Functional diversification of class1 KNOX genes in basal eudicots

基础真双子叶植物 1 类 KNOX 基因的功能多样化

• 批准号: 19309724
• 负责人: Professorin Dr. Annette Becker
• 金额: --
• 依托单位: Arbeitsgruppe Spezielle Botanik
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2005
• 资助国家: 德国
• 起止时间: 2004-12-31 至 2009-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/19309724?language=en
• 关键词: Functional; diversification; class1; KNOX; genes

KNOX genes are a family of homeobox genes that are essential regulators of plant shoot development. KNOX genes are required for continued growth of the shoot apical meristem, and leaf formation requires KNOX genes to be downregulated. In several angiosperm lineages, an additional function of KNOX genes in the control of leaf dissection has evolved. However, evolutionary changes of KNOX gene action associated with modifications of leaf forms are not well understood. This project aims to characterize this small gene family and its role in leaf development in the basal eudicot plant Eschscholzia californica. Eschscholzia provides a unique system because of its dissected leaves, its phylogenetic position between core eudicots and basal angiosperms, and the availability of developmental genetic technologies. In addition, contrasting developmental patterns in related species invite within-family comparisons. To understand dissected leaf development, we will firstly characterize the class 1 KNOX genes and their expression patterns in Eschscholzia and the related genus Chelidonium. Secondly, phenotypic effects of modulated expression will be determined in Eschscholzia, using Agrobacterium-mediated transformation and Virus-induced gene silencing. To further assess differences in the action of KNOX genes between Eschscholzia and other species, and between dissected and simple leaves, the interaction with other genes and with phytohormones will be studied. Comparisons with other species will promote our understanding of how diversification of KNOX genes is implicated in morphological evolution.

KNOX 基因是同源框基因家族，是植物芽发育的重要调节因子。 KNOX 基因是茎尖分生组织持续生长所必需的，叶子的形成需要 KNOX 基因下调。在一些被子植物谱系中，KNOX 基因控制叶子解剖的附加功能已经进化。然而，与叶子形状改变相关的 KNOX 基因作用的进化变化尚不清楚。该项目旨在表征这个小基因家族及其在基部真双子叶植物加州苜蓿叶子发育中的作用。 Eschscholzia 因其解剖的叶子、其在核心真双子叶植物和基部被子植物之间的系统发育位置以及发育遗传技术的可用性而提供了一个独特的系统。此外，相关物种的不同发育模式会引发科内比较。为了了解解剖叶片的发育，我们将首先表征 1 类 KNOX 基因及其在 Eschscholzia 和相关白屈菜属中的表达模式。其次，将使用农杆菌介导的转化和病毒诱导的基因沉默来确定埃希氏菌中调节表达的表型效应。为了进一步评估 KNOX 基因在 Eschscholzia 和其他物种之间以及解剖叶和简单叶之间的作用差异，将研究与其他基因和植物激素的相互作用。与其他物种的比较将促进我们对 KNOX 基因的多样化如何参与形态进化的理解。