Functional diversification of class1 KNOX genes in basal eudicots

基础真双子叶植物 1 类 KNOX 基因的功能多样化

• 批准号: 19309724
• 负责人: Professorin Dr. Annette Becker
• 金额: --
• 依托单位: Arbeitsgruppe Spezielle Botanik
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2005
• 资助国家: 德国
• 起止时间: 2004-12-31 至 2009-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/19309724?language=en
• 关键词: Functional; diversification; class1; KNOX; genes

KNOX genes are a family of homeobox genes that are essential regulators of plant shoot development. KNOX genes are required for continued growth of the shoot apical meristem, and leaf formation requires KNOX genes to be downregulated. In several angiosperm lineages, an additional function of KNOX genes in the control of leaf dissection has evolved. However, evolutionary changes of KNOX gene action associated with modifications of leaf forms are not well understood. This project aims to characterize this small gene family and its role in leaf development in the basal eudicot plant Eschscholzia californica. Eschscholzia provides a unique system because of its dissected leaves, its phylogenetic position between core eudicots and basal angiosperms, and the availability of developmental genetic technologies. In addition, contrasting developmental patterns in related species invite within-family comparisons. To understand dissected leaf development, we will firstly characterize the class 1 KNOX genes and their expression patterns in Eschscholzia and the related genus Chelidonium. Secondly, phenotypic effects of modulated expression will be determined in Eschscholzia, using Agrobacterium-mediated transformation and Virus-induced gene silencing. To further assess differences in the action of KNOX genes between Eschscholzia and other species, and between dissected and simple leaves, the interaction with other genes and with phytohormones will be studied. Comparisons with other species will promote our understanding of how diversification of KNOX genes is implicated in morphological evolution.

诺克斯基因是同源基因家族，是植物芽发育的重要调节剂。 knox基因是持续生长的芽顶分生组织所必需的，叶片形成需要下调诺克斯基因。在几个被子植物谱系中，诺克斯基因在叶片解剖控制中的附加功能已经发展。然而，与叶片形式修饰相关的诺克斯基因作用的进化变化尚不清楚。该项目旨在表征这个小基因家族及其在基底Eudicot工厂Eschscholzia Californica中在叶片发育中的作用。 Eschscholzia提供了一个独特的系统，因为其解剖的叶子，核心eudicot和基础被子植物之间的系统发育位置以及发育遗传技术的可用性。另外，相关物种中的对比的发育模式会引起家庭内比较。为了了解解剖的叶片发育，我们首先将表征1类诺克斯基因及其在Eschscholzia及其相关属Chelidonium属中的表达模式。其次，使用农杆菌介导的转化和病毒诱导的基因沉默，将在Eschscholzia中确定调制表达的表型效应。为了进一步评估Eschscholzia和其他物种之间诺克斯基因作用的差异，将研究与其他基因以及与植物激素的相互作用。与其他物种的比较将促进我们对诺克斯基因多样化如何与形态进化有关的理解。