) 吸收染料将为细胞和组织的形态结构提供透射图像对比度。荧光染料将对各种免疫组织学目标（例如上皮基底膜表达的蛋白质）进行成像，以帮助确定侵袭和蛋白质表达。光学投影断层显微术 (OPTM) 将用于生成活检组织的 3D 多模态图像。尽管组织核心的长度将比显微镜视野（FOV）长，但组织的不同区域将被成像并通过图像处理缝合在一起。整个 TNCB 标本的 2D 和 3D 可视化将提供给病理学家，使用定制计算机界面来操作 3D 数据集。癌症诊断的敏感性和特异性将使用组织切片病理学作为金标准。将使用 OPTM 成像的 TNCB 与传统 FNA 成像的胰腺癌诊断进行比较评估。这项新技术不仅有望检测肿瘤细胞的存在，还能检测癌细胞对组织的侵袭。