EAGER: Complete 3D imaging of needle biopsy to diagnose pancreatic cancer

EAGER：完整的针活检 3D 成像诊断胰腺癌

• 批准号: 1212540
• 负责人: Eric Seibel
• 金额: $ 30万
• 依托单位: University of Washington
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2012
• 资助国家: 美国
• 起止时间: 2012-03-15 至 2015-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1212540&HistoricalAwards=false
• 关键词: EAGER; Complete; 3D; imaging; needle

1212540SeibelA new method for processing and imaging of thin-needle core biopsy (TNCB) of resected tissue containing pancreatic cancer (animal and human) is proposed. TNCB specimens of 0.25mm diameter will be obtained by a custom-designed coring device, while larger 0.40mm cores will be taken with a new commercial biopsy needle. Corresponding fine needle aspirate (FNA) specimens will be taken at or alongside the TNCB sample sites. While maintaining TNCB tissue within a tube or microfluidic chamber, the specimen will be fixed, stained, and optically cleared. Absorption dyes of hematoxylin and eosin (H&E) will provide transmission image contrast for the morphological structure of cells and tissue.Fluorescence dye will be imaged from various immunohistological targets, such as proteins expressed at the base epithelial membrane to help determine invasion and protein expression. Optical projection tomographic microscopy (OPTM) will be used to generate 3D multimodal images of biopsy tissue. Although the length of the tissue core will be longer than the microscope field of view (FOV), different regions of the tissue will be imaged and stitched together by image processing. Both 2D and 3D visualization of the entire TNCB specimen will be provided to a pathologist using a custom computer interface to manipulate the 3D dataset. Sensitivity and specificity of cancer diagnosis will be made using tissue-slice pathology as the gold standard. Comparative evaluation of pancreatic cancer diagnosis will be made between TNCB imaged with the OPTM versus conventional FNA. This new technique is expected not only to detect the presence of neoplastic cells but detect invasion of tissue by cancer cells.

提出了1212540地下，用于处理含有胰腺癌（动物和人类）的切除组织的薄针核活检（TNCB）的新方法。直径为0.25mm的TNCB标本将通过定制设计的芯设备获得，而较大的0.40mm核心将使用新的商业活检针头进行。相应的细针吸入（FNA）样品将在TNCB样品位点或与TNCB样品位置一起取。在将TNCB组织保持在管或微流体室内的同时，将固定，染色并光学清除样品。苏木精和曙红（H＆E）的吸收染料将为细胞和组织的形态结构提供传播图像对比度。荧光染料将从各种免疫组织学靶标中成像，例如在基础上皮膜上表达的蛋白质，以帮助确定入侵和蛋白质表达。光投射断层扫描显微镜（OPTM）将用于生成活检组织的3D多模式图像。尽管组织核的长度将比显微镜视野（FOV）更长，但将通过图像处理对组织的不同区域进行成像和缝合。整个TNCB标本的2D和3D可视化都将使用自定义的计算机接口向病理学家提供，以操纵3D数据集。癌症诊断的敏感性和特异性将使用组织片病理学作为黄金标准。将使用OPTM与常规FNA成像的TNCB对胰腺癌诊断的比较评估。预计这种新技术不仅可以检测到肿瘤细胞的存在，还可以检测癌细胞对组织的侵袭。