Comparative transcriptome analysis and phenotypic monitoring of Trifolium pratense (Fabaceae) under land use scenarios (TRATSCH II - Trifolium pratense RNA analysis to screen character heterogeneity)

土地利用情景下红车轴草（豆科）的比较转录组分析和表型监测（TRATSCH II - 红车轴草 RNA 分析，筛选性状异质性）

基本信息

批准号：
252282964
负责人：
Professorin Dr. Annette Becker
金额：
--
依托单位：
Arbeitsgruppe Spezielle Botanik
依托单位国家：
德国
项目类别：
Infrastructure Priority Programmes
财政年份：
2014
资助国家：
德国
起止时间：
2013-12-31 至 2019-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/252282964?language=en
关键词：
Comparative transcriptome analysis phenotypic monitoring

项目摘要

Plants adapt to changes in the environment as they can be invoked by agricultural land use with an astonishing morphological flexibility caused by differential gene expression and genetic adaptation. However, our knowledge about organismal responses to land use with regard to underlying molecular genetic processes and changes in genetic diversity is scarce. In the proposed project, we aim to investigate the organisms genetic responses to environmental perturbations (mowing and fertilization on grassland systems). Red clover (Trifolium pratense) is an important agricultural forage species in grasslands, as it is a rich protein source, and has reduced nitrogen fertilization requirements due to N2-fixation. Both attributes contribute to reduce the ecological footprint of grassland based agriculture. T. pratense occurs on all Biodiversity Exploratory grassland sites and in TRATSCH I we showed that T. pratense (i) responds to mowing with specific morphological changes; (ii) we identified genes that are differentially expressed between plots and others, that show a mowing-specific response, and (iii) established a mRNA-seq fingerprinting protocol which allows for a broader sampling scheme and associations with environmental variables. Thus, we are now able to interrelate landscape genetics towards landscape genomics to better understand a species genome functioning in an environmental context. We propose to extend our data collection and analyses to all Exploratories to investigate how site/treatment/environmental specific transcriptional changes of T. pratense are. We further propose to correlate T. pratense differential gene expression with environmental data. Functional analysis of the mowing-response genes will improve our understanding of differentially expressed developmental regulators that coordinate the phenotype and regrowth of biomass upon mowing treatments. We test different management regimes to achieve high protein content with the lowest possible fertilization regime to minimize the environmental footprint in grassland based agriculture. Last, we hypothesize that epigenetic modifications are involved in the regulation of morphological changes in response to mowing, and to test the hypothesis we will observe quantitative and temporal shifts in methylation pattern of the developmental genes genomic loci.

植物适应环境的变化，因为它们可以通过农业土地的使用引起，并由差异基因表达和遗传适应引起的惊人形态柔韧性。然而，我们对潜在的分子遗传过程和遗传多样性变化的生物对土地使用的反应的了解很少。在拟议的项目中，我们旨在研究生物对环境扰动的遗传反应（草原系统上的割草和施肥）。红三叶草（三叶植物）是草原中重要的农业饲料物种，因为它是一种丰富的蛋白质来源，并且由于N2固定而降低了氮肥要求。这两种属性都有助于减少基于草地的农业的生态足迹。 T. Pratense发生在所有生物多样性探索性草地遗址上，在Tratsch I中，我们表明the（i）对割草有特定的形态变化做出反应；（ii）我们确定了在图和其他图之间差异表达的基因，这些基因表现出特定于割草的响应，并且（iii）建立了mRNA-Seq指纹指纹方案，该方案允许更广泛的采样方案并与环境变量的关联。因此，我们现在能够将景观遗传学与景观基因组学相关，以更好地理解在环境环境中起作用的物种基因组。我们建议将数据收集和分析扩展到所有探索者，以研究T. Pratense的站点/治疗/环境特定的转录变化。我们进一步建议将T. pratense差异基因表达与环境数据相关联。割草响应基因的功能分析将提高我们对差异表达的发育调节剂的理解，这些调节因子在割草处理中协调生物质的表型和再生。我们测试不同的管理状态，以实现具有最低施肥制度的高蛋白质含量，以最大程度地减少基于草地的农业的环境足迹。最后，我们假设表观遗传修饰与割草的形态变化有关，并且为了检验假设，我们将观察到发育基因基因组基因座的甲基化模式的定量和时间变化。