Adaptomics of neofunctionalization: analysis of GORDITA-like genes in Brassicaceae

新功能化的适应组学：十字花科 GORDITA 样基因的分析

• 批准号: 197965843
• 负责人: Professorin Dr. Annette Becker
• 金额: --
• 依托单位: Arbeitsgruppe Spezielle Botanik
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2011
• 资助国家: 德国
• 起止时间: 2010-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/197965843?language=en
• 关键词: Adaptomics; neofunctionalization; analysis; GORDITA; like

The project investigates how the origin of a single developmental control gene, GORDITA (GOA), by gene duplication and sequence divergence contributed to plant fitness and adaptation in the Brassicaceae. While the ancestral Bsister genes (ABS-type genes) are involved in ovule development, the derived GOA-type genes acquired a new expression domain and function in fruit development. By comprehensive comparative analyses involving GOA-type and ABS-type genes from diverse Brassicales species we will investigate when the lineage that led to extant GOA-type genes originated, and by which molecular mechanisms these genes acquired their unique expression patterns, domain structures, and function during fruit development. To better understand how the ancestors of GOA escaped ‘Ohno’s dilemma’ we will analyze the molecular evolution in the different branches of Bsister genes during the phylogeny of Brassicales employing in silico methods. Using protein-protein interaction studies we will investigate how changes in the protein structure changed the protein interaction network of GOA in comparison of ABS. To assess how the origin of GOA-type genes contributed to the adaptation of Brassicaceae species we will determine proxies of plant fitness under greenhouse conditions using transgenic knock-out plants, and will do preparative work for future determination of plant fitness under natural growth conditions using non-transgenic mutants.

该项目研究了单一发育控制基因 GORDITA (GOA) 的起源如何通过基因复制和序列差异促进十字花科植物的适应和适应，而祖先 Bsister 基因（ABS 型基因）则参与胚珠发育。 ，衍生的GOA型基因在果实发育中获得了新的表达域和功能，通过对来自不同Brassicales物种的GOA型和ABS型基因的综合比较分析，我们将研究何时导致现存 GOA 型基因起源的谱系，以及这些基因在果实发育过程中获得其独特表达模式、结构域结构和功能的分子机制。为了更好地了解 GOA 的祖先如何摆脱“Ohno 困境”，我们将分析这些基因。使用计算机方法研究芸苔目系统发育过程中 Bister 基因不同分支的分子进化，我们将研究蛋白质结构的变化如何改变 GOA 的蛋白质相互作用网络。 ABS. 为了评估 GOA 型基因的起源如何促进十字花科物种的适应，我们将使用转基因敲除植物确定温室条件下植物适应性的代理，并为未来自然生长下植物适应性的确定做好准备工作。使用非转基因突变体的条件。