Engineering Neofunctionalization in a Model Triterpene Synthase (HEMMINGS_U17DTP1)

三萜合酶模型中的工程新功能化 (HEMMINGS_U17DTP1)

基本信息

批准号：
1937543
负责人：
金额：
--
依托单位：
University of East Anglia
依托单位国家：
英国
项目类别：
Studentship
财政年份：
2017
资助国家：
英国
起止时间：
2017 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=studentship-1937543
关键词：
Engineering Neofunctionalization Model Triterpene Synthase

项目摘要

Enzyme engineering plays a major role in the UK bioeconomy as a route to the discovery of novel compounds and the production of high-value chemicals. The global market for industrial enzymes is predicted to reach $7bn by 2018.Plant-derived natural products form a large set of compounds frequently found to have pharmaceutical, agricultural and industrial biotechnology applications yet their commercial exploitation is often limited by their synthetic intractability. In a collaboration between the groups of Dr. Andrew Hemmings (University of East Anglia) and Professor Anne Osbourn (John Innes Centre) a major project is underway to develop engineered natural product biosynthetic enzymes capable of producing novel, exploitable compoundsA PhD research studentship has become available to carry out exciting research aimed at reengineering a critical triterpene biosynthetic enzyme. The successful candidate will play a central role in attempts to engineer the catalytic properties of this target. To achieve this they will receive training in cutting edge methods of protein engineering, molecular and structural biology, analytical biochemistry and computational modelling. The research will make extensive use of local facilities at UEA including the Centre for Structural and Molecular Biochemistry (CMSB) and at the John Innes Centre.

酶工程作为发现新型化合物和生产高价值化学品的途径，在英国生物经济中发挥着重要作用。预计到 2018 年，全球工业酶市场将达到 70 亿美元。植物源性天然产物形成了大量化合物，经常被发现具有制药、农业和工业生物技术应用，但它们的商业开发往往因其合成难处理性而受到限制。在 Andrew Hemmings 博士（东安格利亚大学）和 Anne Osbourn 教授（约翰英尼斯中心）的团队之间的合作中，正在进行一个重大项目，开发能够生产新型、可利用化合物的工程天然产物生物合成酶，博士研究生奖学金已成为可用于开展旨在重新设计关键三萜生物合成酶的令人兴奋的研究。成功的候选者将在设计该目标的催化特性的尝试中发挥核心作用。为了实现这一目标，他们将接受蛋白质工程、分子和结构生物学、分析生物化学和计算建模等前沿方法的培训。该研究将广泛利用东安格利亚大学的当地设施，包括结构和分子生物化学中心 (CMSB) 和约翰·英尼斯中心。